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The MGH NextGen Sequencing Core

The NextGen Sequencing Core at MGH is your resource for sequencing on the Illumina HiSeq platform, constructing libraries, providing data analysis services, and answering any questions you may have, whether or not you are an MGH affiliate. This website is an overview of the services the Core offers, as well as a good resource for general NextGen sequencing questions and protocols.

http://nextgen.mgh.harvard.edu/

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The MGH NextGen Sequencing Core | nextgen.mgh.harvard.edu Reviews
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The NextGen Sequencing Core at MGH is your resource for sequencing on the Illumina HiSeq platform, constructing libraries, providing data analysis services, and answering any questions you may have, whether or not you are an MGH affiliate. This website is an overview of the services the Core offers, as well as a good resource for general NextGen sequencing questions and protocols.
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The MGH NextGen Sequencing Core | nextgen.mgh.harvard.edu Reviews

https://nextgen.mgh.harvard.edu

The NextGen Sequencing Core at MGH is your resource for sequencing on the Illumina HiSeq platform, constructing libraries, providing data analysis services, and answering any questions you may have, whether or not you are an MGH affiliate. This website is an overview of the services the Core offers, as well as a good resource for general NextGen sequencing questions and protocols.

INTERNAL PAGES

nextgen.mgh.harvard.edu nextgen.mgh.harvard.edu
1

The MGH NextGen Sequencing Core | Core Services

http://nextgen.mgh.harvard.edu/OrderingInfo.html

Ordering for MGH laboratories and staff (internal) is done through the Galaxy server, while external customers may submit samples by completing a spreadsheet and sending it to our core via email. For internal and external customers, we have specific guidelines which we ask that you follow in order to insure that your results are the best possible quality (see links below for more information). To work out a solution. View the complete checklist of pre-submission requirements,. Make sure your samples foll...

2

The MGH NextGen Sequencing Core

http://nextgen.mgh.harvard.edu/index.html

3

The MGH NextGen Sequencing Core | Core Services

http://nextgen.mgh.harvard.edu/Resources.html

Click on the links below for downloadable protocols and other resources. For best results and to save the document, do not open in Google Chrome. For submitting samples and retrieving data). TruSeq DNA Sample Prep. TruSeq RNA Sample Prep. TruSeq Small RNA Sample Prep. TruSeq Exome Enrichment Guide. TruSeq is the most-recently released line of library prep kits from Illumina.). Genomic DNA Sample Prep. GEX DpnII Sample Prep. GEX NlaIII Sample Prep. MatePair v2 2-5kn Sample Prep.

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The MGH NextGen Sequencing Core | Core Services

http://nextgen.mgh.harvard.edu/About.html

The NextGen Core was founded as a collaboration between the Molecular Biology, Center for Human Genetics Research, and Center for Computational Biology departments. Currently, the Core operates using a single Ilumina HiSeq instrument, accompanied by Illumina's cBot for cluster generation. This upgrade from our Genome Analyzer II doubled our capacity and greatly increased the data amount, quality, and stability over extra-long reads. Some of these applications are:. 8226; Genomic sequencing or resequencing.

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The MGH NextGen Sequencing Core | Core Services

http://nextgen.mgh.harvard.edu/Pricing.html

Basic pricing for NextGen library construction and sequencing services is given below. Additional fees apply for downstream Bioinformatics analysis services or consultations. Please contact us. For specific pricing information on Bioinformatics consulting. Illumina HiSeq Sequencing in High-Output mode. Illumina HiSeq sequencing in Rapid mode. Single 50 cBot Duo. Paired 50 cBot Duo. Single 100 cBot Duo. Paired 100 cBot Duo. Single 150 cBot Duo. Paired 150 cBot Duo. Paired 25 cBot Duo.

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molbio.mgh.harvard.edu molbio.mgh.harvard.edu

Laboratories | Department of Molecular Biology

http://molbio.mgh.harvard.edu/laboratories

History and Current Status. DMB occupies the sixth and seventh floors of the Simches Research Building on the main MGH Campus with over 40,000 net square feet with integration of its faculty within research groups in the new thematic centers at Simches. Drs. Hung, Szostak and Altshuler occupy space in MGH ‘thematic centers’ that are immediately adjacent to the core DMB space. Relationship to the Department of Genetics.

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Site map | Department of Molecular Biology

http://molbio.mgh.harvard.edu/sitemap

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Blower Laboratory | Department of Molecular Biology

http://molbio.mgh.harvard.edu/laboratories/blower

Michael Blower, Ph.D. Department of Molecular Biology. Boston, MA 02114. The Blower lab is interested in the intersection of microtubules, RNA, and mitosis. The primary focus is on understanding four major areas of RNA localization during mitosis:. What are the cis acting sequences and trans-acting proteins that recognize, transport, and anchor localized RNAs to microtubules during mitosis? How does localization of specific mRNAs to microtubules affect mitosis and development? Mike Blower attended Miami ...

molbio.mgh.harvard.edu molbio.mgh.harvard.edu

Avruch People | Department of Molecular Biology

http://molbio.mgh.harvard.edu/members/avruch

Joseph Avruch, M.D. Department of Molecular Biology. Boston, MA 02114. Past members ▼. Click on a member's name or photo to see their profile and publications. Research Associate in Medicine. Click on a member's name or photo to see their profile and publications. Back to top ▲.

molbio.mgh.harvard.edu molbio.mgh.harvard.edu

Hochedlinger People | Department of Molecular Biology

http://molbio.mgh.harvard.edu/members/hochedlinger

Konrad Hochedlinger, Ph.D. Department of Molecular Biology. Boston, MA 02114. Administrative Assistant: Joanne Desmond. Past members ▼. Click on a member's name or photo to see their profile and publications. Click on a member's name or photo to see their profile and publications. Back to top ▲.

molbio.mgh.harvard.edu molbio.mgh.harvard.edu

Hung People | Department of Molecular Biology

http://molbio.mgh.harvard.edu/members/hung

Deborah Hung, M.D./Ph.D. Department of Molecular Biology. Boston, MA 02114. Past members ▼. Click on a member's name or photo to see their profile and publications. Assistant in Molecular Biology. Associate Director, Tuberculosis Operations. Click on a member's name or photo to see their profile and publications. Back to top ▲.

molbio.mgh.harvard.edu molbio.mgh.harvard.edu

Ausubel Laboratory | Department of Molecular Biology

http://molbio.mgh.harvard.edu/laboratories/ausubel

Frederick Ausubel, Ph.D. Department of Molecular Biology. Boston, MA 02114. The responses by which multicellular eukaryotic hosts defend themselves from pathogen attack have only recently begun to be subjected to rigorous genetic analysis. This sort of analysis requires a tractable genetic system for the host and well-characterized host-pathogen interactions. Whereas vertebrates, with the possible exception of zebrafish, are generally unsuitable for such studies, Arabidopsis thaliana. Systems. The ra...

molbio.mgh.harvard.edu molbio.mgh.harvard.edu

Blower People | Department of Molecular Biology

http://molbio.mgh.harvard.edu/members/blower

Michael Blower, Ph.D. Department of Molecular Biology. Boston, MA 02114. Past members ▼. Click on a member's name or photo to see their profile and publications. Click on a member's name or photo to see their profile and publications. Back to top ▲.

molbio.mgh.harvard.edu molbio.mgh.harvard.edu

Ausubel People | Department of Molecular Biology

http://molbio.mgh.harvard.edu/members/ausubel

Frederick Ausubel, Ph.D. Department of Molecular Biology. Boston, MA 02114. Past members ▼. Click on a member's name or photo to see their profile and publications. Assistant in Molecular Biology. Click on a member's name or photo to see their profile and publications. Frans J. de Bruijn. Assistant in Molecular Biology. Jonathan D. G. Jones. Assistant in Molecular Biology. Assistant in Molecular Biology. Raj Mohan Raja Muthiah. Back to top ▲.

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