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Next Generation Sequencing and Data Analysis

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Next Generation Sequencing and Data Analysis | ngsda.blogspot.com Reviews
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Notes and scratches
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1 notes and scratches
2 partitions evenly
3 change id
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5 port=$ expr ${i} 9092
6 change log file
7 done
8 kafka start sh
9 start zookeeper
10 5 open
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Next Generation Sequencing and Data Analysis: June 2014

http://ngsda.blogspot.com/2014_06_01_archive.html

Next Generation Sequencing and Data Analysis. Monday, June 9, 2014. VirtualBox 4.3.12 configuration. File- "Preferences"- Click "Network" in left panel - Add a new NAT network. CIDR: 192.168.0.0/24. Click the "Port forwarding" add port "22" in Ipv4. Host IP "10.2.2.72" (run "cmd"- "ipconfig" to get it). Guest IP "192.168.0.4" (The IP of the primary virtual machine). For each virtual machine, in "Settings"- "network"- "Adapter1"- "NAT Network" choose "MyNAT". The Daddy of Maomao and Doudou.

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Next Generation Sequencing and Data Analysis: August 2014

http://ngsda.blogspot.com/2014_08_01_archive.html

Next Generation Sequencing and Data Analysis. Wednesday, August 27, 2014. AWS AMI for Hadoop DataNode. Create an AWS AMI as the snapshot for hadoop datanode. This image should contain all necessary tools, packages and libraries. To be used by the pipeline and your hadoop-application. Assume the we already started a Ubuntu14.04-64-PV instance. Public IP for the instance is 12.34.56.78 and our private key file was saved as. AWS IP=12.34.56.78. Step1 log in our AWS instance. Step2 install basic packages.

3

Next Generation Sequencing and Data Analysis: July 2014

http://ngsda.blogspot.com/2014_07_01_archive.html

Next Generation Sequencing and Data Analysis. Monday, July 28, 2014. Split the BAM file by chromosome then process it with GATK's UnifiedGenotyper. To parallel the execution of genotyping, one possible solution is to split the BAM files by chromosome (the safe method). For example, we have one big BAM file named "A.bam". Now we want to use GATK's UnifiedGenotyper to call variants on "A.bam". The command may looks like this:. Now call GATK's UnifiedGenotyper:. Time java -jar /home/hadoop/bio/app/gatk/3&#4...

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Next Generation Sequencing and Data Analysis: March 2015

http://ngsda.blogspot.com/2015_03_01_archive.html

Next Generation Sequencing and Data Analysis. Friday, March 6, 2015. Download from BaseSpace using R. Under shell sudo apt-get update sudo apt-get install libcurl4-gnutls-dev sudo R #under R source('http:/ bioconductor.org/biocLite.R') biocLite('RCurl') biocLite('BaseSpaceR') quit() #Start R again library(BaseSpaceR) ACCESS TOKEN. Reference: http:/ seqanswers.com/forums/showthread.php? The Daddy of Maomao and Doudou. Subscribe to: Posts (Atom). The Daddy of Maomao and Doudou. View my complete profile.

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Next Generation Sequencing and Data Analysis: Download sequencing data from illumina's basespace

http://ngsda.blogspot.com/2015/06/download-sequencing-data-from-illuminas.html

Next Generation Sequencing and Data Analysis. Friday, June 12, 2015. Download sequencing data from illumina's basespace. Spent 30 mins creating a script to download sequencing data from illumina's BaseSpace. By using its REST API. Here is the code:. Https:/ gist.github.com/anonymous/91c5accc3988cb233347. The Daddy of Maomao and Doudou. Subscribe to: Post Comments (Atom). The Daddy of Maomao and Doudou. View my complete profile. Download sequencing data from illuminas basespace.

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