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No DNA Control

Repository for my contemplations of transmission genetics and natural transformation in Haemophilus infuenzae, as well as notes on becoming a hack bioinformaticist

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No DNA Control | nodnacontrol.blogspot.com Reviews
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Repository for my contemplations of transmission genetics and natural transformation in Haemophilus infuenzae, as well as notes on becoming a hack bioinformaticist
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1 no dna control
2 phase ii
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5 meeting was awesome
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7 recombinants
8 as possible
9 we need replication
10 we need controls
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no dna control,phase ii,image credit,the human microbiome,meeting was awesome,here's,recombinants,as possible,we need replication,we need controls,continued,posted by chang,1 comments,aacck,0 comments,link,hi blog,selected set 72,controls 3,great,and here
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No DNA Control | nodnacontrol.blogspot.com Reviews

https://nodnacontrol.blogspot.com

Repository for my contemplations of transmission genetics and natural transformation in Haemophilus infuenzae, as well as notes on becoming a hack bioinformaticist

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1

No DNA Control: Repression of competence induction by purines

http://nodnacontrol.blogspot.com/2010/03/repression-of-competence-induction-by.html

Repository for my contemplations of transmission genetics and natural transformation in Haemophilus infuenzae, as well as notes on becoming a hack bioinformaticist. Sunday, March 14, 2010. Repression of competence induction by purines. First, a schematic depiction of how I interpret what we already know the induction of the competence regulon:. Gene Sxy protein alters the binding specificity of CRP to also bind at CRP-S promoters, thereby inducing competence gene expression. MRNA transcript contains a st...

2

No DNA Control: They're eating our DNA!!!

http://nodnacontrol.blogspot.com/2009/06/eating-our-dna.html

Repository for my contemplations of transmission genetics and natural transformation in Haemophilus infuenzae, as well as notes on becoming a hack bioinformaticist. Tuesday, June 16, 2009. They're eating our DNA! I am in the midst of proposal-writing, which makes blogging tougher, but when I hit a writing-block at the end of the day, I decided to dally with a random thing I'd been meaning to figure out. Maybe it is, but I went ahead and used TAGSCAN. That means Haemophilus influenzae. To produce control ...

3

No DNA Control: Degenerate Oligos

http://nodnacontrol.blogspot.com/2009/05/rosie-and-i-have-been-working-our-way.html

Repository for my contemplations of transmission genetics and natural transformation in Haemophilus infuenzae, as well as notes on becoming a hack bioinformaticist. Tuesday, May 5, 2009. Rosie and I have been working our way through editing my unfunded NIH postdoc proposal. There's a lot of work to do on it, but I think we'll make it excellent by the time we're done. This is our first aim (so far):. The idea is to feed Haemophilus. To start with, I want to try and outline the basic properties of the dege...

4

No DNA Control: grantgrantgrant

http://nodnacontrol.blogspot.com/2010/02/grantgrantgrant.html

Repository for my contemplations of transmission genetics and natural transformation in Haemophilus infuenzae, as well as notes on becoming a hack bioinformaticist. Friday, February 5, 2010. So another grant out-of-the way for now; another one almost done; and my own postdoc re-application in the works… A brief respite… Maybe it’s time to blog. My figures here might have been a little degraded on their journey into the blog). But until our little sequencing experiment, we could only infer the extent of t...

5

No DNA Control: Mining some old array data

http://nodnacontrol.blogspot.com/2010/04/mining-some-old-array-data.html

Repository for my contemplations of transmission genetics and natural transformation in Haemophilus infuenzae, as well as notes on becoming a hack bioinformaticist. Tuesday, April 13, 2010. Mining some old array data. I looked at a time course dataset, in which expression was monitored over the course of growth in sBHI and after transfer to MIV. I also looked at a single one-off array comparing purR- to purR strains growing in late-log cAMP. Conclusion: Nothing we didn’t already suspect, but it&#82...

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Re-re-streaking | infoenzae

https://infoenzae.wordpress.com/2011/06/12/re-re-streaking

Sex in bacteria: Is it a planned conception or an occasional accidental byproduct of sex trade workers gathering resources? Leave a Reply Cancel reply. Enter your comment here. Fill in your details below or click an icon to log in:. Address never made public). You are commenting using your WordPress.com account. ( Log Out. You are commenting using your Twitter account. ( Log Out. You are commenting using your Facebook account. ( Log Out. You are commenting using your Google account. ( Log Out.

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infoenzae | Sex in bacteria: Is it a planned conception or an occasional accidental byproduct of sex trade workers gathering resources? | Page 2

https://infoenzae.wordpress.com/page/2

Sex in bacteria: Is it a planned conception or an occasional accidental byproduct of sex trade workers gathering resources? Yesterday I transformed E.coli with the ligation product of TA cloning. The results look good! All the controls worked as expected. I plated on X-gal(for CFU) and X-gal Ampicilin(For Transformants) LB plates. No IPTG was used. The controls I did are as follows:. Pic 1: Contaminant white colony. From plate 1, a rec1 CFU plate with x-gal but no Ampicilin. Pic 3 A lonely colony that is...

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Digesting clones | infoenzae

https://infoenzae.wordpress.com/2011/06/16/digesting-clones

Sex in bacteria: Is it a planned conception or an occasional accidental byproduct of sex trade workers gathering resources? 10 000 units is the total amount of units you get when you purchase a tube from NEB. 1 unit is needed to digest 1 ug of DNA in 1 hour. I did not need to leave the digest overnight. Haemophilus transformation check results. Is Competence a candidate system to study quantum biology? Leave a Reply Cancel reply. Enter your comment here. Address never made public).

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RSS feed | infoenzae

https://infoenzae.wordpress.com/2011/06/14/rss-feed

Sex in bacteria: Is it a planned conception or an occasional accidental byproduct of sex trade workers gathering resources? I added a RSS feed to the sidebar. The feed displays the titles of the seven most recent papers on google scholar that include the terms “Haemophilus influenzae competence”. Instructions. On how to make your own feed courtesy of http:/ nsaunders.wordpress.com/. Haemophilus transformation check results. Jun 15, 2011. Leave a Reply Cancel reply. Enter your comment here.

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Last Post | infoenzae

https://infoenzae.wordpress.com/2011/07/19/last-post

Sex in bacteria: Is it a planned conception or an occasional accidental byproduct of sex trade workers gathering resources? This blog is defunct and will not likely be updated anymore. For anyone finding their way to this website through doing similar research, please feel free to contact me by email if you have any questions. What will I be doing? The problems with modern agriculture. 8211; Benefits and criticisms, current state of industry. 8211; Benefits, highlights, how GMOs are made, and current pro...

infoenzae.wordpress.com infoenzae.wordpress.com

Todays experiments | infoenzae

https://infoenzae.wordpress.com/2011/06/13/todays-experiments

Sex in bacteria: Is it a planned conception or an occasional accidental byproduct of sex trade workers gathering resources? The re-restreak plates looked good this morning so I’m running 12 test colonies through PCR to see if any of them amplify the 2815bp Rec1 gene region. I poured 2 more LB X-gal Ampicillin plates to use as grid plates to save the colonies I checked by PCR. I’m wrapping up the final haemophilus transformation assay. Leave a Reply Cancel reply. Enter your comment here. Http:/ nodnacontr...

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About | infoenzae

https://infoenzae.wordpress.com/about

Sex in bacteria: Is it a planned conception or an occasional accidental byproduct of sex trade workers gathering resources? What infoenzae means: It’s a spin on the name of our excellent model organism,. Dnogas at gmail.com. Office 2.553 Life Science institute. Leave a Reply Cancel reply. Enter your comment here. Fill in your details below or click an icon to log in:. Address never made public). You are commenting using your WordPress.com account. ( Log Out. Notify me of new comments via email.

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dnogas | infoenzae

https://infoenzae.wordpress.com/author/dnogas

Sex in bacteria: Is it a planned conception or an occasional accidental byproduct of sex trade workers gathering resources? This blog is defunct and will not likely be updated anymore. For anyone finding their way to this website through doing similar research, please feel free to contact me by email if you have any questions. What will I be doing? The problems with modern agriculture. 8211; Benefits and criticisms, current state of industry. 8211; Benefits, highlights, how GMOs are made, and current pro...

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No DNA Control

Repository for my contemplations of transmission genetics and natural transformation in Haemophilus infuenzae, as well as notes on becoming a hack bioinformaticist. Wednesday, March 16, 2011. Here's a 16S phylogeny of bacteria that live in the human airway:. Some other places on the body. Anyways, cool stuff. So my ears are itching, because yesterday the local genome centre was doing quality control on our libraries of Haemophilus influenzae. The basic experiment is really straightforward: (1) Incubate d...

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