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Online Biological Protocol

Friday, October 17, 2008. 3M sodium acetate preparation. Preparation of 3M sodium acetate (50ml):. 3M sodium acetate is used to precipitate DNA or RNA from ethanol. For 50 ml,. 20412 g Sodium acetate [ CH3COONa•3H2O, trihydrate]. 6 ml acetic acid to pH, adjust the pH to 5.2 using Glacial acetic acid. Add dH2O to make 50 ml. Links to this post. Monday, October 6, 2008. Then you can purify samples in two ways,. A) Phenol-chloroform extraction,. B) Using kit column,. 3 Spin in a microfuge for 3 min. 8 Measu...

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Friday, October 17, 2008. 3M sodium acetate preparation. Preparation of 3M sodium acetate (50ml):. 3M sodium acetate is used to precipitate DNA or RNA from ethanol. For 50 ml,. 20412 g Sodium acetate [ CH3COONa•3H2O, trihydrate]. 6 ml acetic acid to pH, adjust the pH to 5.2 using Glacial acetic acid. Add dH2O to make 50 ml. Links to this post. Monday, October 6, 2008. Then you can purify samples in two ways,. A) Phenol-chloroform extraction,. B) Using kit column,. 3 Spin in a microfuge for 3 min. 8 Measu...

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2 jewel

3 36 comments

4 labels sodium acetate

5 dna /rna purification

6 a phenol chloroform extraction

7 materials

8 methods

9 see also

10 1 comment

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posted by,jewel,36 comments,labels sodium acetate,dna /rna purification,a phenol chloroform extraction,materials,methods,see also,1 comment,labels enzyme,eppendorf,merceptoethanol,purification,rnase,sodium acetate,eid mubarak,2 comments,labels eid,mubarak

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Online Biological Protocol | obprotocol.blogspot.com Reviews

https://obprotocol.blogspot.com

Friday, October 17, 2008. 3M sodium acetate preparation. Preparation of 3M sodium acetate (50ml):. 3M sodium acetate is used to precipitate DNA or RNA from ethanol. For 50 ml,. 20412 g Sodium acetate [ CH3COONa•3H2O, trihydrate]. 6 ml acetic acid to pH, adjust the pH to 5.2 using Glacial acetic acid. Add dH2O to make 50 ml. Links to this post. Monday, October 6, 2008. Then you can purify samples in two ways,. A) Phenol-chloroform extraction,. B) Using kit column,. 3 Spin in a microfuge for 3 min. 8 Measu...

INTERNAL PAGES

obprotocol.blogspot.com

1

Online Biological Protocol: August 2008

http://obprotocol.blogspot.com/2008_08_01_archive.html

Friday, August 29, 2008. Send your request and protocol to me. abdullahuu@gmail.com. Links to this post. Subscribe to: Posts (Atom). View my complete profile.

2

Online Biological Protocol: What is DNA? How to extract DNA?

http://obprotocol.blogspot.com/2008/09/what-is-dna-how-to-extract-dna.html

Monday, September 1, 2008. How to extract DNA? DNA has two different ends 5'end and 3' end. DNA is double helix. Two complimentary strand stay together. Adenine (A) always binds with thymine (T) and Guanine (G) always binds with cytosine (C) by hydrogen bonding. In 1962, James D. Watson and Francis Crick received Nobel prize for describing the structure of DNA in Physiology of Medicine. Basic principles to extract DNA from cells are:. 61485; Clean raw cells. 61485; precipitate DNA by using alcohol.

3

Online Biological Protocol: Eid Mubarak.....

http://obprotocol.blogspot.com/2008/10/eid-mubarak.html

Wednesday, October 1, 2008. One thing i am little bit confused about that is, DNA/RNA purification, both can be done in the same ways? October 7, 2008 at 6:55 AM. S really a great and helpful piece of information [url=http:/ pandoracharmsukmall.co.uk]pandora charms sale[/url] . I? M satisfied that you simply shared this helpful info with us. [url=http:/ pandoracharmsukmall.co.uk]cheap pandora charms[/url] , Please keep us up to date like this. Thank you for sharing. January 23, 2013 at 5:50 AM.

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Online Biological Protocol: Loading dye or buffer

http://obprotocol.blogspot.com/2008/09/loading-dye-or-buffer.html

Saturday, September 27, 2008. Loading dye or buffer. In gel electrophoresis experiment loading dye or buffer is widely used with DNA and RNA to follow the migration of samples. Typical loading buffer composed of two dyes bromophenol blue and xylene cyanol FF. It can be used for both agarose and polyacrylamide gel. Composition of 6x loading dye or buffer:. 003% bromophenol blue,. 10 mM Tris-HCl (pH 7.6),. 003% xylene cyanol FF,. 25mg bromophenol blue (0.25%),. 25mg xylene cyanol (0.25%),. 4g sucrose (40%),.

5

Online Biological Protocol: TBE and TE buffer preparation

http://obprotocol.blogspot.com/2008/09/tbe-and-te-buffer-preparation.html

Tuesday, September 9, 2008. TBE and TE buffer preparation. TBE buffer is a solution of Tris base, boric acid and EDTA. This buffer mostly used in DNA electrophoresis experiment. Recipe for 10x TBE (TrisBorateEDTA) buffer:. 05M EDTA : 40ml. Add distilled water and adjust the volume to 1 liter. TE buffer protects DNA or RNA from degradation. 1ml of 1M Tris-HCL (pH 7.5-8). 02ml of 0.5M EDTA (pH 8). Add 988ml of distilled water to make 1 liter 1x TE buffer. My blog - play free casino slots win real money.

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Online Biological Protocol

Friday, October 17, 2008. 3M sodium acetate preparation. Preparation of 3M sodium acetate (50ml):. 3M sodium acetate is used to precipitate DNA or RNA from ethanol. For 50 ml,. 20412 g Sodium acetate [ CH3COONa•3H2O, trihydrate]. 6 ml acetic acid to pH, adjust the pH to 5.2 using Glacial acetic acid. Add dH2O to make 50 ml. Links to this post. Monday, October 6, 2008. Then you can purify samples in two ways,. A) Phenol-chloroform extraction,. B) Using kit column,. 3 Spin in a microfuge for 3 min. 8 Measu...

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