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原代细胞-武汉奥斯丹生物科技有限公司
http://www.outstandingbio.com/product/html?16.html
OriCellTM 神经元系列产品取自18.5 天胎鼠或者新生鼠的海马区和大脑皮层，原代直接冻存。 OriCell 小鼠胚胎成纤维细胞取材于怀孕13.5 天的ICR 小鼠胚胎，使用其专用完全培养基贴壁培养至第一代，并且经过γ-射线照射处理后冻存。
细胞冻存和复苏实验-武汉奥斯丹生物科技有限公司
http://www.outstandingbio.com/news/html?411.html
作者 管理员 发布于 2015-12-13 15:14:08 文字 大. 细胞冻存和复苏可以 1 用于生物学保种 2 用于医学上干细胞研究 3 用于传代培养。 细胞冻存和复苏可以 1 用于生物学保种 2 用于医学上干细胞研究 3 用于传代培养。 D-Hanks液 小牛血清 培养液 青霉素 链霉素 胰蛋白酶 HCl NaHCO3 DMSO 甘油. 微量加样枪 吸头 离心管 冻存管 枪头 胶塞 移液管 玻璃瓶 红血球计数板 记号笔 医用橡皮膏 移液枪 超净工作台离心机 恒温水浴箱 冰箱 倒置相差显微镜 培养箱 液氮冰箱. 1 仪器 净化工作台、 离心机、恒温水浴箱、冰箱 4 、-20 、-70 、倒置相差显微镜、培养箱、液氮冰箱。 2 玻璃器皿 吸管 弯头、直头 、 培养瓶、玻璃瓶 250ml、100ml 、废液缸。 3 塑料器皿 吸头、枪头、胶塞、移液管 10ml 、15ml离心管、冻存管 1 2ml。 5 试剂 D-Hanks液、小牛血清、培养液、双抗 青霉素、链霉素 、胰蛋白酶 0.08% 、1NHCl、7.4%NaHCO3、DMSO 分析纯 或无色新鲜甘油。 3 离心1 000 rpm，5 min。
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通用型无蛋白非程序冻存液 -武汉奥斯丹生物科技有限公司
http://www.outstandingbio.com/news/html?412.html
作者 管理员 发布于 2015-12-01 15:14:57 文字 大. 冻存液特点 即用型产品，方便快捷 复苏率更高达 90%，远超普通冻存液的70%的效率 无需程序冻存，细胞可直接置于- 80 C冰箱完成冻存过程，省事省时 无血清无蛋白配方，成分明确，不影响细胞的生长和分化潜能及其它高级应用. 1 选择处于对数生长期的细胞，按照常用的方法收集细胞于离心管中，按照培养细胞密度和所用细胞冻存管的尺寸计算所需冻存细胞数 参考数量 5 105 至 5 106 cells/mL。 2 取相当于所需细胞数的细胞悬浮液量，置于离心管中，离心收集培养细胞 参考离心条件 4 ，250 g，离心3-5 min。 6 将冻存管直接置于-80 冰箱中，24 h后移入液氮长期保存。 3 离心收集培养细胞 参考离心条件 4 ，250 g，离心3-5 min)，吸去上清液。 7 镜检后放置于37 ，5% CO2，饱和湿度的培养箱中继续培养。 无菌检测 细菌、真菌和支原体检测 PH测试 渗透压检测 内毒素检测. 附件下载 CBPI0204A5 NCPF-10001 7gZS7NP.pdf.
细胞系-武汉奥斯丹生物科技有限公司
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