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the Phiel Lab

Second, we are interested in understanding the mechanism of the ketogenic diet. Which is used to treat epilepsy in children whose seizures are not controlled by drugs. Through an active collaboration with the Division of Neurology and the Ketogenic Clinic. Here at Nationwide Children's Hospital. See the article here. If you would like to contact us, please email Dr. Phiel at Christopher.Phiel@nationwidechildrens.org. Or call at 614 722-5916. Funding provided by NIH/NIA (R01) AG031883.

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the Phiel Lab | phiellab.com Reviews
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Second, we are interested in understanding the mechanism of the ketogenic diet. Which is used to treat epilepsy in children whose seizures are not controlled by drugs. Through an active collaboration with the Division of Neurology and the Ketogenic Clinic. Here at Nationwide Children's Hospital. See the article here. If you would like to contact us, please email Dr. Phiel at Christopher.Phiel@nationwidechildrens.org. Or call at 614 722-5916. Funding provided by NIH/NIA (R01) AG031883.
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2 personnel
3 protocols
4 reagents
5 publications
6 links
7 about us
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the Phiel Lab | phiellab.com Reviews

https://phiellab.com

Second, we are interested in understanding the mechanism of the ketogenic diet. Which is used to treat epilepsy in children whose seizures are not controlled by drugs. Through an active collaboration with the Division of Neurology and the Ketogenic Clinic. Here at Nationwide Children's Hospital. See the article here. If you would like to contact us, please email Dr. Phiel at Christopher.Phiel@nationwidechildrens.org. Or call at 614 722-5916. Funding provided by NIH/NIA (R01) AG031883.

INTERNAL PAGES

phiellab.com phiellab.com
1

the Phiel Lab

http://www.phiellab.com/personnel.html

We are a group of graduate students and laboratory technicians interested in investigating this thing called science. The current members of our lab include:. Christopher Phiel, PI.

2

the Phiel Lab

http://www.phiellab.com/protocols.html

We work with a variety of experimental procedures from southern blotting to mammalian cell culture and have posted here what works for us. Feel free to download and use any of our protocols and don't hesitate to contact us with any questions. Bisulfite Conversion of Genomic DNA - we have used both the Invitrogen MethylCode. And the Zymo Research EZ DNA Methylation. Primer Design Strategy for Bisulfite Sequencing. Sequencing of Bisulfite Converted DNA - coming soon. Website for more information.

3

the Phiel Lab

http://www.phiellab.com/links.html

Halloween at TRINCH - 2009. Halloween at CCRI - 2008. ICAD 2008 - Chicago, Illinois. Wnt Meeting 2008 - Keystone, Colorado. Harvard PlasmID DF/HCC DNA Resource Core. Offers numerous genes in Donor vectors for easy LR Gateway cloning into your destination vector of choice. A good resource for locating plasmids.

4

the Phiel Lab

http://www.phiellab.com/reagents.html

FL GSK-3α ORF. GSK-3α ΔNT. GSK-3α ΔCT1. GSK-3α ΔCT2. GSK-3α ΔCT3. GSK-3α ΔCT4. FL GSK-3β ORF. GSK-3β ΔNT. GSK-3β ΔCT1. GSK-3β ΔCT2. GSK-3β ΔCT3. GSK-3β ΔCT4. Dnmt3a2 WT promoter pCR8GW. Dnmt3a2 NMyc mut promoter pCR8GW. Dnmt3a2 WT promoter pGLF. Dnmt3a2 NMyc mut promoter pGLF. DEST47 (C-terminal GFP tag). DEST53 (N-terminal GFP tag). 26 (N-terminal 6xHis tag). 27 (N-terminal GST tag). DEST40 (C-terminal V5 6xHis tag). PCAGEN-GW (chicken β-actin promoter, untagged).

5

the Phiel Lab

http://www.phiellab.com/index.html

Second, we are interested in understanding the mechanism of the ketogenic diet. Which is used to treat epilepsy in children whose seizures are not controlled by drugs. Through an active collaboration with the Division of Neurology and the Ketogenic Clinic. Here at Nationwide Children's Hospital. See the article here. If you would like to contact us, please email Dr. Phiel at Christopher.Phiel@nationwidechildrens.org. Or call at 614 722-5916. Funding provided by NIH/NIA (R01) AG031883.

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