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Ion channels assays patch clamp. Action potential intracellular microelectrode. PhysioStim was created in 2000. On a sun- kissed ground where grows the pink garlic. They were only two at that time, now we are more than 11. Everything began in 2000. With the idea to raise a challenge: become the CRO. Independent, specialized in the field of the cardiac electrophysiology. Fifteen years later, PhysioStim is a niche CRO. Recognized as such through its expertise, its motivation and the loyalty of its customers.

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Ion channels assays patch clamp. Action potential intracellular microelectrode. PhysioStim was created in 2000. On a sun- kissed ground where grows the pink garlic. They were only two at that time, now we are more than 11. Everything began in 2000. With the idea to raise a challenge: become the CRO. Independent, specialized in the field of the cardiac electrophysiology. Fifteen years later, PhysioStim is a niche CRO. Recognized as such through its expertise, its motivation and the loyalty of its customers.

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1 start here

2 about us

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6 quality assurance

7 expertise

8 preclinical services

9 herg assay

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start here,about us,overview,management,objectives,quality assurance,expertise,preclinical services,herg assay,hnav 1,hkvlqt 1,mink,hcav 1,herg trafficking,isolated cardiomyocytes,human cardiomyocytes,guinea pig cardiomyocytes,purkinje fibres,rabbit atria

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Ion channels assays patch clamp. Action potential intracellular microelectrode. PhysioStim was created in 2000. On a sun- kissed ground where grows the pink garlic. They were only two at that time, now we are more than 11. Everything began in 2000. With the idea to raise a challenge: become the CRO. Independent, specialized in the field of the cardiac electrophysiology. Fifteen years later, PhysioStim is a niche CRO. Recognized as such through its expertise, its motivation and the loyalty of its customers.

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Human cardiomyocytes

http://www.physiostim.com/index.php/en/service-2/isolated-cardiomyocytes/human-cardiomyocytes

Ion channels assays patch clamp. Action potential intracellular microelectrode. Human cardiomyocytes derived from iPS cells. Models for drug development. Action Potential Duration at 20. MV), Amplitude (mV), APD. Voltage clamp: specific currents recorded with specific inhibitors. Reference compounds or positive controls. Developped by Pcie .fr.

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hERG Trafficking

http://www.physiostim.com/index.php/en/service-2/patch-clamp-2/inhibition-trafficking

Ion channels assays patch clamp. Action potential intracellular microelectrode. HERG trafficking inhibition on Human Embryonic Kidney ( HEK. Long term exposure of drugs to ion channel can lead to disruption of the trafficking of the ion channel to the cell surface membrane. It has been clearly demonstrated with the hERG channel where many of the LQT 2. Amplitude of the tail current upon repolarization from 70. Amplitude of the base current at 80. Inhibition of hERG tail current amplitude (%).

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Rabbit Atria

http://www.physiostim.com/index.php/en/service-2/microelectrode-2/rabbit-atria

Ion channels assays patch clamp. Action potential intracellular microelectrode. Standard Protocol for GLP. Concentrations of assay compound. Washout period or positive control at the end of the experiment. Conduction time (Ct) (ms). Conduction speed (Cs) (m/ s). Action Potential Duration at 50. Of repolarization (ms) = APD. Treated groups could be compared to a control vehicle group performed in the same conditions. External hypokaliemic conditions, chronic pre- treatment, incremental stimulation rate.

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Guinea-Pig cardiomyocytes

http://www.physiostim.com/index.php/en/service-2/isolated-cardiomyocytes/guinea-pig-cardiomyocytes

Ion channels assays patch clamp. Action potential intracellular microelectrode. Guinea- pig ventricular cardiomyocytes are isolated by enzymatic digestion. This model allows to reduce the number of animals (one animal can provide several experiments. Action Potential Duration at 20. Reference compounds or positive controls. Developped by Pcie .fr.

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Action potential intracellular microelectrode

http://www.physiostim.com/index.php/en/service-2/microelectrode-2

Ion channels assays patch clamp. Action potential intracellular microelectrode. The action potential is the consequence of the movements of ions through the different transmembrane ionic channels. Therefore, recording cardiac action potentials, using the microelectrode technique, allows to investigate the repercussion of mix ion channels effects. On cardiac electrical activity during early cardiac safety assessment. Rabbit and dog Purkinje fibres. Guinea pig papillary muscles. Developped by Pcie .fr.

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Ion channels assays patch clamp. Action potential intracellular microelectrode. PhysioStim was created in 2000. On a sun- kissed ground where grows the pink garlic. They were only two at that time, now we are more than 11. Everything began in 2000. With the idea to raise a challenge: become the CRO. Independent, specialized in the field of the cardiac electrophysiology. Fifteen years later, PhysioStim is a niche CRO. Recognized as such through its expertise, its motivation and the loyalty of its customers.

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