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Integrative Plasticity

Integrative analysis of plasticity in cell fate determination in plants. Species to be Studied:. NSF Plant Genome Research Program. From the germination of a seed to the fertilization events that lead to the next generation, plant development is exquisitely orchestrated by genetically determined processes that are fine-tuned by environmental cues. This entails the precise regulation of networks of genes in individual cells over the course of the plant life cycle. Practical Applications of Research. We ar...

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Integrative Plasticity | plant-plasticity.github.io Reviews

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Integrative analysis of plasticity in cell fate determination in plants. Species to be Studied:. NSF Plant Genome Research Program. From the germination of a seed to the fertilization events that lead to the next generation, plant development is exquisitely orchestrated by genetically determined processes that are fine-tuned by environmental cues. This entails the precise regulation of networks of genes in individual cells over the course of the plant life cycle. Practical Applications of Research. We ar...

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1 integrative plasticity

2 aims

3 outreach

4 software

5 protocols

6 people

7 publications

8 oryza sativa

9 solanum lycopersicum

10 s pennellii

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integrative plasticity,aims,outreach,software,protocols,people,publications,oryza sativa,solanum lycopersicum,s pennellii,medicago truncatula,funded by,and gramene,experimental approaches,mrna,nuclear transcriptome,histone modifications,epigenome

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Integrative Plasticity | plant-plasticity.github.io Reviews

https://plant-plasticity.github.io

Integrative analysis of plasticity in cell fate determination in plants. Species to be Studied:. NSF Plant Genome Research Program. From the germination of a seed to the fertilization events that lead to the next generation, plant development is exquisitely orchestrated by genetically determined processes that are fine-tuned by environmental cues. This entails the precise regulation of networks of genes in individual cells over the course of the plant life cycle. Practical Applications of Research. We ar...

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plant-plasticity.github.io

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Integrative Plasticity

http://plant-plasticity.github.io/protocols

INTACT and TRAP vectors:. Strain ATCC15834 from ATCC. For CRISPR/Cas9 we recommend using the vectors published by the Puchta lab. INTACT (Isolation of Nuclei TAgged in specific Cell Types). Download: INTACT protocol (PDF). TRAP (Translating Ribosome Affinity Purification). Download: Trap protocol (PDF).

2

Integrative Plasticity

http://plant-plasticity.github.io/software

Pipelines for RNA-Seq analysis. Pipelines for ChIP-Seq analysis.

3

Integrative Plasticity

http://plant-plasticity.github.io/outreach

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Integrative Plasticity

http://plant-plasticity.github.io/publications

Covington MF, Ichihashi Y, Zumstein K, Sinha NR (2015). BrAD-seq: Breath Adapter Directional sequencing: a streamlined, ultra-simple and fast library preparation protocol for strand specific mRNA library construction. Front Plant Sci 6: 366. Hasegawa PM, Bailey-Serres J (2015). Genetic mechanisms of abiotic stress tolerance that translate to crop yield stability. Nat Rev Genet 16(4): 237-51. Bailey-Serres J (2015). Flood adaptive traits and processes: an overview. New Phytol 206(1): 57-73.

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Integrative Plasticity

http://plant-plasticity.github.io/aims

Aim 1: Establish INTACT and TRAP tools in tomato, rice and. For comparison of the epigenome, transcriptome, and translatome for root and shoot meristem tissues. We will produce genotypes for INTACT and TRAP targeted to actively dividing cells and the functional shoot and root apical meristems. With these we will compare the growth apices of different species using multiple genome scale readouts including the nuclear epigenome (H3K27me3 and H3K4me3), transcriptome, and translatome. We are already identify...

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plant-plasticity.github.io

Integrative Plasticity

Integrative analysis of plasticity in cell fate determination in plants. Species to be Studied:. NSF Plant Genome Research Program. From the germination of a seed to the fertilization events that lead to the next generation, plant development is exquisitely orchestrated by genetically determined processes that are fine-tuned by environmental cues. This entails the precise regulation of networks of genes in individual cells over the course of the plant life cycle. Practical Applications of Research. We ar...

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