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Plasmid Project

Tuesday, December 18, 2007. PGLO plasmid cultures, 32C incubation and mixing. GFP proteins purified with HIC chromatography. Wednesday, November 28, 2007. Got 2 tubes, labeled /- pGLO. Placed in foam tube rack. Scooped one colony of E. Coli from starter plate. Inserted into tubes and spun. Put pGLO plasmids into pGLO tube. Put tubes in ice for 10 min. PGLO= LB/amp, LB/amp/ara. PGLO= LB, LB/amp. Heat shocked tubes, 0 C ice, 42 C water (50 sec.), 0 C ice (2 min.). Changed to dryer foam rack. Worried about ...

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Plasmid Project | plasmid-science-fair.blogspot.com Reviews
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Tuesday, December 18, 2007. PGLO plasmid cultures, 32C incubation and mixing. GFP proteins purified with HIC chromatography. Wednesday, November 28, 2007. Got 2 tubes, labeled /- pGLO. Placed in foam tube rack. Scooped one colony of E. Coli from starter plate. Inserted into tubes and spun. Put pGLO plasmids into pGLO tube. Put tubes in ice for 10 min. PGLO= LB/amp, LB/amp/ara. PGLO= LB, LB/amp. Heat shocked tubes, 0 C ice, 42 C water (50 sec.), 0 C ice (2 min.). Changed to dryer foam rack. Worried about ...
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1 plasmid project
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3 gangjutius
4 1 comment
5 no comments
6 lab notebook 3
7 inserted transformation solution
8 placed on ice
9 labeled agar plates
10 wait 10 min
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plasmid project,posted by,gangjutius,1 comment,no comments,lab notebook 3,inserted transformation solution,placed on ice,labeled agar plates,wait 10 min,insert lb broth,put into incubator,make duplicate,note,observations,trial 1,pglo,lab notebook 2,yeast
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Plasmid Project | plasmid-science-fair.blogspot.com Reviews

https://plasmid-science-fair.blogspot.com

Tuesday, December 18, 2007. PGLO plasmid cultures, 32C incubation and mixing. GFP proteins purified with HIC chromatography. Wednesday, November 28, 2007. Got 2 tubes, labeled /- pGLO. Placed in foam tube rack. Scooped one colony of E. Coli from starter plate. Inserted into tubes and spun. Put pGLO plasmids into pGLO tube. Put tubes in ice for 10 min. PGLO= LB/amp, LB/amp/ara. PGLO= LB, LB/amp. Heat shocked tubes, 0 C ice, 42 C water (50 sec.), 0 C ice (2 min.). Changed to dryer foam rack. Worried about ...

INTERNAL PAGES

plasmid-science-fair.blogspot.com plasmid-science-fair.blogspot.com
1

Plasmid Project: December 2007

http://plasmid-science-fair.blogspot.com/2007_12_01_archive.html

Tuesday, December 18, 2007. PGLO plasmid cultures, 32C incubation and mixing. GFP proteins purified with HIC chromatography. Subscribe to: Posts (Atom). PGLO plasmid cultures, 32C incubation and mixing. GFP proteins purified with HIC chromatography. Just a guy working on some projects, writing, and random stuff while I get through school. View my complete profile.

2

Plasmid Project: GFP proteins purified with HIC chromatography

http://plasmid-science-fair.blogspot.com/2007/12/gfp-proteins-purified-with-hic.html

Tuesday, December 18, 2007. GFP proteins purified with HIC chromatography. Subscribe to: Post Comments (Atom). PGLO plasmid cultures, 32C incubation and mixing. GFP proteins purified with HIC chromatography. Just a guy working on some projects, writing, and random stuff while I get through school. View my complete profile.

3

Plasmid Project: Incubator

http://plasmid-science-fair.blogspot.com/2007/11/incubator.html

Tuesday, November 13, 2007. Attempt 1- Used high power light bulb,aluminum foil. too hot, melted fish tank. Attempt 2- Used Christmas lights,towel. stays 40 C. maintains constant temperature. will download pictures. Light bulb- too large to fit, cannot spread heat all over area, only concentrates on one point. too hot, melted plastic. possibly unusable due to High and low temperature depending on area of tank. covered with aluminum. may have had some effect. Subscribe to: Post Comments (Atom).

4

Plasmid Project: October 2007

http://plasmid-science-fair.blogspot.com/2007_10_01_archive.html

Sunday, October 14, 2007. Bulletin 3052.pdf (application/pdf Object). Bulletin 3052.pdf (application/pdf Object). Research plan for project. Research Plan Attachment 2007-2008. TRANSFORMATION OF PLASMID pGLO IN E. COLI HB101. A Problem/Purpose/Question Being Addressed. In my Science Fair Project, I shall Transform foreign DNA, by inserting the DNA into bacteria, such as E. Coli. I will learn about E. Coli, DNA, and lab techniques that may help me in the future. The questions I am asking are:. I also beli...

5

Plasmid Project: Lab notebook

http://plasmid-science-fair.blogspot.com/2007/11/lab-notebook.html

Saturday, November 17, 2007. Added 500ml of distilled water to glass. added agar. Did not fit in microwave. changed to plastic container. Brought to boil in microwave 3 times. Labeled agar plates= 16 LB, 16 LB/amp, 8 LB/amp/ara, while agar solution cooled. Rehydhrated ampicillin and arabinose. may have added too much transformation solution to ampicillin. Poured agar solution on plates. Forgot to add ampicillin/arabinose, started over. may cause problems later. Have to re-order materials.

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Plasmid Project

Tuesday, December 18, 2007. PGLO plasmid cultures, 32C incubation and mixing. GFP proteins purified with HIC chromatography. Wednesday, November 28, 2007. Got 2 tubes, labeled /- pGLO. Placed in foam tube rack. Scooped one colony of E. Coli from starter plate. Inserted into tubes and spun. Put pGLO plasmids into pGLO tube. Put tubes in ice for 10 min. PGLO= LB/amp, LB/amp/ara. PGLO= LB, LB/amp. Heat shocked tubes, 0 C ice, 42 C water (50 sec.), 0 C ice (2 min.). Changed to dryer foam rack. Worried about ...

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