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Literature

http://www.preselector.org/index.php/literature

About restriction enzymes and their application:. Nathans D, Smith HO. Restriction endonucleases in the analysis and restructuring of dna molecules. Annu Rev Biochem. 1975;44:273-93. [NCBI]. About the restriction enzyme database used:. Roberts RJ, Vincze T, Posfai J, Macelis D. REBASE- a database for DNA restriction and modification: enzymes, genes and genomes. Nucleic Acids Res. 2010 Jan;38(Database issue):D234-6. doi: 10.1093/nar/gkp874. Epub 2009 Oct 21. [NCBI].

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About

http://www.preselector.org/index.php/about

This site was created by:. Martin Gühmann from the Max Planck Institute for Developmental Biology. And members of the Mrowka Lab at UKJ. For research use only. Not for diagnostic or therapeutic applications.

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Find Your Preselection

http://www.preselector.org/index.php/find-your-preselection

Input of first DNA sequence:. Or choose a file to upload:. Input of second DNA sequence:. Or choose a file to upload:.

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Documentation

http://www.preselector.org/index.php/documentation

In molecular cloning, restriction enzymes are used to cut out a fragment of DNA from a Donor Vector to ligate it then into a previously opened Recipient Vector. The DNA fragments are separated on an agarose gel. However, restriction enzymes may digest incompletely. Graphical definition of a typical preselection problem. What do you need to provide? You have to provide your two sequences in plain text or in fasta-format. You can copy and paste the sequences into our web form, or upload them as two fil...

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Results

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Considering commercial enzymes onlyThere was no input for sequence 1. There was no input for sequence 2. Please go to the "Find your Preselection" page.

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