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Science of Proteomics

About Proteomics, Mass Spectrometry, as well as Bioinformatics. Custom graphics for the node in cytoscape. How to custom your own graphics for the node in cytoscape? 随着Matthias mann的摇旗呐喊,Q-Exactive终于横空出世了. 怎么看这都是一个conspiracy,那边是ASMS的首发,这边是mcp文章的上线. 其实不是,这条路其实是弯着走过来的.与Q-TOF相比,Q-Exactive其实反而与Q-trap相似,反而结合了Q-trap与Q-tof的有点,又准,又快,而其还有类似的trap功能.从文章看,速度也还行,谁知道这是不是有一个疯狂竞争的对象? 原始链接 http:/ www.antpedia.com/viewspace-1447. 时，有几种方法可以激发离子，让其碎裂。最有效的和被最广泛地使用的是共振激发（. 左右），持续时间大概为几十个毫秒。 65289;。所以，选择在共振激发过程中的. 在线分析是在流动相以低流...

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Science of Proteomics | proteomics-ms.blogspot.com Reviews

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About Proteomics, Mass Spectrometry, as well as Bioinformatics. Custom graphics for the node in cytoscape. How to custom your own graphics for the node in cytoscape? 随着Matthias mann的摇旗呐喊,Q-Exactive终于横空出世了. 怎么看这都是一个conspiracy,那边是ASMS的首发,这边是mcp文章的上线. 其实不是,这条路其实是弯着走过来的.与Q-TOF相比,Q-Exactive其实反而与Q-trap相似,反而结合了Q-trap与Q-tof的有点,又准,又快,而其还有类似的trap功能.从文章看,速度也还行,谁知道这是不是有一个疯狂竞争的对象? 原始链接 http:/ www.antpedia.com/viewspace-1447. 时，有几种方法可以激发离子，让其碎裂。最有效的和被最广泛地使用的是共振激发（. 左右），持续时间大概为几十个毫秒。 65289;。所以，选择在共振激发过程中的. 在线分析是在流动相以低流...

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1 science of proteomics

2 like this one

3 congrates

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5 没有评论

6 q exactive横空出世

7 转载pqd

8 一个重要的能力是做

9 ms/ms

10 resonance excitation

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science of proteomics,like this one,congrates,big ben,没有评论,q exactive横空出世,转载pqd,一个重要的能力是做,ms/ms,resonance excitation,时，使用加在端盖电极上的,电压（注：,电压分两种，一个高幅的,电压会导致共振逐出，一个低幅的,电压会引起共振激发）共振激发的,电压一般幅度很小（,的频率需和离子的运动频率匹配；而离子的运动频率和主要的,trapping,场的幅度和离子的,当离子的振动频率与,值正比于

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Science of Proteomics | proteomics-ms.blogspot.com Reviews

https://proteomics-ms.blogspot.com

About Proteomics, Mass Spectrometry, as well as Bioinformatics. Custom graphics for the node in cytoscape. How to custom your own graphics for the node in cytoscape? 随着Matthias mann的摇旗呐喊,Q-Exactive终于横空出世了. 怎么看这都是一个conspiracy,那边是ASMS的首发,这边是mcp文章的上线. 其实不是,这条路其实是弯着走过来的.与Q-TOF相比,Q-Exactive其实反而与Q-trap相似,反而结合了Q-trap与Q-tof的有点,又准,又快,而其还有类似的trap功能.从文章看,速度也还行,谁知道这是不是有一个疯狂竞争的对象? 原始链接 http:/ www.antpedia.com/viewspace-1447. 时，有几种方法可以激发离子，让其碎裂。最有效的和被最广泛地使用的是共振激发（. 左右），持续时间大概为几十个毫秒。 65289;。所以，选择在共振激发过程中的. 在线分析是在流动相以低流...

INTERNAL PAGES

proteomics-ms.blogspot.com

1

Science of Proteomics: 关于proteotypic peptide

http://proteomics-ms.blogspot.com/2008/12/proteotypic-peptide.html

About Proteomics, Mass Spectrometry, as well as Bioinformatics. 以前我们在进行prototypic peptide 的预测是时是否忽略了一些条件，比如在色谱水平上的典型性？订阅：帖子评论 (Atom). R self-made function histlines. R self-made function histbars. Awesome Inc.模板. 由 Blogger.

2

Science of Proteomics: Q-Exactive横空出世

http://proteomics-ms.blogspot.com/2011/06/q-exactive.html

About Proteomics, Mass Spectrometry, as well as Bioinformatics. 随着Matthias mann的摇旗呐喊,Q-Exactive终于横空出世了. 怎么看这都是一个conspiracy,那边是ASMS的首发,这边是mcp文章的上线. 其实不是,这条路其实是弯着走过来的.与Q-TOF相比,Q-Exactive其实反而与Q-trap相似,反而结合了Q-trap与Q-tof的有点,又准,又快,而其还有类似的trap功能.从文章看,速度也还行,谁知道这是不是有一个疯狂竞争的对象? 订阅：帖子评论 (Atom). Awesome Inc.模板. 由 Blogger.

3

Science of Proteomics: 十月 2008

http://proteomics-ms.blogspot.com/2008_10_01_archive.html

About Proteomics, Mass Spectrometry, as well as Bioinformatics. Perl script: substitute the a character at defined position in a string. Perl script：count the number of certain character. Site=($str= y/a/ ) # y/ / can be replaced by tr/ /. Print $site # contains the number of "a" in $str. Name line=(split/ /,$ );. Name=$name line[0]; #提取fasta条目索引，可能依赖于数据库格式. Name= s/ / ;. Chomp $ ;. If(m/ / or eof){. Sequence=$sequence.$ ;. 160;文献中常用noredundant 这个词，我们实验室也使用它来表示在蛋白质质谱鉴定的结果。 1、peptide 到 protein. 在质谱分析PTM...

4

Science of Proteomics: 转载：如何正确选择电喷雾针头

http://proteomics-ms.blogspot.com/2009/03/blog-post.html

About Proteomics, Mass Spectrometry, as well as Bioinformatics. Http:/ bbs.innosep.cn/thread-690-1-1.html. 在线分析是在流动相以低流速泵入，通过小直径电喷针进入质谱时，将高电压施加于流动相以产生均匀喷雾进入质谱仪。流动相的来源，最常见的是注射泵或液相色谱(LC),使样品通过色谱介质。离线分析是通过注射器或加样枪将已分离好的样品载入固定在质谱仪源上的针头上。产生样品流动离线技术是依赖于电喷针内部的液体静电作用，针头已施加电压，朝电极相反方向。 10 至 80 nL/min. 典型 25 nL/min ). 2 - 50 μm. 100 nL 至 1 μL/min. 典型 50 - 500 nL/min ). 固定在仪器上的电喷针必须适应150 或 360 μm 外径 (OD) 石英毛细管。常用360 μmOD是因为更容易处理并且可以有较宽范围ID规格和样式。标准电喷针长度是5 cm的 SilicaTips 和 50 cm的 TaperTips. 通过您的纳升级喷雾源的制造商来确定电喷针的适当OD...

5

Science of Proteomics: PQD

http://proteomics-ms.blogspot.com/2009/03/pqd.html

About Proteomics, Mass Spectrometry, as well as Bioinformatics. 原始链接 http:/ www.antpedia.com/viewspace-1447. 时，有几种方法可以激发离子，让其碎裂。最有效的和被最广泛地使用的是共振激发（. 左右），持续时间大概为几十个毫秒。电压上吸收能量，振幅加大，在一个理想的四极场中，如持续施加共振电压，离子的振幅将随时间线性增大。离子的动能随振幅平方的增大而增大，因此离子会和中性气体碰撞，使化学键断裂，获得. 12290;设置比较好的参数，才能获得充分的、信息量丰富的. 时，离子会在不稳定的轨道上，不能被捕获，即. 65289;。所以，选择在共振激发过程中的. 65289;离子的振幅，因为振幅最终决定了碰撞的动能，决定了是否可以形成丰富的. 值更高，离子动能更高，会更充分地碎裂，但是，同时，也提高了. 65292;导致更多的小质量碎片不能被观测到。因此，. 以下的碎片离子看不到。（（. 值，看到更小的碎片，但同时可能会损害碎裂效率。相似的，我们也可以增大. 订阅：帖子评论 (Atom).

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Science of Proteomics

About Proteomics, Mass Spectrometry, as well as Bioinformatics. Custom graphics for the node in cytoscape. How to custom your own graphics for the node in cytoscape? 随着Matthias mann的摇旗呐喊,Q-Exactive终于横空出世了. 怎么看这都是一个conspiracy,那边是ASMS的首发,这边是mcp文章的上线. 其实不是,这条路其实是弯着走过来的.与Q-TOF相比,Q-Exactive其实反而与Q-trap相似,反而结合了Q-trap与Q-tof的有点,又准,又快,而其还有类似的trap功能.从文章看,速度也还行,谁知道这是不是有一个疯狂竞争的对象? 原始链接 http:/ www.antpedia.com/viewspace-1447. 时，有几种方法可以激发离子，让其碎裂。最有效的和被最广泛地使用的是共振激发（. 左右），持续时间大概为几十个毫秒。 65289;。所以，选择在共振激发过程中的. 在线分析是在流动相以低流...

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