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Development of a new diagnostic tool using DNA barcoding to identify quarantine organisms in support of plant health

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PAGE TITLE
QBOL | qbol.org Reviews
<META>
DESCRIPTION
Development of a new diagnostic tool using DNA barcoding to identify quarantine organisms in support of plant health
<META>
KEYWORDS
1 areas of expertise
2 barcoding of arthropods
3 barcoding of bacteria
4 barcoding of fungi
5 barcoding of nematodes
6 barcoding of phytoplasmas
7 barcoding of viruses
8 dna banks
9 library/database/informatics
10 validation/evaluation
CONTENT
Page content here
KEYWORDS ON
PAGE
areas of expertise,barcoding of arthropods,barcoding of bacteria,barcoding of fungi,barcoding of nematodes,barcoding of phytoplasmas,barcoding of viruses,dna banks,library/database/informatics,validation/evaluation,dissemination,continue to,about qbol
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QBOL | qbol.org Reviews

https://qbol.org

Development of a new diagnostic tool using DNA barcoding to identify quarantine organisms in support of plant health

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qbol.org qbol.org
1

DNA barcode library / Database / Informatics

http://www.qbol.org/en/qbol/Activities/LibraryDatabaseInformatics.htm

DNA barcode library / Database / Informatics. The database (developed within the Dutch FES project, 2006-2010) has been further developed during the QBOL project in this work package. To create a complex database structure including and interlinking the different organisms (fungi, arthropods, bacteria, nematodes, viruses and phytoplasmas) and associated data. Task 9.1 Selection of fields to be included into the database for each group of organisms and associated functions and algorithms. D 92 Prototype o...

2

Barcoding of Viruses

http://www.qbol.org/en/qbol/Activities/Barcoding-of-Viruses.htm

Since viruses don’t contain a generic barcode gene, it was decided to sequence the whole genome of viruses in this work package using Next Generation Sequence Technology. The objective of this WP is to develop a DNA barcoding method for identification of high priority viruses present in the EU Plant Health Directive and EPPO Plant Health lists of quarantine pathogens. In order to achieve this aim we will:. Develop reliable nucleic acid (DNA&RNA) extraction procedures. Task 6.2 Nucleic acid extraction.

3

Q-BOL Activities

http://www.qbol.org/en/qbol/Activities.htm

Assemble all relevant collections of quarantine organisms available in EU and third countries and make them available. Investigate which and how many genes are required to identify quarantine organisms of each type (fungi, arthropods, bacteria, nematodes, viruses and phytoplasmas) using barcode sequences and generate these barcodes for the relevant taxa. The QBOL project is divided into several activities which are being coordinated by the QBOL consortium partners:. In this work package the work focussed...

4

About DNA Barcoding

http://www.qbol.org/en/qbol/DNA-Barcoding.htm

Development of accurate identification tools for plant pathogens and pests is vital to support European Plant Health Policies. The number of plants being traded worldwide increases, so the chance of harmful organisms spreading is enhanced. At the same time, the taxonomic knowledge available to identify harmful quarantine organisms via their visual characteristics is gradually decreasing. DNA barcoding has arisen as a robust and standardized approach to species identification. QBOL will make DNA barco...

5

DNA Banks

http://www.qbol.org/en/qbol/Activities/DNA-Banks.htm

DNA of quarantine organisms is scarce. Therefore in this work package an investigation in different protocols to store, transport and multiply DNA samples of these organisms was made. To develop protocols for the correct storage and whole genome amplification (WGA) of extracted DNA and RNA samples. To develop a DNA bank, containing DNA/RNA and/or WGA amplified material from selected quarantine and regulated plant pathogens and their taxonomically closely related species. Task 8.5 Contingency plan. DNA of...

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ecbol.org ecbol.org

ECBOL.org : European Consortium for the Barcode Of Life

http://www.ecbol.org/ECBOL.org/european-campaigns.html

What is ECBOL.org? What is DNA Barcoding? What are people doing? How can I get involved? Calibrating Europe's Biodiversity using DNA Barcodes. Interview Professor Pedro Crous. How do I start DNA barcoding? Workshop and Meeting Reports. Barcode of Life Data Systems (BOLD). Barcode of Life NL (NBOL). Catalogue of Life (Species 2000). Consortium for the Barcode of Life (CBOL). Canadian Barcode of Life Network (BOLNET). Canadian Centre for DNA Barcoding. Global Biodiversity Information Facility (GBIF).

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Links

http://www.ipwgnet.org/links

19 ICVG Conference - 2018 - Santiago, Chile. 24 ICVF Conference - June 5-9, 2017 - Thessaloniki, Greece. January 14-17, 2015. The abstract book of the meeting. Third IPWG Meeting - Phytopathogenic Mollicutes Vol. 5 (1-Supplement), January 2015. January 14-17, 2015. Thanks to all participants for the fruitful participation to the meeting. Meeting pictures are available online: click here. We have 5 guests online. VI Italian Meeting on Phytoplasmas and Phytoplasma Diseases web site.

costphytoplasma.ipwgnet.org costphytoplasma.ipwgnet.org

links

http://www.costphytoplasma.ipwgnet.org/links.htm

Cost web site Action FA0807. Cost action FA1003 GRAPENET. Information site Apple witches' broom disease (in German and French) Apfeltriebsucht / Prolifération du pommier. International Phytoplasmologist Working Group (IPWG). International Organization for Mycoplasmology (IOM). Leafhopper, Planthopper and Psyllid Vectors of Plant Diseases (National Museum Wales). Phytoplasma Web site (University of Tokyo). Pictorial key of Central European Cacopsylla species associated with Rosaceae.

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QBOL

Development of a new diagnostic tool using DNA barcoding to identify quarantine organisms in support of plant health. QBOL project has come to an end. On 21 September 2012 the QBOL project ended officially. Many results were obtained in the different work packages. A consortium of 20 partners. Universities, research institutes and phytosanitary organizations) from all over the world working together and sharing their research expertise in the field of DNA barcoding of Arthropods.

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