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Design - Analyze - Experiment - Play. Welcome to the qPCR Design Lab. Make sure to check back regularly as we will be updating this site with the latest information about real-time PCR. If you have any feedback or suggestions for this website, please contact qPCRdesign@biosearchtech.com. Learn about the history of PCR and the evolution of quencher chemistry and probe-based qPCR. Also review the current applications and numerous fields in which BHQ probes and BHQplus probes are used. Take a break and blow...

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qPCR Design Lab | Home | qpcrdesign.com Reviews

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Design - Analyze - Experiment - Play. Welcome to the qPCR Design Lab. Make sure to check back regularly as we will be updating this site with the latest information about real-time PCR. If you have any feedback or suggestions for this website, please contact qPCRdesign@biosearchtech.com. Learn about the history of PCR and the evolution of quencher chemistry and probe-based qPCR. Also review the current applications and numerous fields in which BHQ probes and BHQplus probes are used. Take a break and blow...

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qPCR Design Lab | Home | qpcrdesign.com Reviews

https://qpcrdesign.com

Design - Analyze - Experiment - Play. Welcome to the qPCR Design Lab. Make sure to check back regularly as we will be updating this site with the latest information about real-time PCR. If you have any feedback or suggestions for this website, please contact qPCRdesign@biosearchtech.com. Learn about the history of PCR and the evolution of quencher chemistry and probe-based qPCR. Also review the current applications and numerous fields in which BHQ probes and BHQplus probes are used. Take a break and blow...

INTERNAL PAGES

qpcrdesign.com

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qPCR Design Lab | qPCR Papers for Reference

http://www.qpcrdesign.com/qpcr-library

Get the qPCR references you need and stand on the shoulders of giants. Reporting and Methods for qPCR:. Citation: The real-time polymerase chain reaction. Kubista M, Andrade JM, Bengtsson M, Forootan A, Jonák J, Lind K, Sindelka, R, Sjöback R, Sjögreen B, Strömbom L, Ståhlberg A, Zoric N. Mol Aspects Med. 2006 27: 95-125. Citation: Quantification of mRNA using real-time RT-PCR. Nolan T, Hands RE, and Bustin SA. Nat Protoc. 2006;1(3):1559-82. Citation: Real-time PCR for mRNA quantitation. Citation: Detect...

2

qPCR Design Lab | qPCR Videos, Webinars & Animations

http://www.qpcrdesign.com/qpcr-videos

Sit back, relax and watch videos, animations, and webinars, all related to qPCR. Video - 30 Years of PCR History. From its birth Polymerase Chain Reaction, PCR, changed the molecular biology world. While working at Cetus Corporation, Kary Mullis uses a SAM I DNA synthesizer from Biosearch Technologies to test his hypothesis, and the rest, as they say, is history. Webinar - PCR: Past, Present and Future. Webinar - qPCR Assay Design 101. Video - Real-time PCR Probe Animation.

3

qPCR Design Lab | Oligo Toolbox for Quick Calculations

http://www.qpcrdesign.com/oligo-toolbox

The OligoSpec™ calculator provides the physical properties for your oligo design. Quickly calculate your oligo’s molecular weight and extinction coefficient. Enter some basic information about your reaction conditions to find out the number of reactions your amount of oligo can potentially perform. Easily create a stock solution by allowing the resuspension calculator take the guesswork out of dissolving your oligo. Spectral Overlay Tool for Multiplexed qPCR.

4

qPCR Design Lab | qPCR & BHQ Probe History

http://www.qpcrdesign.com/qpcr-bhq

Explore the story of PCR, from the first bands visualized on a gel to the sophisticated molecular diagnostics practiced today. Image of Biosearch’s SAM I, the first commercially successful solid phase DNA synthesizer. The automation of oligo synthesis improved the speed and efficiency of creating synthetic DNA for newly developed genetic methods such as PCR. Click here to download the PCR 30 poster. Chemical structures of BHQ-1 (left) and BHQ-2 (right) dyes. The excellent quenching efficiency of BHQ dyes...

5

qPCR Design Lab | Play qPCR Crush Game - Read Blog

http://www.qpcrdesign.com/qpcr-crush

No Work and All Play. QPCR Crush™ Game. Take some R&R from PCR by playing our qPCR Crush Game and see how many amplicons you can rack up in a minute! Top scorers have the choice to enter a monthly raffle for free Biosearch schwag and product discounts. Monthly Raffle Prize Winners.

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404 | LGC Biosearch Technologies

https://www.biosearchtech.com/customoligos

QPCR and SNP Genotyping. GMP and Commercial Services. OEM and Kit Manufacturing. 404 Page not found. We apologize for the inconvenience. The page you are looking for appears to have been moved, deleted, or does not exist. QPCR and SNP Genotyping. 1800Genome1 / 1.415.883.8400. 2199 South McDowell Blvd. Create a New Account. Invalid email address or password. Running low on your supply? Find your previously purchased item and order it again. Plan on ordering many oligo sequences at once? What is your name?

biosearchtech.com

404 | LGC Biosearch Technologies

https://www.biosearchtech.com/products/fluorogenic-probes-and-primers/superroxÂ®-reference-dye

QPCR and SNP Genotyping. GMP and Commercial Services. OEM and Kit Manufacturing. 404 Page not found. We apologize for the inconvenience. The page you are looking for appears to have been moved, deleted, or does not exist. QPCR and SNP Genotyping. 1800Genome1 / 1.415.883.8400. 2199 South McDowell Blvd. Create a New Account. Invalid email address or password. Running low on your supply? Find your previously purchased item and order it again. Plan on ordering many oligo sequences at once? What is your name?

biosearchtech.com

Molecular Beacons | LGC Biosearch Technologies

https://www.biosearchtech.com/molecularbeacons

QPCR and SNP Genotyping. GMP and Commercial Services. OEM and Kit Manufacturing. QPCR and SNP Genotyping. Molecular Beacons are a type dual-labeled FRET probes incorporating a quencher and a fluorophore reporter molecule. They differ from traditional dual-labeled probes, due to the incorporation of a short ( 5 b.p.) complementary stem sequence at the 3 and 5 ends. Just like standard Dual-labeled BHQ. Absorption and Emission Spectral Charts. Absorption Spectrum for BHQ-1 Dye. Absorption and Emission Spect...

biosearchtech.com

Synthesis Reagents | LGC Biosearch Technologies

https://www.biosearchtech.com/products/dnarna-synthesis-reagents.aspx

QPCR and SNP Genotyping. GMP and Commercial Services. OEM and Kit Manufacturing. We offer an extensive range of products for DNA and RNA synthesis including: synthesis supports, columns for 3 labeling and specialty amidites. These products are available for all of our fluorophores and quenchers as well as for many non-fluorescent labels. Synthesis and Purification Accessories. Carboxylic Acid, Succinimidyl Esters, and Amines. For synthesizing DNA or RNA we offer an extensive range of controlled pore glas...

biosearchtech.com

Scorpions Primers | LGC Biosearch Technologies

https://www.biosearchtech.com/scorpions

QPCR and SNP Genotyping. GMP and Commercial Services. OEM and Kit Manufacturing. QPCR and SNP Genotyping. BHQ (Black Hole Quencher)-labeled Scorpions primers for PCR analysis are unlike dual-labeled probes and Molecular Beacons because they combine primer and probe in one molecule, with the primer at the 3 end and the probe contained within a hairpin-loop structure at the 5 end. Scorpions primers provide a number of important advantages over other PCR probe formats:. The target-specific PCR primer is cov...

biosearchtech.com

ValuPanel Reagents | LGC Biosearch Technologies

https://www.biosearchtech.com/products/valupanel-reagents

QPCR and SNP Genotyping. GMP and Commercial Services. OEM and Kit Manufacturing. QPCR and SNP Genotyping. ValuPanel Reagents are composed of pre-designed probes and primers that are intended to resolve the genetic signatures to of different pathogen strains. They are for Research Use Only (RUO), not for use in diagnostic procedures. Avian Influenza A (H7N9) ValuPanel Reagents. Influenza A Subtyping ValuPanel Reagents. 2009 H1N1 ValuPanel Reagents. PDM 2009 H1N1 ValuPanel Reagents. LGC Biosearch’s PDM 200...

biosearchtech.com

2009 H1N1 ValuPanel Reagents | LGC Biosearch Technologies

https://www.biosearchtech.com/swineflu

QPCR and SNP Genotyping. GMP and Commercial Services. OEM and Kit Manufacturing. QPCR and SNP Genotyping. 2009 H1N1 ValuPanel Reagents. 2009 H1N1 ValuPanel Reagents. Related News and Resources. Browse All 2009 H1N1 ValuPanel Reagents. The 2009 H1N1 (Novel Influenza A) ValuPanel™ Reagents* are part of LGC Biosearch Technologies’ ValuPanel line of detection reagents. ValuPanel Reagents are composed of probes and primers that resolve genetic signatures of different pathogen strains. Swine H1 Influenza A.

biosearchtech.com

BHQplus Probes | LGC Biosearch Technologies

https://www.biosearchtech.com/customsnpassays

QPCR and SNP Genotyping. GMP and Commercial Services. OEM and Kit Manufacturing. QPCR and SNP Genotyping. BHQplus probes are offered in two convenient deliveries. For BHQplus probes and primers delivered in individual tubes select just “BHQplus probes”, however if you would like the probes and primers blended into a single tube select “ValuMix Assay for SNP genotyping”. Please refer to the Product Listings tab to view all dye-quencher combinations and delivery quantities available. Shorter sequences offe...

biosearchtech.com

Products | LGC Biosearch Technologies

https://www.biosearchtech.com/products

QPCR and SNP Genotyping. GMP and Commercial Services. OEM and Kit Manufacturing. QPCR and SNP Genotyping. LGC Biosearch Technologies offers a variety of probe and primer types for real-time qPCR, including dual-labeled BHQ probes, BHQplus probes, Molecular Beacons, and Scorpions primers. Whether you require labeled or unlabeled oligos, we can meet your exact needs as we offer more custom modifications than any other oligo supplier at a cost well below the prevailing market rate. QPCR and SNP Genotyping.

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PhileKorea

http://www.philekorea.com/eng/index.php

Gel and PCR Purification. ECube Blood DNA Mini Kit. ECube Tissue DNA Mini Kit. ECube Blood DNA Maxi Kit. ECube Soil DNA Mini Kit. ECube Stool DNA Mini Kit. ECube Plant DNA Mini Kit. ECube Blood RNA Mini Kit. ECube Tissue RNA Mini Kit. ECube Plant RNA Mini Kit. ECube-Tri RNA Clean-up Kit. ECube miRNA Isolation Kit. ECube Viral Nucleic Acid Mini Kit. ECube Plasmid DNA Mini Kit. ECube Plasmid DNA Midi Kit. ECube Plasmid DNA Maxi Kit. ECube PCR Purification Kit. ECube Gel Purification Kit. QuantiSpeed Probe ...

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qPCR 2011 - Molecular Diagnostics: from single-cells to Next Generation Sequencing

QPCR 2011 - Molecular Diagnostics: from single-cells to Next Generation Sequencing - Main topic: Molecular diagnostics in single-cells Scientific Topics: Molecular diagnostics in single-cells, High throughput analysis in qPCR and NGS (Next Generation Sequencing), MIQE and QM strategies in qPCR, small RNAs qPCR - microRNA and siRNA Applications, digital PCR and Nano-fluidics, Pre-analytical Steps, PCR BioStatistics and BioInformatics - qpcr2011.net.

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qPCR Design Lab | Home

Design - Analyze - Experiment - Play. Welcome to the qPCR Design Lab. Make sure to check back regularly as we will be updating this site with the latest information about real-time PCR. If you have any feedback or suggestions for this website, please contact qPCRdesign@biosearchtech.com. Learn about the history of PCR and the evolution of quencher chemistry and probe-based qPCR. Also review the current applications and numerous fields in which BHQ probes and BHQplus probes are used. Take a break and blow...

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