rasmusfrandsen.dk

Home page of Rasmus Frandsen

Aim of the homepage. This homepage was primarily created to communicate information on Fusarium graminearum. Mutants (pheno- and chemotypes), fusaria metabolites and the technologies I have used to generate targeted gene replacement mutants. It is my hope that the page will aid in the understanding of fungal secondary metabolism and is indented to be read by both skilled professionals and people with a general interest in biology/biochemistry. Aim of my current project:. Biology and genetics of Fusarium.

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Home page of Rasmus Frandsen | rasmusfrandsen.dk Reviews

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Aim of the homepage. This homepage was primarily created to communicate information on Fusarium graminearum. Mutants (pheno- and chemotypes), fusaria metabolites and the technologies I have used to generate targeted gene replacement mutants. It is my hope that the page will aid in the understanding of fungal secondary metabolism and is indented to be read by both skilled professionals and people with a general interest in biology/biochemistry. Aim of my current project:. Biology and genetics of Fusarium.

<META> KEYWORDS

1 personer har

2 besøgt siden

3 a t bio dtu

4 current work place

5 research interests

6 agrobacterium

7 mediated transformation

8 user friendly cloning

9 polyketide synthases

10 learn more about

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personer har,besøgt siden,a t bio dtu,current work place,research interests,agrobacterium,mediated transformation,user friendly cloning,polyketide synthases,learn more about,theory,secondary metabolites,polyketide syntase,systems,aflatoxin,aurofusarin

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Home page of Rasmus Frandsen | rasmusfrandsen.dk Reviews

https://rasmusfrandsen.dk

Aim of the homepage. This homepage was primarily created to communicate information on Fusarium graminearum. Mutants (pheno- and chemotypes), fusaria metabolites and the technologies I have used to generate targeted gene replacement mutants. It is my hope that the page will aid in the understanding of fungal secondary metabolism and is indented to be read by both skilled professionals and people with a general interest in biology/biochemistry. Aim of my current project:. Biology and genetics of Fusarium.

INTERNAL PAGES

rasmusfrandsen.dk

1

Experimental setup

http://www.rasmusfrandsen.dk/experimental_setup.htm

The following section describes which primers you will need to design for performing targeted genome modifications (deletion and in locus. Overexpression) in fungi by USER Friendly cloning. You will need primers for 1) construction of the vector, 2) verification of the vector and 3) verification of the genetically modified strain. Instruction and tips for the three different primer types can be found in the following sections. Need to obtain the Agrobacterium tumefaciens. The overhangs, as described below.

2

ATMT of new fungi

http://www.rasmusfrandsen.dk/atmt_of_new_fungal_species.htm

When setting up Agrobacterium. Mediated transformation for a fungus for which no protocol exists, the following check list most likely will help the process. Test that the filter papers you plan to use does not inhibit fungal growth and that the fungus is able to grow though the filters and into the underlying solid medium. Many factors can affect the transformation frequency, ranging from co-cultivation period, temperature, Agrobacterium tumefaciens. And the fungal material you are trying to transform.

3

USER cloning

http://www.rasmusfrandsen.dk/user_cloning.htm

The USER Friendly technology. Standard restriction enzyme) and Nt.Bbc.CI. Annealing of the 9 bp long sequences in the PCR insert and the vector DNA is stable enough to survive chemical transformation into E. coli. Inside E. coli. The DNA fragments are covalently linked by the formation of phosphodiester bonds between the free DNA ends. Vector with the two new unique USER cloning sites, that each consists of a PacI. Site, two Nt.BbvC IB. The two vector fragments following the combined cutting of PacI.

4

Data

http://www.rasmusfrandsen.dk/feedback.htm

UV and VIS spectrums for selected Fusarium. Vectors compatible with USER Friendly cloning. Dette sted blev sidst opdateret 10. July 2010.

5

Aflatoxin

http://www.rasmusfrandsen.dk/aflatoxin.htm

The best described fungal polyketide gene cluster. The gene clusters responsible for the biosynthesis of polyketides in fungi typically consist of a PKS. Gene , genes encoding hydrogenases, oxidases, transporters and regulatory genes (Keller and Hohn 1997) (Yu et al. S aflatoxin and sterigmatocystin (Bhatnagar et al. 2003), AK-toxin (Tanaka et al. 1999), fumonisins (Bojja et al. HC-toxin (Ahn and Walton 1999), lovastatin (Kennedy et al. 1999), melanins (Tsai et al. 1999) and paxilline (Young et al. Until...

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