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RefGenes - condition specific reference genes for RT-qPCRNEBION
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RefGenes - condition specific reference genes for RT-qPCR | refgenes.org Reviews
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refgenes.org
RefGenes - condition specific reference genes for RT-qPCR
http://refgenes.org/rg/doc/copyright.jsp
The RefGenes website and the information contained and referenced therein are for informational purposes only. Any reproduction, retransmission or other use is strictly prohibited. To reproduce any information contained on this website, requests should be addressed to NEBION.
RefGenes - condition specific reference genes for RT-qPCR
http://refgenes.org/rg/index.jsp
2 Choose biological context similar to your RT-qPCR experiment. 3 Enter a target gene of interest to see its range of expression. 4 Search for genes highly stable in this biological context and in that range of expression. Cited RefGenes in one of your publications? And get a 6 months free access to GENEVESTIGATOR Professional!
RefGenes - condition specific reference genes for RT-qPCR
http://refgenes.org/rg/doc/news.jsp
The primary purpose of RefGenes is to provide candidate reference genes for particular contexts or tissues. Once a subset of genes is chosen, you still need to validate them within your own experiment, as you would do if you had started with genes from a housekeeping gene panel. Here are a few solution providers or tools that we recommend:. Designing and ordering primers: Primerdesign. Selection of most stable tested genes: genormPLUS. Software for qPCR analysis: qbasePLUS. See talk presentation: [PDF].
RefGenes - condition specific reference genes for RT-qPCR
http://refgenes.org/rg/doc/imprint.jsp
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RefGenes - condition specific reference genes for RT-qPCR
http://refgenes.org/rg/doc/example.jsp
Identifying reference genes for mouse liver. Preliminary remark: the below example was carried out with RefGenes and was experimentally validated. The results are published in the RefGenes article ([ PDF. Add new array selection. Select organism (in this case: mouse). Select platform (in this case: Affymetrix mouse 40k array). Select by annotations (under "Anatomy", select "Liver"). This resulted in a set of 197 microarrays (in the meantime, there are more than 600 mouse liver samples). In order to valid...
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Normalisation of real-time RT-PCR reactions
http://www.gene-quantification.org/hkg.html
Reference Genes / Housekeeping Genes (1). In real-time PCR - page 2. In real-time PCR - page 3. In real-time PCR - page 4. Data normalisation in real-time RT-PCR is a further major step in gene quantification analysis ( Bustin 2002. Any variation in the normalizer will obscure real changes and produce artifactual changes. And cDNA sample loading variation ( Stahlberg 2003. Pfaffl and Hageleit 2001). Or Ribogreen RNA Quantification Kit. Alternatively the rRNA content has been proposed as an optimal an...
Normalisation of real-time RT-PCR reactions
http://www.gene-quantification.net/hkg.html
Reference Genes / Housekeeping Genes (1). In real-time PCR - page 2. In real-time PCR - page 3. In real-time PCR - page 4. Data normalisation in real-time RT-PCR is a further major step in gene quantification analysis ( Bustin 2002. Any variation in the normalizer will obscure real changes and produce artifactual changes. And cDNA sample loading variation ( Stahlberg 2003. Pfaffl and Hageleit 2001). Or Ribogreen RNA Quantification Kit. Alternatively the rRNA content has been proposed as an optimal an...
Normalisation of real-time RT-PCR reactions
http://www.gene-quantification.com/hkg.html
Reference Genes / Housekeeping Genes (1). In real-time PCR - page 2. In real-time PCR - page 3. In real-time PCR - page 4. Data normalisation in real-time RT-PCR is a further major step in gene quantification analysis ( Bustin 2002. Any variation in the normalizer will obscure real changes and produce artifactual changes. And cDNA sample loading variation ( Stahlberg 2003. Pfaffl and Hageleit 2001). Or Ribogreen RNA Quantification Kit. Alternatively the rRNA content has been proposed as an optimal an...
Normalisation of real-time RT-PCR reactions
http://www.gene-quantification.biz/hkg.html
Reference Genes / Housekeeping Genes (1). In real-time PCR - page 2. In real-time PCR - page 3. In real-time PCR - page 4. Data normalisation in real-time RT-PCR is a further major step in gene quantification analysis ( Bustin 2002. Any variation in the normalizer will obscure real changes and produce artifactual changes. And cDNA sample loading variation ( Stahlberg 2003. Pfaffl and Hageleit 2001). Or Ribogreen RNA Quantification Kit. Alternatively the rRNA content has been proposed as an optimal an...
Normalisation of real-time RT-PCR reactions
http://www.gene-quantification.de/hkg.html
Reference Genes / Housekeeping Genes (1). In real-time PCR - page 2. In real-time PCR - page 3. In real-time PCR - page 4. Data normalisation in real-time RT-PCR is a further major step in gene quantification analysis ( Bustin 2002. Any variation in the normalizer will obscure real changes and produce artifactual changes. And cDNA sample loading variation ( Stahlberg 2003. Pfaffl and Hageleit 2001). Or Ribogreen RNA Quantification Kit. Alternatively the rRNA content has been proposed as an optimal an...
Normalisation of real-time RT-PCR reactions
http://www.qpcrupdate.net/hkg.html
Reference Genes / Housekeeping Genes (1). In real-time PCR - page 2. In real-time PCR - page 3. In real-time PCR - page 4. Data normalisation in real-time RT-PCR is a further major step in gene quantification analysis ( Bustin 2002. Any variation in the normalizer will obscure real changes and produce artifactual changes. And cDNA sample loading variation ( Stahlberg 2003. Pfaffl and Hageleit 2001). Or Ribogreen RNA Quantification Kit. Alternatively the rRNA content has been proposed as an optimal an...
Normalisation of real-time RT-PCR reactions
http://www.qpcrupdate.info/hkg.html
Reference Genes / Housekeeping Genes (1). In real-time PCR - page 2. In real-time PCR - page 3. In real-time PCR - page 4. Data normalisation in real-time RT-PCR is a further major step in gene quantification analysis ( Bustin 2002. Any variation in the normalizer will obscure real changes and produce artifactual changes. And cDNA sample loading variation ( Stahlberg 2003. Pfaffl and Hageleit 2001). Or Ribogreen RNA Quantification Kit. Alternatively the rRNA content has been proposed as an optimal an...
Normalisation of real-time RT-PCR reactions
http://www.gmo-qpcr-analysis.com/hkg.html
Reference Genes / Housekeeping Genes (1). In real-time PCR - page 2. In real-time PCR - page 3. In real-time PCR - page 4. Data normalisation in real-time RT-PCR is a further major step in gene quantification analysis ( Bustin 2002. Any variation in the normalizer will obscure real changes and produce artifactual changes. And cDNA sample loading variation ( Stahlberg 2003. Pfaffl and Hageleit 2001). Or Ribogreen RNA Quantification Kit. Alternatively the rRNA content has been proposed as an optimal an...
Normalisation of real-time RT-PCR reactions
http://www.gmo-qpcr-analysis.info/hkg.html
Reference Genes / Housekeeping Genes (1). In real-time PCR - page 2. In real-time PCR - page 3. In real-time PCR - page 4. Data normalisation in real-time RT-PCR is a further major step in gene quantification analysis ( Bustin 2002. Any variation in the normalizer will obscure real changes and produce artifactual changes. And cDNA sample loading variation ( Stahlberg 2003. Pfaffl and Hageleit 2001). Or Ribogreen RNA Quantification Kit. Alternatively the rRNA content has been proposed as an optimal an...
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RefGene - indexes of genes and antibodies | RefGene
Gene of the day. Browse genes by species. Human - Homo sapiens. Mouse - Mus musculus. Rat - Rattus norvegicus. Chimpanzee - Pan troglodytes. Cow - Bos taurus. Dog - Canis lupus familiaris. Zebrafish - Danio rerio. Frog - Xenopus tropicalis. Fly - Drosophila melanogaster. Worm - Caenorhabditis elegans. Chicken - Gallus gallus. Yeast - Saccharomyces cerevisiae. If your interested organisms are not listed above, please use the search box.
RefGenes - condition specific reference genes for RT-qPCR
2 Choose biological context similar to your RT-qPCR experiment. 3 Enter a target gene of interest to see its range of expression. 4 Search for genes highly stable in this biological context and in that range of expression. Cited RefGenes in one of your publications? And get a 6 months free access to GENEVESTIGATOR Professional!
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