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rf-cloning.org

Restriction Free Cloning

A tool for designing restriction free cloning experiments.

http://www.rf-cloning.org/

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Restriction Free Cloning | rf-cloning.org Reviews
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A tool for designing restriction free cloning experiments.
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1 rf-cloning
2 restriction free
3 restriction free cloning
4 restriction-free cloning
5 plasmid
6 overlap extension
7 overlap extension PCR
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9 coupons
10 reviews
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Restriction Free Cloning | rf-cloning.org Reviews

https://rf-cloning.org

A tool for designing restriction free cloning experiments.

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rf-cloning.org rf-cloning.org
1

RF Cloning

http://www.rf-cloning.org/QandA.php

Please note, this site is provided as is, with no warranty. I will only make feature updates that I think are stable, but bugs have been known to lurk in the dark recesses. if you notice something amiss or feel like an important feature should be added, let me know. Q: What is restriction-free cloning? Q: How do I use the website to design an RF cloning project? Q: What is a good starting protocol for my RF cloning project? Between the two target sequences, or the two plasmid sequences, it may be a good ...

2

Draw a Custom Plasmid Map

http://www.rf-cloning.org/savvy.php

Draw Custom Plasmid Map. With great thanks to Dr. Malay Basu for providing the original Savvy. Source code, which was adapted for this website. 1 and 2 cutters.

3

RF-Cloning.org Forum

http://www.rf-cloning.org/fluxbb/index.php

Anything and everything cloning: Go. You are not logged in. Having a hard time getting your reaction to work? Issues with the website. Bug reports, suggestions, compliments :). Are you hacking rf-cloning? Grab a beer, pull up a chair, and chat with the community. Total number of registered users: 290. Total number of topics: 37. Total number of posts: 177. Newest registered user: afahrny. Registered users online: 0. Issues with the website. Atom active topics feed.

4

Restriction Free Cloning SOAP Service

http://www.rf-cloning.org/soap_server.php

If you would like to write your own programs that use the primer design algorithm on this site, the rf-cloning server can handle XML requests using the Simple Object Access Protocol (SOAP). The wsdl file is at http:/ www.rf-cloning.org/classes/rf cloning.wsdl. I have also written a client class in php: http:/ www.rf-cloning.org/classes/rf cloning client.txt. As well as an example Perl client: http:/ www.rf-cloning.org/classes/perl soap client.txt. There are some details on usage in the forum.

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molbiol-tools.ca molbiol-tools.ca

Online Analysis Tools - PCR

http://www.molbiol-tools.ca/PCR.htm

For sites describing PCR theory, as well as companies marketing PCR products you might want to begin by visiting Highveld. For PCR techniques see PCRlink.com. There are several excellent sites for designing PCR primers:. Primer3: WWW primer tool. University of Massachusetts Medical School, U.S.A.) –. GeneFisher - Interactive PCR Primer Design. Universitat Bielefeld, Germany). A very good site allowing great control over primer design. A Untergasser et al. 2007. Nucl. Acids Res. 35. Primer Design and Sear...

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RF Cloning - New USA Journal of Medicine

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Restriction Free Cloning

Popular Plasmids - - - - -. Specify Insert Sites: 1. Or, place 2 exclaimation points (! Directly in the sequence to denote where you want the insert to go. R n]/g,' )". N r]/g,' ); $call numbered triplets plasmid.update('input=' $plasmid sequence,'POST');" /. N r]/g,' )". N r]/g,' ); $call numbered triplets target.update('input=' $input,'POST')" /.

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