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RoseBlite

Generation of Publication Resolution Odyssey Image Files. February 21st, 2013. To save a TIFF file at 600 dpi resolution, open the file in Adobe Photoshop. Select Image Size form the Photoshop Image menu. Uncheck the Resample Image check box. Change the Resolution to 600 pixels/inch. Select Image - Adjustments - Levels - Auto - OK. Select Adjustments - Invert. Label lanes and bands with T tool. Select Image - Image size - 10 inches tall - OK. I like to save at each step (ex: 600dpi, autolevels, labels).

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Generation of Publication Resolution Odyssey Image Files. February 21st, 2013. To save a TIFF file at 600 dpi resolution, open the file in Adobe Photoshop. Select Image Size form the Photoshop Image menu. Uncheck the Resample Image check box. Change the Resolution to 600 pixels/inch. Select Image - Adjustments - Levels - Auto - OK. Select Adjustments - Invert. Label lanes and bands with T tool. Select Image - Image size - 10 inches tall - OK. I like to save at each step (ex: 600dpi, autolevels, labels).

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RoseBlite | roseblite.com Reviews

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Generation of Publication Resolution Odyssey Image Files. February 21st, 2013. To save a TIFF file at 600 dpi resolution, open the file in Adobe Photoshop. Select Image Size form the Photoshop Image menu. Uncheck the Resample Image check box. Change the Resolution to 600 pixels/inch. Select Image - Adjustments - Levels - Auto - OK. Select Adjustments - Invert. Label lanes and bands with T tool. Select Image - Image size - 10 inches tall - OK. I like to save at each step (ex: 600dpi, autolevels, labels).

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roseblite.com

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RoseBlite » Tag » Intracellular

http://roseblite.com/tag/intracellular

Posts Tagged ‘Intracellular’. Intracellular Cytokine Staining – Methanol Permeabilization. December 27, 2012. 1 Activate cells at 37 C. 2 Spin @ 2200RPM for 3 min. 3 Add 4% PFA for 10 min at room temperature (or add directly 16% PFA in medium for 4% PFA final concentration). 4 Wash 1 PBS. 5 Incubate with ice-cold 100% methanol at 4 C for at least 10 min. 6 Wash twice in TBS 1% BSA (USE TBS! And not PBS and no tween. 1.66mL 30% BSA in 50mL 1x TBS.). 7 Incubate with antibody (1/200) 30min in TBS 1% BSA.

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RoseBlite » Tag » western blot

http://roseblite.com/tag/western-blot

Posts Tagged ‘western blot’. Generation of Publication Resolution Odyssey Image Files. February 21, 2013. To save a TIFF file at 600 dpi resolution, open the file in Adobe Photoshop. Select Image Size form the Photoshop Image menu. Uncheck the Resample Image check box. Change the Resolution to 600 pixels/inch. Select Image - Adjustments - Levels - Auto - OK. Select Adjustments - Invert. Label lanes and bands with T tool. Select Image - Image size - 10 inches tall - OK. Posted in Western Blots.

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RoseBlite » Tag » protocol

http://roseblite.com/tag/protocol

Posts Tagged ‘protocol’. Generation of Publication Resolution Odyssey Image Files. February 21, 2013. To save a TIFF file at 600 dpi resolution, open the file in Adobe Photoshop. Select Image Size form the Photoshop Image menu. Uncheck the Resample Image check box. Change the Resolution to 600 pixels/inch. Select Image - Adjustments - Levels - Auto - OK. Select Adjustments - Invert. Label lanes and bands with T tool. Select Image - Image size - 10 inches tall - OK. Posted in Western Blots. 4 Wash 1 PBS.

4

RoseBlite » Tag » ICS

http://roseblite.com/tag/ics

Posts Tagged ‘ICS’. Intracellular Cytokine Staining – Methanol Permeabilization. December 27, 2012. 1 Activate cells at 37 C. 2 Spin @ 2200RPM for 3 min. 3 Add 4% PFA for 10 min at room temperature (or add directly 16% PFA in medium for 4% PFA final concentration). 4 Wash 1 PBS. 5 Incubate with ice-cold 100% methanol at 4 C for at least 10 min. 6 Wash twice in TBS 1% BSA (USE TBS! And not PBS and no tween. 1.66mL 30% BSA in 50mL 1x TBS.). 7 Incubate with antibody (1/200) 30min in TBS 1% BSA.

5

RoseBlite » Tag » staining

http://roseblite.com/tag/staining

Posts Tagged ‘staining’. Intracellular Cytokine Staining – Methanol Permeabilization. December 27, 2012. 1 Activate cells at 37 C. 2 Spin @ 2200RPM for 3 min. 3 Add 4% PFA for 10 min at room temperature (or add directly 16% PFA in medium for 4% PFA final concentration). 4 Wash 1 PBS. 5 Incubate with ice-cold 100% methanol at 4 C for at least 10 min. 6 Wash twice in TBS 1% BSA (USE TBS! And not PBS and no tween. 1.66mL 30% BSA in 50mL 1x TBS.). 7 Incubate with antibody (1/200) 30min in TBS 1% BSA.

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oldblog.maloman.com

Elienai & Christian, married | Maloman Photographers | Maloman Photographers

http://oldblog.maloman.com/2009/08/28/elienai-christian-married

WEDDING AT BALLROOM AT THE BEN, PHILADEPHIA: JACKIE and KYLE. CURRENTLY VIEWING A SINGLE. POST / VIEW ALL POSTS. ELIENAI & CHRISTIAN, MARRIED. Aug 28,2009 / CATEGORY: MARRIED. There are some weddings we shoot where we feel like celebrities and this one was one of those. I can’t even remember how many people came up to me to congratulate me on the killer job we did with the engagement shoot. People behind the scenes:. Ceremony: Plymouth Congregational Church. Grooms Tux: Calvin Klein. You really captured ...

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Generation of Publication Resolution Odyssey Image Files. February 21st, 2013. To save a TIFF file at 600 dpi resolution, open the file in Adobe Photoshop. Select Image Size form the Photoshop Image menu. Uncheck the Resample Image check box. Change the Resolution to 600 pixels/inch. Select Image - Adjustments - Levels - Auto - OK. Select Adjustments - Invert. Label lanes and bands with T tool. Select Image - Image size - 10 inches tall - OK. I like to save at each step (ex: 600dpi, autolevels, labels).

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