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Tuesday, 28 February 2012. Haemocytometer: Determining Cell concentration. 20μl of media containing cells was taken in an eppendorf to which 20μl of trypan blue was added and mixed well. The cover slip was placed on the haemocytometer and 10μl of this solution was loaded to one side of the clean haemocytometer and 10μl more was loaded on to the other side and allowed it to fill by capillary action. The cell number obtained was calculated in a ml. Average of the four squares was calculated. Such forms are...
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