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康润生物 | scientific-chemical.com Reviews

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康润生物,伴您生命科学成功之路

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北京康润诚业生物科技有限公司 GenStar BioSolutions ,是一家专门从事生物技术产品的研发、生产和销售的高新技术企业。 Www gene-star. com.

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GenStar PCR Mix系列明星产品包括普通Taq Mix,高保真Expfu Mix、超保真SuperStar Mix、热启动HotStart Mix等,满足了不同的实验需求,优化的体系,让实验更为轻松、结果更加可靠。

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A501 T4 DNA Ligase. PCR StarMix with Loading Dye" A012 2 Taq. PCR StarMix with Loading Dye. PCR StarMix Loading Dye Free" A015 2 Taq. PCR StarMix Loading Dye Free. PCR StarMix with loading dye" A032 2 HotStart Taq. PCR StarMix with loading dye. PCR StarMix Loading Dye free" A033 2 HotStart Taq. PCR StarMix Loading Dye free. PCR StarMix with Loading Dye" A036 2 HotStart Power Taq. PCR StarMix with Loading Dye. PCR StarMix Loading Dye-free" A037 2 HotStart Power Taq. PCR StarMix Loading Dye-free.

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北京康润诚业生物科技有限公司 GenStar BioSolutions ,是一家专门从事生物技术产品的研发、生产和销售的高新技术企业。 页次 1/2 第一页 上一页 下一页.

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PCR StarMix with Loading Dye" style="line-height:20px;". PCR StarMix with Loading Dye. 1 ml x 100. PCR StarMix Loading Dye Free" style="line-height:20px;". PCR StarMix Loading Dye Free. PCR StarMix Loading Dye free" style="line-height:20px;". A033 2 HotStart Taq. PCR StarMix Loading Dye free. 1 ml x 10. PCR StarMix with Loading Dye" style="line-height:20px;". A036 2 HotStart Power Taq. PCR StarMix with Loading Dye. PCR StarMix Loading Dye-free" style="line-height:20px;". A037 2 HotStart Power Taq.

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GenStar PCR Mix系列明星产品包括普通Taq Mix,高保真Expfu Mix、超保真SuperStar Mix、热启动HotStart Mix等,满足了不同的实验需求,优化的体系,让实验更为轻松、结果更加可靠。

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聚合酶链式反应 Polymerase Chain Reaction,PCR 技术是基因扩增技术的一次重大革新,是分子生物学发展史中的一个重要里程碑。 浓度,从1 mM到3 mM以0.5 mM间隔递增,针对不同模板和引物摸索最佳Mg. 浓度,从1 mM到3 mM以0.5 mM间隔递增,针对不同模板和引物摸索最佳Mg. HotStart PCR TouchDown PCR 巢式PCR. 浓度,从1 mM到3 mM以0.5 mM间隔递增,针对不同模板和引物摸索最佳Mg. 减少酶量,以0.5 U间隔递减 更换质量可靠的酶. HotStart PCR TouchDown PCR 巢式PCR. PCR StarMix with Loading Dye. PCR StarMix Loading Dye Free. A032 2 HotStart Taq. PCR StarMix with loading dye. A033 2 HotStart Taq. PCR StarMix Loading Dye free. A036 2 HotStart Power Taq. A037 2 HotStart Power Taq.

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聚合酶链式反应 Polymerase Chain Reaction,PCR 技术是基因扩增技术的一次重大革新,是分子生物学发展史中的一个重要里程碑。 首先应根据模板的性质 基因组、cDNA、质粒等 和目的片段的大小、GC含量、有无二级结构等,选择合适的DNA聚合酶 参见GenStar PCR产品选择指南。 A 模板用量 以50 μl反应体系为例. 人基因组DNA 0.1 1.0 μg. 大肠杆菌基因组DNA 10 100 ng. Λ DNA 0.5 5 ng. 质粒DNA 0.1 10 ng. 引物长度要满足特异性需要,一般可在18 25个碱基之间 扩增长片段时最好在24 30个碱基之间. G C %含量应尽量控制在40 60%,两条引物的 G C %含量应尽量接近. 20 nt以下 Tm = 2x A T 4x G C. 20 nt以上 Tm = 81.5 0.41x G C% -600/nt nt 引物的碱基数. 01 10 μM,通常可以0.2 μM起始,根据体系不同调整用量.

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