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I am helping in experiments to find genes that regulate growth of E.coli. End aim: improve yield of plasmids from E.coli. And improve growth of E.coli. This blog contains what I've done, learnt, and things which I should read, and some of my ideas. Saturday, March 28, 2015. No sarcasm for at least a year. Pick a project that can last at least the first 5 years of your own lab. Seek out your PI. Fight for the best project. First year is hard, but you'll get through it. If project doesn't work out, kill it.

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I am helping in experiments to find genes that regulate growth of E.coli. End aim: improve yield of plasmids from E.coli. And improve growth of E.coli. This blog contains what I've done, learnt, and things which I should read, and some of my ideas. Saturday, March 28, 2015. No sarcasm for at least a year. Pick a project that can last at least the first 5 years of your own lab. Seek out your PI. Fight for the best project. First year is hard, but you'll get through it. If project doesn't work out, kill it.

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6 explain your logic

7 0 comments

8 treat cells with

9 10nm calyculin a

10 100um sodium orthovanadate

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Logblog | sflogal.blogspot.com Reviews

https://sflogal.blogspot.com

I am helping in experiments to find genes that regulate growth of E.coli. End aim: improve yield of plasmids from E.coli. And improve growth of E.coli. This blog contains what I've done, learnt, and things which I should read, and some of my ideas. Saturday, March 28, 2015. No sarcasm for at least a year. Pick a project that can last at least the first 5 years of your own lab. Seek out your PI. Fight for the best project. First year is hard, but you'll get through it. If project doesn't work out, kill it.

INTERNAL PAGES

sflogal.blogspot.com

1

Logblog: B-Galactosidase staining

http://www.sflogal.blogspot.com/2010/04/b-galactosidase-staining.html

I am helping in experiments to find genes that regulate growth of E.coli. End aim: improve yield of plasmids from E.coli. And improve growth of E.coli. This blog contains what I've done, learnt, and things which I should read, and some of my ideas. Thursday, April 01, 2010. Keep uterus in cold DMEM/F12 until ready to dissect. From dissected embryos in PBS. Fix embryos in Fixation solution shaking on ice for 5min (E7-9) to 15min for adult hearts. Wash with cold PBST 2x. Post fix with 4% PFA 4C O/N.

2

Logblog: A regulatory ribosome

http://www.sflogal.blogspot.com/2011/05/regulatory-ribosome.html

I am helping in experiments to find genes that regulate growth of E.coli. End aim: improve yield of plasmids from E.coli. And improve growth of E.coli. This blog contains what I've done, learnt, and things which I should read, and some of my ideas. Sunday, May 01, 2011. To think that ribosomes might have specialized activities, that there might be different ribosome populations translating different subsets of mRNAs. I'm suddenly reminded at how exciting this is. How revolutionary this thinking is.

3

Logblog: Lab choice advice

http://www.sflogal.blogspot.com/2010/06/lab-choice-advice.html

I am helping in experiments to find genes that regulate growth of E.coli. End aim: improve yield of plasmids from E.coli. And improve growth of E.coli. This blog contains what I've done, learnt, and things which I should read, and some of my ideas. Thursday, June 10, 2010. I want to join a lab where I'm a f*cking moron, so I can learn from everyone". It always help to have a colleague in the same phase of career as you are, so you can bounce ideas off each other". Subscribe to: Post Comments (Atom).

4

Logblog: Whole mount in situ hybridization

http://www.sflogal.blogspot.com/2011/12/whole-mount-in-situ-hybridization.html

I am helping in experiments to find genes that regulate growth of E.coli. End aim: improve yield of plasmids from E.coli. And improve growth of E.coli. This blog contains what I've done, learnt, and things which I should read, and some of my ideas. Thursday, December 01, 2011. Whole mount in situ hybridization. Fix embryos O/N 4% PFA/DEPC PBS. Wash 2x 5min DEPC PBS. Dehydrate through 50% MeOH/ PBS DEPC, then 2 changes of 100% MeOH. Day 1 (DEPC all solutions): Pretreatment and Hybridization. Incubate with...

5

Logblog: B-Gal ELISA

http://www.sflogal.blogspot.com/2010/06/b-gal-elisa.html

I am helping in experiments to find genes that regulate growth of E.coli. End aim: improve yield of plasmids from E.coli. And improve growth of E.coli. This blog contains what I've done, learnt, and things which I should read, and some of my ideas. Friday, June 25, 2010. After trypsin and resuspending in media. Spin and resuspend in 50ul PBS. Used 35ul from the 50ul and add 100ul lysis buffer (dilute from 5x B-gal lysis buffer, Soln 8). Rotate at RT 20min. Spin max speed 15min 4C to remove cell debris.

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susheep.blogspot.com

Expressivo: 2012

http://susheep.blogspot.com/2012/01/2012.html

There's always a way to express. Wednesday, 25 January 2012. In this year, I will. Drink at least 500ml of fluids a day. Know more about ribosomes than my PI. Not sacrifice time with friends and family for work. Subscribe to: Post Comments (Atom). I'm a biologist who likes writing about science. I read the papers so you don't have to. View my complete profile. A life so Siong. Pour tous que je fait s'inquietes. Segeln. ruhe. zwang.

susheep.blogspot.com

Expressivo

http://susheep.blogspot.com/2011/04/i-have-taken-liking-to-bald-trees.html

There's always a way to express. Sunday, 3 April 2011. I have taken a liking to bald trees. Every winter they die and in spring, they're reborned, with a stronger will to live. And even when they lose all their leaves, they still stand tall, majestic and beautiful. Saw this little creature digging out of from the ground to look for food. Cute little fellow. Wiki says it's a vole. It's cherry blossom season =). Subscribe to: Post Comments (Atom). View my complete profile. A life so Siong.

susheep.blogspot.com

Expressivo: Thank you postdocs

http://susheep.blogspot.com/2013/04/thank-you-postdocs.html

There's always a way to express. Wednesday, 24 April 2013. Today I feel good about my project. =) High from lab meeting. Subscribe to: Post Comments (Atom). I'm a biologist who likes writing about science. I read the papers so you don't have to. View my complete profile. A life so Siong. Pour tous que je fait s'inquietes. Segeln. ruhe. zwang.

susheep.blogspot.com

Expressivo: My list from my boss

http://susheep.blogspot.com/2013/12/my-list-from-my-boss.html

There's always a way to express. Friday, 6 December 2013. My list from my boss. Everything is no. 1. Subscribe to: Post Comments (Atom). I'm a biologist who likes writing about science. I read the papers so you don't have to. View my complete profile. My list from my boss. A life so Siong. Pour tous que je fait s'inquietes. Segeln. ruhe. zwang.

susheep.blogspot.com

Expressivo: Fake it until you become it

http://susheep.blogspot.com/2012/11/fake-it-until-you-become-it.html

There's always a way to express. Thursday, 1 November 2012. Fake it until you become. It's rare that I get moved by a video. This video moved me. I'll start this experiment tomorrow. Stretch out, pretend I am powerful and I can do things. King of the lab! My wife and I often publish guest articles or blog posts. For other blog site owners to help increase exposure to our work, as well as. Provide good articles to weblog owners. It really is a win. Also visit my website; check my source. Im using Wordpres...

susheep.blogspot.com

Expressivo: 2013-12

http://susheep.blogspot.com/2013_12_01_archive.html

There's always a way to express. Friday, 6 December 2013. My list from my boss. Everything is no. 1. Subscribe to: Posts (Atom). I'm a biologist who likes writing about science. I read the papers so you don't have to. View my complete profile. My list from my boss. Pour tous que je fait s'inquietes. Segeln. ruhe. zwang. A life so Siong.

susheep.blogspot.com

Expressivo: ELdata

http://susheep.blogspot.com/2011/11/eldata.html

There's always a way to express. Monday, 28 November 2011. Got a score/ data request from someone in an electone forum. felt like it's something from a previous life. Don't even remember where I got the data from, how meticulous I was in compressing the data, uploading them, unprotecting things, archiving others. Those were the days. Subscribe to: Post Comments (Atom). I'm a biologist who likes writing about science. I read the papers so you don't have to. View my complete profile. A life so Siong.

susheep.blogspot.com

Expressivo: Innocent

http://susheep.blogspot.com/2012/05/innocent.html

There's always a way to express. Wednesday, 2 May 2012. I've always liked this song, since we first played in lower Sec. My attempt at creating tracks and mixes. It's not perfect, but it's been a while since I recorded anything and I really wanted to record this. This is one of the things that's keeping me sane these days. Subscribe to: Post Comments (Atom). I'm a biologist who likes writing about science. I read the papers so you don't have to. View my complete profile. A life so Siong.

susheep.blogspot.com

Expressivo: Intimidating

http://susheep.blogspot.com/2013/06/intimidating.html

There's always a way to express. Monday, 17 June 2013. First time in my life, I've been described as intimidating. What has happened in the last half year to earn me this status? Jammed today, first time in 3 years? It was awesome. Didn't know we could still play. and we sounded decent! We played through most of the songs that we wanted to. And still remembered how to play some old stuff that we used to know. I want to do this again. Subscribe to: Post Comments (Atom). View my complete profile.

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Logblog

I am helping in experiments to find genes that regulate growth of E.coli. End aim: improve yield of plasmids from E.coli. And improve growth of E.coli. This blog contains what I've done, learnt, and things which I should read, and some of my ideas. Saturday, March 28, 2015. No sarcasm for at least a year. Pick a project that can last at least the first 5 years of your own lab. Seek out your PI. Fight for the best project. First year is hard, but you'll get through it. If project doesn't work out, kill it.

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8203;The San Francisco Log Cabin Republicans is the founding chapter of the nation's largest Republican LGBT organization. Since 1977, the San Francisco Log Cabin Republicans have promoted the fight for equality through outreach, political campaigns, and education. 8203;Please join us. At our next meeting. To learn more about our group! Join Log Cabin Republicans of San Francisco for only $50! New and renewing members get the following:. Log Cabin Republicans of San Francisco pen. 8203;Click on Join Us.

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Saint Faustin Log House - Maison en Bois Rond

Saint Faustin Log House - Maison en Bois Rond. ST-FAUSTIN - Quality log house on Royal Laurentian. View my complete profile. Wednesday, July 2, 2008. Click them to enlarge image. Maison de Qualité en bois rond sur le Golf Royal Laurentien. Ski, 5 min de Mont Blanc, 15 min de Mont Tremblant. Construit en 2006 sur terrain boise de 56,000 pi.car. 3 chambres a couché, 2 1/2 salle de bain. Concept ouvert au premier étage. Aspirateur central. Syst. Cliquez les photos pour aggrandir. Subscribe to: Posts (Atom).

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