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SILAC - Stable isotope labeling by amino acids in cell culture

SILAC is an approach for in vivo incorporation of a label into proteins for mass spectrometry (MS)-based quantitative proteomics

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SILAC - Stable isotope labeling by amino acids in cell culture | silac.org Reviews
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SILAC is an approach for in vivo incorporation of a label into proteins for mass spectrometry (MS)-based quantitative proteomics
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SILAC - Stable isotope labeling by amino acids in cell culture | silac.org Reviews

https://silac.org

SILAC is an approach for in vivo incorporation of a label into proteins for mass spectrometry (MS)-based quantitative proteomics

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SILAC :: Useful Links

http://www.silac.org/links

Supported in part by an NIH Roadmap grant "Technology Center for Networks and Pathways of Lysine Modification". Useful Links for SILAC. Http:/ www.isotope.com. Http:/ www.pil.sdu.dk. Http:/ www.invitrogen.com. Http:/ www.piercenet.com. Http:/ www.dundeecellproducts.com. Http:/ msquant.sourceforge.net. Http:/ www.ionsource.com. Http:/ www.hprd.org. Http:/ www.athenaes.com. This site is developed and maintained by PandeyLab.

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SILAC :: Recipes

http://www.silac.org/recipes

Supported in part by an NIH Roadmap grant "Technology Center for Networks and Pathways of Lysine Modification". Dulbecco's Eagle's minimum essential medium deficient in amino acids arginine and lysine was custom synthesized by AthenaES (Baltimore, MD). Add amino acids L-lysine (0.798 mM) and. Stable isotopic amino acids used in SILAC media. L-Lysine 2HCl (223.13). 4,4,5,5-D4). L-Lysine HCl (186.67). 4,4,5,5-D4). L-Lysine 2HCl (225.1). L-Lysine HCl (188.6). L-Lysine 2HCl (227.1). L-Lysine HCl (190.59).

3

SILAC :: Reagents

http://www.silac.org/reagents

Supported in part by an NIH Roadmap grant "Technology Center for Networks and Pathways of Lysine Modification". Cambridge Isotope laboratories Inc, Andover MA.). L-Lysine is a stable isotope of. L-Lysine and is 6 Da heavier than. Catalog # CLM-2265 (Cambridge Isotope laboratories Inc, Andover MA.). L- Arginine is a stable isotope of. L- Arginine and is 6 Da heavier than. Catalog # CNLM-539 (Cambridge Isotope laboratories Inc, Andover MA.). L- Arginine is a stable isotope of. N) (Cat No#33,215-1, Sigma-Al...

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SILAC :: Stable isotope labeling with amino acids in cell culture

http://www.silac.org/index_html

Supported in part by an NIH Roadmap grant "Technology Center for Networks and Pathways of Lysine Modification". This site is developed and maintained by PandeyLab.

5

SILAC :: Applications

http://www.silac.org/applications

Supported in part by an NIH Roadmap grant "Technology Center for Networks and Pathways of Lysine Modification". Differential expression of proteins and identification of disease biomarkers. Gronborg et al.,. Similar approach has been undertaken by An et al.,. To identify differentially expressed proteins by human retinal pigment epithelium cell cultures derived from donors with age related macular degeneration and RPE cells from age matched healthy donors. Following stimulation by an extracellular ligand...

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Jump to search field. SV Core Facilities and Technology Platforms. Proteomics Core Facility and Technology Platform. Proteomics Core Facility and Technology Platform. Job and Master Projects opening. For the lab only. After a LC-MS analysis, a huge quantity of data must be stored and analysed. Different bioinformatics tools. The general pipeline of the facility consists of:. Proteome Discoverer 1.4. The Global Proteome Machine Organization. An analysis with Omssa can be done upon request. Protein identif...

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NHLBI Proteomics Centers

http://www.nhlbi-proteomics.org/pages/tools

Last updated on: August 25, 2015. Omics Integration in Biology and Medicine Workshop (Day 1). Tuesday, June 19, 2012. Omics Integration in Biology and Medicine Workshop (Day 2). Wednesday, June 20, 2012. Census - Software Tool for Quantitative Analysis. High-throughput mass spectrometry data from shotgun proteomics experiments necessitate an efficient and automated way to analyze large amounts of quantitative data. Software tool for researching annotations of proteins: open-source protein annotation soft...

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The Bioblogist: January 2015

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Science from the bench, press and media, plus a little bit of silliness. Monday, 26 January 2015. Decoding Annie Parker Review - "BRCA1ng bad". Decoding Annie Parker is a film about the discovery of the BRCA1 gene and its role in breast cancer. I can't really recommend the film as I'm not that interested in such dramas but I guess it does do a good job of highlighting how long research can take and how the knowledge it obtains can provide comfort even when it doesn't immediately provide a solution. I...

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Pandey Lab Links

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Human Proteome Map - A draft map of the human proteome. Human Proteinpedia - A community portal for sharing and integration of human protein data. Stable isotope labeling with amino acids in cell culture (SILAC). Human Protein Reference Database. NetPath - A curated resource of signal transduction pathways. Human Protein Atlas (HPA). Databases: nrdb95, swissprot, pdb, sp nrdb, nrdb.

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NHLBI Proteomics Centers

http://nhlbi.ctrl.ucla.edu/pages/tools

Last updated on: August 25, 2015. Omics Integration in Biology and Medicine Workshop (Day 1). Tuesday, June 19, 2012. Omics Integration in Biology and Medicine Workshop (Day 2). Wednesday, June 20, 2012. Census - Software Tool for Quantitative Analysis. High-throughput mass spectrometry data from shotgun proteomics experiments necessitate an efficient and automated way to analyze large amounts of quantitative data. Software tool for researching annotations of proteins: open-source protein annotation soft...

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Athena Technical Support

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Items in my cart [0]. Simple Solutions for Complex Proteins. Genie&#174 Family of Products. Order Your Free Catalog. Data Sheets and Manuals. Technical Briefs / White Papers. Plasmid Maps and Sequences. Request a Certification of Analysis. Request a Product Formulation. To speak with one of our Staff Scientists, call 1-888-892-8408 or click the link below to fill out an electronic form. Safe Guidelines for Product Use. Custom Cell Culture Media. How do I order? How do I set up an account?

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Wonder!: SILAC :: Stable isotope labeling with amino acids in cell culture

http://biowonder.blogspot.com/2007/01/silac-stable-isotope-labeling-with.html

Biology, medicine, network, complex system, anything that can be called Wonderful! SILAC : Stable isotope labeling with amino acids in cell culture. SILAC : Stable isotope labeling with amino acids in cell culture. N) Thus in an experiment, two cell populations are grown in culture media that are identical except that one of them contains a ‘light’ and the other a ‘heavy’ form of a particular amino acid (e.g. The site is developed and maintained by Pandey Lab. Posted by Nyu @ 1/12/2007 05:02:00 PM.

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SILAC - Stable isotope labeling by amino acids in cell culture

Supported in part by an NIH Roadmap grant "Technology Center for Networks and Pathways of Lysine Modification". This site is developed and maintained by PandeyLab.

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