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CD133 cells budding from the adherent cell surface by asymetric cell division. Cells were stained with CD133/1 (AC133)-PE. Stem Cell Extraction References. Any comments or improvements with regard to adult stem cell collection, isolation, culturing or preservation procedures is greatly appreciated and may be forewarded to: e-m@mail.com, Thank you. December 18, 2004. Non Flash hyperlink controls are duplicated for browsers as Mozilla Firefox. Updated: Monday, December 27, 2004 0:39 AM.

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CD133 cells budding from the adherent cell surface by asymetric cell division. Cells were stained with CD133/1 (AC133)-PE. Stem Cell Extraction References. Any comments or improvements with regard to adult stem cell collection, isolation, culturing or preservation procedures is greatly appreciated and may be forewarded to: e-m@mail.com, Thank you. December 18, 2004. Non Flash hyperlink controls are duplicated for browsers as Mozilla Firefox. Updated: Monday, December 27, 2004 0:39 AM.

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2 site map

3 laboratory equipment

4 preservation procedures

5 adipose retrieval references

6 homepage

7 equipment

8 procedures

9 retrieval

10 extraction

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Home Page | stemcelllab.tripod.com Reviews

https://stemcelllab.tripod.com

CD133 cells budding from the adherent cell surface by asymetric cell division. Cells were stained with CD133/1 (AC133)-PE. Stem Cell Extraction References. Any comments or improvements with regard to adult stem cell collection, isolation, culturing or preservation procedures is greatly appreciated and may be forewarded to: e-m@mail.com, Thank you. December 18, 2004. Non Flash hyperlink controls are duplicated for browsers as Mozilla Firefox. Updated: Monday, December 27, 2004 0:39 AM.

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stemcelllab.tripod.com

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Extraction Adipose

http://stemcelllab.tripod.com/extraction.htm

Adipose Stem Cell Extraction References:. The American Society for Cell Biology. Mol Biol Cell. 2002 December; 13(12): 4279–4295. Doi: 10.1091/mbc.E02-02-0105. Human Adipose Tissue Is a Source of Multipotent Stem Cells. Patricia A. Zuk,. Daniel A. De Ugarte,. Jerry I. Huang,. Zeni C. Alfonso,. John K. Fraser,. And Marc H. Hedrick. Departments of Surgery and Orthopedics, Regenerative Bioengineering and Repair Laboratory, UCLA School of Medicine, Los Angeles, California 90095; and. The antibodies and comme...

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Home Page

http://stemcelllab.tripod.com/index.htm

CD133 cells budding from the adherent cell surface by asymetric cell division. Cells were stained with CD133/1 (AC133)-PE. Stem Cell Extraction References. Any comments or improvements with regard to adult stem cell collection, isolation, culturing or preservation procedures is greatly appreciated and may be forewarded to: e-m@mail.com, Thank you. December 18, 2004. Non Flash hyperlink controls are duplicated for browsers as Mozilla Firefox. Updated: Monday, December 27, 2004 0:39 AM.

3

Adipose Retrieval

http://stemcelllab.tripod.com/retrieval.htm

Http:/ www.tulipmedical.com. The Tumescent Technique is simply a method of infusing (infiltrating) a large volume of solution into the subcutaneous space. The technique provides for satisfactory anesthesia to perform subcutaneous soft tissue removal, and adequate vasoconstriction to lessen blood loss associated with this procedure. The following are examples of formulas for the tumescent solution:. 1 cc epinephrine 1:1000. 1 OCC 3% sodium bicarbonate. 50cc marcaine .5%. 1,000 ml normal saline. FournierPF...

4

Procedures

http://stemcelllab.tripod.com/procedure.htm

Adipose and PBSC Preservation Procedures. Adipose Derived Stem Cell (ADSC) Retrieval procedure:. Wash aspirates, with sterile phosphate-buffered saline (PBS). Treat aspirates with 0.075% collagenase in PBS for 30 min at 37 C with gentle agitation; inactivate collagenase with an equal volume of DMEM/10% fetal bovine serum (FBS) and the infranatant centrifuged for 10 min at low speed. Culture processed lipoaspirate in 72 h before analysis. Isolate based on: CD13, CD44, CD49d, CD106, STRO-1 (?

5

Lab Equipment

http://stemcelllab.tripod.com/labEquip.htm

Lab Equipment for Adipose and Peripheral Blood Stem Cell. Collection, Culturing and Preservation. Tulip CellFriendly Syringe Instrumentation. Standard Tissue Harvesting Cannula SuperLuerLok Adapters, $275. Http:/ www.tulipmedical.com/canulas.html 800-325-6526. 60cc Syringe Toomey Tip (steril). 60cc - 35cc - 10cc - Luer - SuperLuer Lok. 14mm (injector only) - 1.6 mm (injector only) - 2.1mm - 2.4mm - 2.7mm - 3.0mm - 3.7mm - 4.6 mm. 8 cm (injector only) - 15cm - 25cm - 35cm. 8226; Ultrasonic aspirators:.

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CD133 cells budding from the adherent cell surface by asymetric cell division. Cells were stained with CD133/1 (AC133)-PE. Stem Cell Extraction References. Any comments or improvements with regard to adult stem cell collection, isolation, culturing or preservation procedures is greatly appreciated and may be forewarded to: e-m@mail.com, Thank you. December 18, 2004. Non Flash hyperlink controls are duplicated for browsers as Mozilla Firefox. Updated: Monday, December 27, 2004 0:39 AM.

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