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DGGE Help: July 2006
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Thursday, July 06, 2006. Protocol for pouring Ingeny DGGE Gels. IMMEDIATE LINK TO DGGE GUIDE. I am posting a protocol for pouring Ingeny DGGE gels prepared by Cameron Blackford, a researcher working in my laboratory. It can be downloaded in PDF. Posted by Stefan J. Green @ 11:38 AM. DGGE Book Chapter - Green, Leigh and Neufeld. DGGE Guide in Word Format. DGGE Guide in PDF Format. Ingeny Gel Pouring Document in PDF Format. New manuscript about reproducibility of GC clamps. SB buffer for DGGE?
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DGGE Help: April 2007
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Monday, April 16, 2007. Linked in Spanish Wikipedia. IMMEDIATE LINK TO GUIDE -. Thanks for all your support and wonderful comments regarding this website. It has been wonderful interacting with researchers from around the world. I love hearing from people who have found this website useful and those who need some help. So, please continue writing. On a side note, I'm pleased to say that this website is referenced in the spanish Wikipedia entry. Posted by Stefan J. Green @ 9:27 AM. DGGE Guide in PDF Format.
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DGGE Help: January 2006
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Tuesday, January 17, 2006. First DGGE Update of 2006. IMMEDIATE LINK TO DGGE GUIDE. Neufeld, Josh D., Mohn, William W. and de Lorenzo, Victor (2006) Composition of microbial communities in hexachlorocyclohexane (HCH) contaminated soils from Spain revealed with a habitat-specific microarray. Environmental Microbiology 8, 126-140. Other topics of interest:. An "In Press" publication showing DGGE analysis of dsrB. Geets, J. et al. In press. dsrB. Maurizio Castaldini (CRA - ISSDS, Italy) refers us to "Gel Ha...
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DGGE Help: March 2007
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Tuesday, March 13, 2007. DGGE Review in Soil Science Journal. A new review of DGGE is available in the Soil Science Society of America Journal. Details are listed below. Nakatsu, C. H. Soil Microbial Community Analysis Using Denaturing Gradient Gel Electrophoresis. Soil Sci Soc Am J 2007 71: 562-571. Posted by Stefan J. Green @ 8:47 AM. DGGE Book Chapter - Green, Leigh and Neufeld. DGGE Guide in Word Format. DGGE Guide in PDF Format. Ingeny Gel Pouring Document in PDF Format. SB buffer for DGGE?
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DGGE Help: August 2007
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Friday, August 03, 2007. New Article in Print. IMMEDIATE LINK TO GUIDE -. Just a quick note to update a previous post. My recent article -. Green, S.J., Michel Jr., F.C., Hadar, Y. and D. Minz. [2007]. Contrasting patterns of seed and root colonization by bacteria from the genus Chryseobacterium. And from the family Oxalobacteraceae. ISME Journal. 1,. Is now in print. Cheers, Stefan. Posted by Stefan J. Green @ 9:51 AM. DGGE Book Chapter - Green, Leigh and Neufeld. DGGE Guide in Word Format.
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DGGE Help: February 2006
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Friday, February 17, 2006. IMMEDIATE LINK TO DGGE GUIDE. Just a brief update here. Dr. Marcell Nikolausz from the Department of Bioremediation at the UFZ Centre for Environmental Research in Leipzig, Germany wrote in to with two references regarding recovery of DGGE sequences. Xueli Zhang, Xing Yan, Pingping Gao, Linghua Wang, Zhihua Zhou and Liping Zhao. 2004. Nikolausz M, Sipos R, Revesz S, Szekely A, Marialigeti K. Posted by Stefan J. Green @ 9:10 AM. DGGE Book Chapter - Green, Leigh and Neufeld.
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DGGE Help: December 2006
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Wednesday, December 20, 2006. Some more publications of interest and silver staining. Immediate link to DGGE Guide -. Please notify me if there are links that are no longer valid. - Thanks, Stefan. Some newer publications of potential interest to the DGGE community:. A double-gradient (acrylamide and denaturant):. Analysis of Bacterial Communities in Seagrass Bed Sediments by Double-Gradient Denaturing Gradient Gel Electrophoresis of PCR-Amplified 16S rRNA Genes. Volume 52, Number 4 / November, 2006.
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DGGE Help: January 2008
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Sunday, January 13, 2008. Alternative Electrophoresis Buffer to Replace TAE or TBE. IMMEDIATE LINK TO GUIDE -. I am revising this submission to be a little clearer. I would like to advocate the use of three new electrophoresis buffers. Links to the relevant manuscripts are below. Also found below is a link to the company Faster-better-media selling these buffers. 2) LA Buffer 5 mM lithium acetate. 3) LB Buffer 1 mM lithium boric acid (lithium hydroxide pH buffered to pH 8.5 with boric acid). The authors ...
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DGGE Help: June 2006
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Sunday, June 04, 2006. DGGE for Phylum Bacteroidetes. IMMEDIATE LINK TO DGGE GUIDE. Not to toot my own horn, but if you are interested, please check out my recent manuscript in Applied and Environmental Microbiology. We describe a nice nested PCR approach for analyzing general bacteria in conjunction with bacteria from the phylum Bacteroidetes. - Stefan. Posted by Stefan J. Green @ 11:19 AM. DGGE Book Chapter - Green, Leigh and Neufeld. DGGE Guide in Word Format. DGGE Guide in PDF Format.
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