tcf.epfl.ch

Transgenic Core Facilities | EPFL

Jump to search field. School of Life Sciences. More SV core facilities. How to find us. Pronuclear Injection of DNA. Pronuclear Injection of DNA. LV Production / Titration. LV Production / Titration. Our mission is to provide a centralized resource. And state-of-the-art technology for the generation of transgenic animals. We can perform direct. Pronuclear injection of DNA. Which has been the standard method of. For more than three decades. As an attractive alternative, we are one of the very few platforms.

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Transgenic Core Facilities | EPFL | tcf.epfl.ch Reviews

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Jump to search field. School of Life Sciences. More SV core facilities. How to find us. Pronuclear Injection of DNA. Pronuclear Injection of DNA. LV Production / Titration. LV Production / Titration. Our mission is to provide a centralized resource. And state-of-the-art technology for the generation of transgenic animals. We can perform direct. Pronuclear injection of DNA. Which has been the standard method of. For more than three decades. As an attractive alternative, we are one of the very few platforms.

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Transgenic Core Facilities | EPFL | tcf.epfl.ch Reviews

https://tcf.epfl.ch

Jump to search field. School of Life Sciences. More SV core facilities. How to find us. Pronuclear Injection of DNA. Pronuclear Injection of DNA. LV Production / Titration. LV Production / Titration. Our mission is to provide a centralized resource. And state-of-the-art technology for the generation of transgenic animals. We can perform direct. Pronuclear injection of DNA. Which has been the standard method of. For more than three decades. As an attractive alternative, we are one of the very few platforms.

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tcf.epfl.ch

1

Time line | TCF

http://tcf.epfl.ch/page-75815-en.html

Jump to search field. School of Life Sciences. More SV core facilities. Pronuclear Injection of DNA. How to find us. Pronuclear Injection of DNA. Pronuclear Injection of DNA. LV Production / Titration. LV Production / Titration. Per project we inject 300 oocytes that must be sufficient to obtain 2 transgenics animals (based on our internal statistics). On average 3 injection sessions are required to reach this number of oocytes. This can be realized in around 3 weeks. 1 session of injection.

2

About us | TCF

http://tcf.epfl.ch/page-75906-en.html

Jump to search field. School of Life Sciences. More SV core facilities. How to find us. Pronuclear Injection of DNA. Pronuclear Injection of DNA. LV Production / Titration. LV Production / Titration. How to find us. The platform is under the supervision of. The platform is part of the. Center of PhenoGenomics (FSV – EPFL). Isabelle Barde is in charge of the platform. Isabelle, Sonia and Sandra work 50% on the platform and 50% in the Didier Trono’s lab. Top of the page. Updated 08.09.2014.

3

Our outcomes | TCF

http://tcf.epfl.ch/page-6768-en.html

Jump to search field. School of Life Sciences. More SV core facilities. How to find us. Pronuclear Injection of DNA. Pronuclear Injection of DNA. LV Production / Titration. LV Production / Titration. Genetic features of LV-mediated transenic animals. Integration occurs early (2- to 4-cell stage). Integration results in proviral mosaicism. Proviral copy number in the animal can exceed 15-20. Integration favors transcribed regions (. Sauvain MO et al. J Virol 2008. Vector-dependent (from 100% to 32%).

4

Procedure | TCF

http://tcf.epfl.ch/page-75820-en.html

Jump to search field. School of Life Sciences. More SV core facilities. Pronuclear Injection of DNA. How to find us. Pronuclear Injection of DNA. Pronuclear Injection of DNA. LV Production / Titration. LV Production / Titration. The investigator has to contact the facility:. And fill in the. Importantly the genotyping protocol to identify transgenic animals has to set up by the investigator before delivering DNA to the facility. The investigator has to send the plasmid sequence in genbank format. This nu...

5

Pronuclear Injection of DNA | TCF

http://tcf.epfl.ch/page-56799-en.html

Jump to search field. School of Life Sciences. More SV core facilities. Pronuclear Injection of DNA. How to find us. Pronuclear Injection of DNA. Pronuclear Injection of DNA. LV Production / Titration. LV Production / Titration. Pronuclear Injection of DNA. Pronuclear Injection of DNA. Pronuclear Injection of DNA. Two types of DNA are considered:. And large, genomic DNA-based transgenes :. It consists of a promoter, a complete protein-coding sequence from a. Signal cloned into a high-copy-number plasmid.

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limna.ch

Platforms & Core Facilities | Limna - Lausanne Integrative Metabolism Nutrition Alliance

http://www.limna.ch/platforms_and_core_facilities

PLATFORMS and CORE FACILITIES. UNIL CHUV - EPFL. Rue du Bugnon 7. Tel 41 21 692 55 53. Fax 41 21 692 55 95. Platforms and Core Facilities. Design by Parenthèse NOW.

bioengineering.epfl.ch

Core Facilities | IBI

http://bioengineering.epfl.ch/cores

Interfaculty Institute of Bioengineering. Interfaculty Institute of Bioengineering. Systems and Computational Biology. Systems Physiology and Immunoengineering. Molecular, Cell and Tissue Engineering. PhD Program in Biotech and BioE (EDBB). Monthly IBI-EDBB Grad Student Mini-Symposia. Prize awarded by the President of the Confederation. 3D-nanoprinting to turbocharge microscopes. A new path for killing pathogenic bacteria. A membrane that generates electricity from seawater and fresh water. Using gold an...

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tcf.epfl.ch

Transgenic Core Facilities | EPFL

Jump to search field. School of Life Sciences. More SV core facilities. How to find us. Pronuclear Injection of DNA. Pronuclear Injection of DNA. LV Production / Titration. LV Production / Titration. Our mission is to provide a centralized resource. And state-of-the-art technology for the generation of transgenic animals. We can perform direct. Pronuclear injection of DNA. Which has been the standard method of. For more than three decades. As an attractive alternative, we are one of the very few platforms.

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