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Teaching platform for biochemistry, biophysical chemistry, and molecular biology using the SSB protein as a model system

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Teach with SSB - Home | teachwithssb.com Reviews
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Teaching platform for biochemistry, biophysical chemistry, and molecular biology using the SSB protein as a model system
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Teach with SSB - Home | teachwithssb.com Reviews

https://teachwithssb.com

Teaching platform for biochemistry, biophysical chemistry, and molecular biology using the SSB protein as a model system

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1

Reading List - Teach with SSB

http://www.teachwithssb.com/reading-list.html

Suggested list of papers on SSB proteins. As an organizer/mobilizer of genome maintenance complexes. Shereda RD, Kozlov AG, Lohman. TM, Cox MM, Keck JL. Crit Rev Biochem Mol Biol. 2008 Sep-Oct;43(5):289-318. doi: 10.1080/10409230802341296 . Review. Large-scale overproduction and rapid purification of the Escherichia coli ssb. Gene product. Expression of the ssb. Gene under lambda PL control. TM, Green JM, Beyer RS. Biochemistry. 1986 Jan 14;25(1):21-5. TM, Overman LB. Raghunathan S, Kozlov AG, Lohman.

2

Experiments - Teach with SSB

http://www.teachwithssb.com/experiments.html

Course Material for Individual Experiments. Handouts for each experiment from the various semesters can be downloaded from the list below. If you generate different experiments and would like us to add them to this list please send it to us. We can review them, speak with you and upload it for everybody to adopt. Thank You.​. Syllabus (BIOL 4102) - Experimental Molecular Biology, Fall 2016. Marqutte University. Syllabus mu 2016 fall.pdf. Polymerase Chain Reaction to amplify SSB gene of interest. Experime...

3

Teach with SSB - Blog

http://www.teachwithssb.com/blog.html

Stop Flow FRET analysis of DNA wrapping around SSB. Stop Flow Experiments - SSB:DNA Binding Kinetics. Experiments with increasing signals are SSB proteins binding to fluorescently labeled DNA substrate. Experiments with decreasing signal are intrinsic trp changes in the SSB protein upon binding to DNA. Crystals - - yay! Students setup crystallization trays with SSB-DNA complexes. PhewSSB proteins were purified this week. Science and Art with Artist Jenson Gendron. Week 2. SSB cloning into pCDF Vector.

4

BIOL4102 Data - Teach with SSB

http://www.teachwithssb.com/biol4102-data.html

Week 1. PCR Lab Data. Data for pcr lab week 1 biol 4102.pptx. Week 2. Restriction Digest Data for Cloning. Week 2 cloning digest data.pptx. Week 3. Cloning. Tuesday group gel extraction figures.pptx. Thursday group gel extraction figures.pptx. Week 4. Analysis of Clones. Tuesday group clone screening figures.pptx. Thursday group clone screening figures.pptx. Week 5-6. Protein Expression and SSB Purification. Ssb protein purification gels.pptx. Week 8. EMSA Data. Tue group emsa data.ppt. Week 10: FRET Data.

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EDUCATION & OUTREACH - The Antony Lab

http://www.antonylab.org/education--outreach.html

M-STEAM Outreach Program is Underway. Teach With SSB Program is Underway. Our laboratory teaching module with the SSB protein as a model system is finally in place. The course material and information can be found at the following website:. 8203;Please feel to contact us if you would like to adopt our SSB based experiments, or the entire set of experiments from this years module, for your Biochemistry, Molecular Biology or Biophysical Chemistry Lab course. High School Students - Summer Research.

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NEWS - The Antony Lab

http://www.antonylab.org/news.html

Parker's Paper is Accepted for Publication. Parker's work on using the SSB protein from. As a tool to monitor Rad51 disassembly by Srs2 has been accepted for publication in PLOS One. Congrats! Nilisha Receives Two Travel Awards. New High School Student. Pratyusha Emkay, a junior high school student from Greenfield High School has joined the lab. Welcome! Ang ZY, Ledbetter R, Shaw S, Pence NK, Tokmina-Lukaszewska M, Eilers BJ, Guo Q, Pokhrel N, Cash VL, Dean DR,. Bothner B, Peters JW, Seefeldt LC. Our inv...

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Teach with SSB - Home

Overexpression strains. We would be delighted to hear from you if you have ideas to improve the experiments or to add new experiments to our library. Thanks for your interest! Advantages of using the SSB protein for teaching. 8203; Rapid, Column-Free Purification. 8203; Long-term Stability. These images were generated by the students in the BIOL4102 class (Fall 2015). They have conveyed their scientific findings using a cohesive artistic rendering, suitable for public dissemination. A SDS-PAGE gel showin...

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