tol2kit.genetics.utah.edu

Main Page - Tol2kit

Tol2kit v1.2, released November 2007. Generating stable transgenic zebrafish lines has historically been laborious, for three reasons:. Building transgenesis constructs by conventional subcloning can be difficult, especially when using long coding or regulatory sequences. Injecting plasmid DNA yields mosaic transient expression and low germline transgenesis rates. The Tol2kit uses site-specific recombination-based cloning ( multisite Gateway technology. It includes a variety of widely-useful entry clones.

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Tol2kit v1.2, released November 2007. Generating stable transgenic zebrafish lines has historically been laborious, for three reasons:. Building transgenesis constructs by conventional subcloning can be difficult, especially when using long coding or regulatory sequences. Injecting plasmid DNA yields mosaic transient expression and low germline transgenesis rates. The Tol2kit uses site-specific recombination-based cloning ( multisite Gateway technology. It includes a variety of widely-useful entry clones.

<META> KEYWORDS

1 Main Page

2 Att site sequences

3 Basic Gateway principles

4 Inserts and ends

5 Invitrogen manual

6 List of entry and destination vectors

7 Protocols

8 Sample results with the Tol2kit

9 Sources

10

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main page,from tol2kit,jump to navigation,contents,the tol2kit,about this site,clone distribution,citing the tol2kit,links,revision history,wiki help,edit,including hsp70,and beta actin,transgenesis marker,basic gateway principles,protocols,and a preprint

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Main Page - Tol2kit | tol2kit.genetics.utah.edu Reviews

https://tol2kit.genetics.utah.edu

Tol2kit v1.2, released November 2007. Generating stable transgenic zebrafish lines has historically been laborious, for three reasons:. Building transgenesis constructs by conventional subcloning can be difficult, especially when using long coding or regulatory sequences. Injecting plasmid DNA yields mosaic transient expression and low germline transgenesis rates. The Tol2kit uses site-specific recombination-based cloning ( multisite Gateway technology. It includes a variety of widely-useful entry clones.

INTERNAL PAGES

tol2kit.genetics.utah.edu

1

Invitrogen manual - Tol2kit

http://tol2kit.genetics.utah.edu/index.php/Invitrogen_manual

Here is an archived copy of the Invitrogen 3-insert multisite Gateway manual, version D, 3-07-07. We originally had a live link to the Invitrogen site, but after this link broke it seemed easier to hard-archive a copy. Invitrogen manual version D. Presumably att sites from different generations of the kits are still functionally compatible. Retrieved from " http:/ tol2kit.genetics.utah.edu/index.php/Invitrogen manual. Log in / create account. This page was last modified 12:28, 12 October 2007.

2

Main Page - Tol2kit

http://tol2kit.genetics.utah.edu/index.php/Special:Whatlinkshere/Main_Page

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Tol2Kit:About - Tol2kit

http://tol2kit.genetics.utah.edu/index.php/Tol2Kit:About

A multisite Gateway-based construction kit for building zebrafish expression vectors in a Tol2 transposon backbone. The constructs in the initial release of the Tol2kit (Jan 2007) were built primarily by Kristen Kwan and Esther Fujimoto in the Chien lab, with contributions from Chi-Bin Chien, Ben Mangum, Melissa Hardy, and Doug Campbell. Retrieved from " http:/ tol2kit.genetics.utah.edu/index.php/Tol2Kit:About. Log in / create account. This page was last modified 00:35, 30 January 2007.

5

Recent changes - Tol2kit

http://tol2kit.genetics.utah.edu/index.php/Special:Recentchanges

Track the most recent changes to the wiki on this page. Below are the last 50. Changes in the last 7. Days, as of 11:36, 13 March 2017. Changes in last 1. Show new changes starting from 11:36, 13 March 2017. Retrieved from " http:/ tol2kit.genetics.utah.edu/index.php/Special:Recentchanges. Log in / create account.

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zdmsociety.org

Resources | ZDM Society

http://zdmsociety.org/resources

Mail : info@zdmsociety.org. News & Resources. Zebrafish and Human Disease. Cancer Research Interest Group. Education Research Interest Group. GI Metabolism Research Interest Group. Hematology Research Interest Group. Infection & Inflammation Research Interest Group. Cardiac & Skeletal Muscle Research Interest Group. Neural Research Interest Group. Skeletal Research Interest Group. Toxicology Research Interest Group. Zebrafish and Human Disease. Resources for Zebrafish Researchers. Design tool is a web to...

wythelab.com

Etc — Wythe Lab

http://www.wythelab.com/etc

Some links will open in a new window). Baylor College of Medicine. BCM Graduate Program in Molecular Physiology & Biophysics. BCM Graduate Program in Developmental Biology. BCM Graduate Program in Molecular Physiology. Sue McConnell, PhD on Effective PowerPoint Presentations. Jonathan Shewchuk on PowerPoint presentations. Great Advice on Journal Club Presentations (C. Thummel). Knockout Mouse Project (KOMP). International Mouse Phenotyping Consortium (IMPC). Gene Expression Atlas (EMAP).

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tol2 Javea

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tol2kit.blogspot.com

Tol2kit

A multisite Gateway-based construction kit for Tol2 expression constructs. Welcome to the Tol2kit blog. This is a forum for discussing the Tol2kit. The Lawson lab's related system. Is a set of constructs that can be used with Invitrogen's multisite Gateway recombination-based cloning system to build expression constructs in a Tol2 transposon backbone (derived from constructs from the Kawakami lab. Saturday, February 22, 2014. Temporary Tol2kit wiki site. Thank you for your patience. New Tol2kit wiki URL.

tol2kit.genetics.utah.edu

Main Page - Tol2kit

Tol2kit v1.2, released November 2007. Generating stable transgenic zebrafish lines has historically been laborious, for three reasons:. Building transgenesis constructs by conventional subcloning can be difficult, especially when using long coding or regulatory sequences. Injecting plasmid DNA yields mosaic transient expression and low germline transgenesis rates. The Tol2kit uses site-specific recombination-based cloning ( multisite Gateway technology. It includes a variety of widely-useful entry clones.

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Canal Toledo

El tiempo en Toledo. Quién tiene prisa en aprobar un nuevo POM? El vigente plan de ordenación tiene 29 años de vida y como se ha demostrado con el pleito del IBI, los ciudadanos tienen pocas ganas y menos necesidad de que les recalifiquen sus tierras. Confianza por encima de los consejeros. El delegado será una figura política de primer orden y su función principal la de hacer reuniones. Y el lobo llegó. La reconstrucción de Castilla-La Mancha. Ertificado de Excelencia Turística 2015 de TripAdvisor.