tools.flycrispr.molbio.wisc.edu
CRISPR Optimal Target FinderThis tool finds all possible CRISPR targets of interest and quickly and clearly evaluates their specificity
http://tools.flycrispr.molbio.wisc.edu/
This tool finds all possible CRISPR targets of interest and quickly and clearly evaluates their specificity
http://tools.flycrispr.molbio.wisc.edu/
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CRISPR Optimal Target Finder | tools.flycrispr.molbio.wisc.edu Reviews
https://tools.flycrispr.molbio.wisc.edu
This tool finds all possible CRISPR targets of interest and quickly and clearly evaluates their specificity
Links – CRISPR fly design
http://www.crisprflydesign.org/links
CRISPR fly design Drosophila genome engineering. T7 endo I assay. ECRISPR target finder (Boutros lab). FlyCRISPR target finder (O’Connor-Giles lab). DRSC target finder (Drosophila RNAi Screening Center). Bloomington CRISPR fly stocks. FlyCRISPR Google discussion group. German Cancer Research Center (DKFZ). Division of Signaling and Functional Genomics. Im Neuenheimer Feld 580. F.port@dkfz.de. X6d;.boutros@dkfz.de. Sbullock-at-mrc-lmb.cam.ac.uk. Responsible for website content and design: Fillip Port.
BestGene Inc. - CRISPR Info
http://www.thebestgene.com/CRISPRInfoPage.do
Genome Editing Service, pls see here. For currently available Cas9 stocks at BestGene, pls see here. Random indels can be introduced into DSB via NHEJ. Currently, CRISPR/Cas9 has grown in popularity for Drosophila. In the application of CRISPR/Cas9 system in Drosophila. Genome editing, the two RNAs that play key roles can be synthesized into a single chimeric RNA (chiRNA or gRNA). By editing the 20 nt targeting sequence of gRNA, the system introduces DSB on the target sequence followed by an NGG or N...
Tools | flyCRISPR
http://www.flycrispr.molbio.wisc.edu/tools
Skip to main content. FlyCRISPR Optimal Target Finder. Is a web tool for identifying CRISPR target sites and evaluating their specificity using transparent rules based on empirical data in the literature so you can easily select the best target(s) for your project. The rules behind our algorithms are detailed in the user manual. And will be updated as new data becomes available. 4 Hsu, P.D. et al. DNA targeting specificity of RNA-guided Cas9 nucleases. Nat Biotechnol (2013). 6 Pattanayak, V. et al...
Protospacer - resources - Protospacer
http://www.protospacer.com/resources.html
Protospacer Workbench: CRISPR-Cas9 design. Http:/ www.genome-engineering.org/crispr/. A portal with excellent content on CRISPR. Https:/ www.addgene.org/CRISPR/guide/. A good guide to CRISPR, addgene also supplies links to a lot of other useful information/resources. How to Design Your gRNA for CRISPR Genome Editing. Http:/ www.nature.com/nbt/journal/v30/n9/full/nbt.2357.html. Comment on Jinek et al. 2012, A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. PubMed. A programma...
News & Results | flyCRISPR
http://www.flycrispr.molbio.wisc.edu/news
Skip to main content. Visit the flyCRISPR Discussion Group. For ongoing discussions. We'll also be posting new data and reagents here as they become available. June 10, 2015. Tools for scarless HDR with screenable markers. HDR with dsDNA donors offers mutliple advantages, including the ability to edit over large regions, insert large exogneous sequences, and incorporate selection markers. While selectable markers greatly facilitate identification of engineered organisms, most methods for removing mar...
CRISPR/Cas9系统_联合华益
http://www.unihuaii.com/Recruit/jiyinzubianji
Double-strand break, DSB),. Non-homologous end joining, NHEJ). Homologous recombination, HR). CRISPR/Cas (clustered regularly interspaced palindromic repeats/CRISPR associated,. DRSC Find CRISPR Tool. FlyCRISPR Optimal Target Finder. Dmelanogaster release 5, release 6,. Bassett AR, Tibbit C, Ponting CP, Liu JL (2013) Highly efficient targeted mutagenesis of. With the CRISPR/Cas9 system. Kondo S, Ueda R (2013) Highly improved gene targeting by germline-specific Cas9 expression in. Y[1] M{vas-Cas9.RFP-...
Links & References | flyCRISPR
http://www.flycrispr.molbio.wisc.edu/links
Skip to main content. Drosophila CRISPR target finders:. FlyCRISPR Optimal Target Finder. Zhang lab CRISPR website. Joung lab CRISPR website. Goldstein lab CRISPR website. Bassett, A.R., and Liu, J.L. (2014). CRISPR/Cas9 and Genome Editing in Drosophila. J Genet Genomics. Bassett, A.R., Tibbit, C., Ponting, C.P., and Liu, J.L. (2014). Mutagenesis and homologous recombination in Drosophila cell lines using CRISPR/Cas9. Biology open. Gratz, S.J., Wildonger, J., Harrison, M.M., and O'Con...Kondo, S., an...
Lab Links | O'Connor-Giles Laboratory
http://oconnorgiles.molbio.wisc.edu/lab-links
227C R.M. Bock Labs. Madison, WI 53706. 227C R.M. Bock Labs. Madison, WI 53706. Information and resources for CRISPR RNA/Cas9-mediated genome engineering in Drosophila. FlyCRISPR Target Finder tool. A web tool that identifies CRISPR target sites and evaluates their potential for off-target cleavage using transparent algorithms based on empirical studies in cell lines and animals. An active discussion board for posting questions and sharing tips on all things fly and CRISPR.
CRISPR/Cas9系统_联合华益
http://www.lianhehuayi.com/Recruit/jiyinzubianji
Double-strand break, DSB),. Non-homologous end joining, NHEJ). Homologous recombination, HR). CRISPR/Cas (clustered regularly interspaced palindromic repeats/CRISPR associated,. DRSC Find CRISPR Tool. FlyCRISPR Optimal Target Finder. Dmelanogaster release 5, release 6,. Bassett AR, Tibbit C, Ponting CP, Liu JL (2013) Highly efficient targeted mutagenesis of. With the CRISPR/Cas9 system. Kondo S, Ueda R (2013) Highly improved gene targeting by germline-specific Cas9 expression in. Y[1] M{vas-Cas9.RFP-...
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tools.flycrispr.molbio.wisc.edu
CRISPR Optimal Target Finder
Genome release 6 (the current FlyBase version) is now available for target design! Enter genomic DNA sequence to find CRISPR target sites:. Omit header lines and special characters.). Drosophila melanogaster (r 5/dm3). Drosophila melanogaster (r 6). Drosophila mauritiana (Dmau MS17). Drosophila ananassae (dana r1.3 FB2011 07). Drosophila erecta (dere r1.3 FB2011 08). Drosophila persimilis (dper r1.3 FB2010 02). Drosophila pseudoobscura (dpse r3.1 FB2013 03). Drosophila mojavensis (dmoj r1.3 FB2011 05).
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