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UNIVERSITY OF OTTAWA
LAURA TRINKLE-MULCAHY
451 ●●●●H RD
OT●●WA , ONTARIO, K1H 8M5
CANADA
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UNIVERSITY OF OTTAWA
LAURA TRINKLE-MULCAHY
451 ●●●●H RD
OT●●WA , ONTARIO, K1H 8M5
CANADA
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t r i n k l e l a b . c o m | trinklelab.com Reviews
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t r i n k l e l a b . c o m
http://www.trinklelab.com/overview.html
Aberrant phosphorylation has been linked to many human diseases, including diabetes and cancer, and my research studies the targeting of phosphatase complexes within living cells to identify and understand phosphorylation-mediated processes that can give rise to such diseases in humans. Genomic studies have provided a catalogue of protein kinases and phosphatases:. 90 phosphorylate Tyr residues. 107 dephosphorylate Tyr residues. 428 phosphorylate Ser/Thr residues. 40 phosphorylate Ser/Thr residues. Analy...
t r i n k l e l a b . c o m
http://www.trinklelab.com/imaging.html
Live Cell Time-Lapse Imaging. FRAP (fluorescence recovery after photobleaching). For qualitative FRAP, it may be enough to simply plot fluorescence intensity over time (known as the recovery curve), as a fraction of the initial fluorescence prior to photobleaching. Different pools of the same protein can be compared for relative mobility, or the same pool of protein can be assessed before and after a particular perturbation. FRET (fluorescence resonance energy transfer). This approach requires independen...
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http://www.trinklelab.com/publications.html
Mehta V, Trinkle-Mulcahy L. Recent advances in large-scale protein interactome mapping. F1000 Faculty Rev 5:782, 2016. Ard R, Mulatz K, Pomoransky JL, Parks RJ, Trinkle-Mulcahy L, Bell JC, Gee SH. Regulation of Macropinocytosis by Diacylglycerol Kinase ζ. PLoS One. 10:e0144942, 2015. Hennig S, Kong G, Mannen T, Sadowska A, Kobelke S, Blythe A, Knott GJ, Iyer KS, Ho D, Newcombe EA, Hosoki K, Goshima N, Kawaguchi T, Hatters D, Trinkle-Mulcahy. Platani M, Trinkle-Mulcahy L. Lam, YW, Trinkle-Mulcahy, L.
t r i n k l e l a b . c o m
http://www.trinklelab.com/people.html
Department of Cellular and Molecular Medicine. Ottawa, ON K1H 8M5. 1-613-562-5800 ext. 8068 (Laura) ext. 7397 (Lab). Ltrinkle @ uottawa . ca. Reseach in the lab is funded by the following organizations:. Michèle Prévost.
t r i n k l e l a b . c o m
http://www.trinklelab.com/proteomics.html
Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC). The main advantage of SILAC for labeling-based quantitative proteomics is that the control and experimental samples are differentially labeled upstream of processing steps that can introduce variability between them, such as SDS-PAGE separation, digestion and MS analysis. Isotopic amino acids introduce mass shifts into peptides that can be detected by MS analysis:. For our purposes, expressing the 3 mammalian PP1 isoforms as GFP-tagged prote...
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t r i n k l e l a b . c o m
Last Updated: July 2, 2015. Ottawa Institute of Systems Biology. Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Canada. Research funded by the following organizations:.
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Guest Rooms & Rates. Tucked away in the Blue Ridge Mountains of Southwestern Virginia and located just minutes from I-77 and I-81 about half-way between Charleston, West Virginia and Charlotte, North Carolina, this historic Wytheville, Virginia bed and breakfast. Indulge in our memorable, three-course breakfasts served fireside at a beautifully set table, complete with crystal and silver. All guestrooms. Romantic Date Nite Just the Two of You. Trinkle Mansion Bed and Breakfast. 525 W Main Street. Review ...
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