western-biologic.org
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Western Blot
This site will be reviewing western blot techniques and avaialble solutions. Currently reviewing products from advansta. Http:/ www.advansta.com).
闪晶生物-Western Blot检测-蛋白免疫印迹-1500.00/膜
实验对象为组织样品，取适量 250 500mg 新鲜组织样品或正确保存的组织样品，加1ml含蛋白酶抑制剂的总蛋白抽提试剂 或核蛋白抽提试剂 ，匀浆后抽提总蛋白 或核蛋白。 实验对象为细胞样品，每份样品取1 106 1 107细胞，PBS清洗细胞，去PBS加0.1ml 1ml含蛋白酶抑制剂的总蛋白抽提试剂 或核蛋白抽提试剂 抽提总蛋白 或核蛋白。 4 蛋白质转移到PVDF膜,按Bio-Rad蛋白转移装置说明组装滤纸凝胶纤维素夹层，30mA恒流条件下，4 C转移过夜。 6 western blot结果检测 化学发光法检测，膜与化学发光底物孵育，经X胶片曝光显影。 7 western blot数据分析 目的蛋白的灰度值除以内参GAPDH/Actin的灰度值以校正误差，所得结果代表某样品的目的蛋白相对含量。 联系 master@shinegene.org.cn shinegene@vip.163.com 电话 021-54460831-11. 2X Taq PCR Master Mix. Uracil DNA Glycosylase (UNG). Or shinegene@vip.163.com.
GenScript Western Blot Kit Overview
GenScript provides gene, peptide, protein, and antibody products and services to the international scientific community. GenScript specializes in custom gene and peptide synthesis, siRNA construction, and both polyclonal and monoclonal antibody production. Our state-of-the-art methodology incorporates meticulous design tools and a unified approach to synthesis that ensure our customers the highest quality results. What is a western blot? AN INNOVATIVE NEW WESTERN PROCEDURE. Quick and simple procedure.
GenScript Western Blot Kit Overview
GenScript provides gene, peptide, protein, and antibody products and services to the international scientific community. GenScript specializes in custom gene and peptide synthesis, siRNA construction, and both polyclonal and monoclonal antibody production. Our state-of-the-art methodology incorporates meticulous design tools and a unified approach to synthesis that ensure our customers the highest quality results. What is a western blot? AN INNOVATIVE NEW WESTERN PROCEDURE. Quick and simple procedure.
