XYLOME.VBI.VT.EDU

Poplar Biomass Interactome

Poplar Biomass Interactome Project. The genes investigated here with data on Y2H interactions, sequences, primers, etc. List of objectives from proposal. Overview of Y2H binary and cDNA library screens. The poplar biomass interactome project is a collaboration among the Department of Forest Resources and Environmental Conservation, the Department of Horticulture, and the Virginia Bioinformatics Institute at Virginia Tech. Journal articles generated by the poplar biomass interactome project.

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Poplar Biomass Interactome | xylome.vbi.vt.edu Reviews

<META> DESCRIPTION

Poplar Biomass Interactome Project. The genes investigated here with data on Y2H interactions, sequences, primers, etc. List of objectives from proposal. Overview of Y2H binary and cDNA library screens. The poplar biomass interactome project is a collaboration among the Department of Forest Resources and Environmental Conservation, the Department of Horticulture, and the Virginia Bioinformatics Institute at Virginia Tech. Journal articles generated by the poplar biomass interactome project.

<META> KEYWORDS

1 enter db

2 project personnel

3 general overview

4 protein interactions

5 objectives

6 yeast 2 hybrid screen

7 contact information

8 publications

9 usage policy

10 funding

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enter db,project personnel,general overview,protein interactions,objectives,yeast 2 hybrid screen,contact information,publications,usage policy,funding

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Poplar Biomass Interactome | xylome.vbi.vt.edu Reviews

https://xylome.vbi.vt.edu

Poplar Biomass Interactome Project. The genes investigated here with data on Y2H interactions, sequences, primers, etc. List of objectives from proposal. Overview of Y2H binary and cDNA library screens. The poplar biomass interactome project is a collaboration among the Department of Forest Resources and Environmental Conservation, the Department of Horticulture, and the Virginia Bioinformatics Institute at Virginia Tech. Journal articles generated by the poplar biomass interactome project.

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xylome.vbi.vt.edu

1

Poplar Biomass Interactome

http://xylome.vbi.vt.edu/Usage_Policy.html

The goal of our policy is to aid the progress of science by early release and also allow Poplar Biomass Interactome (Xylome) Project participants to reserve a reasonable period of time to publish on the data provided without concerns about preemption by other groups. We are providing interactome data at the cDNA library screen, interaction sequence tag (IST) level and in many cases at the binary screen/retest level ( http:/ xylome.vbi.vt.edu/Y2H Protocol.html. Or Amy Brunner ( abrunner@vt.edu.

2

Poplar Biomass Interactome

http://xylome.vbi.vt.edu/Y2H_Protocol.html

Overview of Y2H binary screen. Or filter paper overlay. Overview of Y2H cDNA library screen. Each DB-X fusion is tested for autoactivation capability on -His 3AT medium. Those that test positive for autoactivation are eliminated from further screening. Bait proteins (DB-X) and the AD fusion prey library (AD-prey) are co-transformed into yeast (Y8800) and plated on -His, 3AT medium from which obvious fast-growing colonies are selected.

3

Poplar Biomass Interactome

http://xylome.vbi.vt.edu/Renewal_Objectives.html

Objectives of Current Project. Perform functional analysis of selected pairs of interactors using transgenic poplar and Arabidopsis. Perform orthogonal tests of protein-protein interactions involving proteins selected for functional analysis. Expand Y2H screening of the xylem cDNA prey library using 200 new bait proteins. Maintain the project website for reporting new protein-protein interaction and functional interactomics data.

4

Publications

http://xylome.vbi.vt.edu/Publications.html

Poplar Biomass Interactome Project. Xia, R., B.C. Meyers, Z. Liu, E.P. Beers, S. Ye, Z. Liu, 2013. MicroRNA superfamilies descended from miR390 and their roles in secondary small interfering RNA biogenesis in eudicots. Plant Cell, 25:1555-72. Journal Link. Petzold, H.E., M. Zhao, E.P. Beers, 2012. Expression and function of proteases in vascular tissues. Physiol. Plant. 145:121-129 Journal Link. Brunner, am and E.P. Beers, 2010. Transcription factors in poplar growth and development. In P...

5

PBIP: List of 74 biomass ORFs screened against cDNA library or with binary-screen interactions.

http://xylome.vbi.vt.edu/ORF_List

PBIP: List of 74 biomass ORFs screened against cDNA library or with binary-screen interactions. Data available subject to this usage policy. Show all genes in target list. LocusID links to Details Page. Phytozome IDs link to Gbrowse. Arabidopsis IDs link to TAIR. Column headers allow sorting. Zinc finger, C2H2-type. Histidine phosphotransfer proteins (AHPs). Kinesin light chain related. Kinesin light chain related. Kinesin light chain related. InterPro domain Targeting for Xklp2. Zinc finger, RING-type.

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Poplar Biomass Interactome Project. The genes investigated here with data on Y2H interactions, sequences, primers, etc. List of objectives from proposal. Overview of Y2H binary and cDNA library screens. The poplar biomass interactome project is a collaboration among the Department of Forest Resources and Environmental Conservation, the Department of Horticulture, and the Virginia Bioinformatics Institute at Virginia Tech. Journal articles generated by the poplar biomass interactome project.

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