bio-ggs.blogspot.com
BioFreaks Biochemistry Blog (v.Beta): GGS LIVE - Cloning
https://bio-ggs.blogspot.com/2009/11/ggs-live-cloning.html
Sunday, 22 November 2009. GGS LIVE - Cloning. What is cloning you ask! I see cloning as set of methods which allow to copy specyfic piece of DNA and then to use it as we wish. Amplification of three different cDNAs (cDNA is a protein coding part of the gene for more informations see this amnimation McGraw Hill Animations - cDNA (complementary DNA). By PCR and transfering them (cloning) into first transient vector and afterwards to a final (target) vector. Each of our cDNAs is around 300bp. As you can see...
bio-ggs.blogspot.com
BioFreaks Biochemistry Blog (v.Beta): GGS LIVE - Antibody Generation
https://bio-ggs.blogspot.com/2015/05/ggs-live-antibody-generation.html
Saturday, 2 May 2015. GGS LIVE - Antibody Generation. Today in the GGS LIVE section we will cover how to generate rabbit polyclonal antibody:). Established methodology for antibody generation is an essential tool to study cellular functions of the protein of interest. Antibodies are generated by injection of the antigen. Into host animal (mouse, rat, rabbit etc) and subsequent isolation of antibodies from the animal serum. For instance from rabbit serum). Generation of antibody against protein X. Once we...
bio-ggs.blogspot.com
BioFreaks Biochemistry Blog (v.Beta): GGS LIVE - Protein purification from E.coli
https://bio-ggs.blogspot.com/2011/06/ggs-live-protein-purification-from.html
Thursday, 9 June 2011. GGS LIVE - Protein purification from E.coli. Yo Yo Biofreakers,. Today we are going to have closer look at the recombinant protein purification from bacterial. Purification of recombinant protein. Having an optimised protocol for protein purification is an essential tool to study properties of the protein of interest. Purification of GST-tagged chicken protein previously expressed in E.coli. Is an enzyme that transfer glutathione. We can purify it using one of the many protocols av...
biokimia45.blogspot.com
Kopi atau Bir? Pengaruh Kafein dan Alkohol pada Genom Kita ~ BioChemIsTry
http://biokimia45.blogspot.com/2013/12/kopi-atau-bir-pengaruh-kafein-dan.html
Thursday, December 19, 2013. Pengaruh Kafein dan Alkohol pada Genom Kita. Prof Martin Kupiec dan timnya di Departemen Bioteknologi dan Mikrobiologi Molekuler Universitas Tel Aviv telah menemukan bahwa minuman mungkin juga memiliki pengaruh yang berlainan pada genom kita. Penelitian ini dilakukan menggunakan 6000 galur khamir yang memiliki banyak kesamaan genetik penting dengan manusia. Antara kematian dan keabadian. Kanan: Proses pemanjangan telomer pada anti-aging. Subscribe to: Post Comments (Atom).
biokimia45.blogspot.com
Apa Itu Apoptosis? ~ BioChemIsTry
http://biokimia45.blogspot.com/2013/12/apa-itu-apoptosis.html
Tuesday, December 10, 2013. Tubuh manusia normal memiliki suatu mekanisme keseimbangan (homeo-stasis) antara pertumbuhan (proliferasi) dan kematian sel (cell death). Hal ini berguna untuk pertumbuhan normal. Bila salah satu keseimbangan terganggu akan menyebabkan suatu penyakit, misalnya bila pertumbuhan sel lebih cepat dari kematian sel maka akan menimbulkan Alzheimer dan penyakit parkison. Jaringan, dan proteksi terhadap patogen. AIF) yang menginduksi terjadinya apoptosis sel. Kromatin lanjut dan degra...
biokimia45.blogspot.com
Enzyme Kinetics Theory and Practice ~ BioChemIsTry
http://biokimia45.blogspot.com/2013/12/enzyme-kinetics-theory-and-practice.html
Saturday, December 21, 2013. Enzyme Kinetics Theory and Practice. Jika link tidak berfungsi, mohon hubungi melalui "Contact me" (side bar blog). Subscribe to: Post Comments (Atom). Tubuh manusia normal memiliki suatu mekanisme keseimbangan (homeo-stasis) antara pertumbuhan (proliferasi) dan kematian sel (cell death). Pembentukan Struktur Tiga Dimensi dengan Origami DNA. Harper's Illustrated Biochemistry (26th Ed, 2003). Chevening Scholarship, Beasiswa S-2 di UK. Cooking Up Membrane Proteins with BAM!
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Mengenal Cisplatin, Obat Kanker yang Berbasis Logam Platinum ~ BioChemIsTry
http://biokimia45.blogspot.com/2009/03/mengenal-cisplatin-obat-kanker-yang.html
Tuesday, March 3, 2009. Mengenal Cisplatin, Obat Kanker yang Berbasis Logam Platinum. Cisplatin atau cisplatinum atau. Diamminedichloroplatinum(II) adalah obat kemoterapi kanker yang berbasis logam platinum. Pada dasarnya senyawa turunan platinum yang menunjukkan antitumor/antikanker telah ribuan yang disintesis. Tetapi hanya 28 dari mereka yang telah diujicoba secara klinis dan hanya 2 yang sangat aktif yaitu cisplatin itu sendiri dan carboplatin. Diterima di seluruh dunia. Diterima di seluruh dunia.
bio-ggs.blogspot.com
BioFreaks Biochemistry Blog (v.Beta): GGS LIVE - Polymerase chain reaction (PCR)
https://bio-ggs.blogspot.com/2011/01/ggs-live-polymerase-chain-reaction-pcr.html
Wednesday, 26 January 2011. GGS LIVE - Polymerase chain reaction (PCR). Polymerase chain reaction (PCR). PCR allows for amplification of a particular DNA sequence. Ampliifcation of a gene X sequence from genomic DNA. Ok lets start. Polymerase chain reaction is a commonly used technique. The theory behind it as well aas practical part of PCR are very simple. For PCR reaction we need:. Thermostabile polymerase - which can stand temperature up to 95 degrees Celsius,. DNA template - contains target DNA,.
bio-ggs.blogspot.com
BioFreaks Biochemistry Blog (v.Beta): GGS LIVE - Protein expression in vertebrate cells
https://bio-ggs.blogspot.com/2012/08/ggs-live-protein-expression-in.html
Saturday, 25 August 2012. GGS LIVE - Protein expression in vertebrate cells. I am back BAYBE! Today we will cover a topic about expression of protein in vertebrate cells. Expression of protein X in human osteosarcoma cells (U2OS). Protein expression allows for either purification or study of its cellular function through for example its localisation etc. Expression of RFP-tagged-(Red Fluorescent Protein)-Protein X in U2OS cells to study its localisation. In our study case the U2OS cells will were transfe...