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Bordetellas Logbook:]

Group Members: Eriko Wong; Nurul Natasha; Nurul Nyzah; Ong Wendy; Siti Shahimah

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Bordetellas Logbook:] | bordetellas.blogspot.com Reviews
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Group Members: Eriko Wong; Nurul Natasha; Nurul Nyzah; Ong Wendy; Siti Shahimah
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8 immunofixation electrophoresis
9 steps involve
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Bordetellas Logbook:] | bordetellas.blogspot.com Reviews

https://bordetellas.blogspot.com

Group Members: Eriko Wong; Nurul Natasha; Nurul Nyzah; Ong Wendy; Siti Shahimah

INTERNAL PAGES

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1

Bordetellas Logbook:]: Human Pancreatic Polypeptide (PP)

http://bordetellas.blogspot.com/2009/10/human-pancreatic-polypeptide-pp.html

Group Members: Eriko Wong; Nurul Natasha; Nurul Nyzah; Ong Wendy; Siti Shahimah. Sunday, October 25, 2009. Human Pancreatic Polypeptide (PP). Human pancreatic polypeptide (PP) is a polypeptide hormone secreted by the pancreatic polypeptide (PP) cells of the islets of Langerhans in the endocrine portion of the pancreas. It consists of a chain of 36 amino acids. 1 Capture of human PP molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-PP antibody. 7 Quantificati...

2

Bordetellas Logbook:]: August 2009

http://bordetellas.blogspot.com/2009_08_01_archive.html

Group Members: Eriko Wong; Nurul Natasha; Nurul Nyzah; Ong Wendy; Siti Shahimah. Sunday, August 30, 2009. It's my turn again. So i'm going to write about a test done in micro lab. Wampole™ TOX A/B QUIK CHEK. This test is a rapid immunoassay for detecting Clostridium difficile toxins A and B. Suspected disease: C. difficile disease. Before proceeding with this test, we have to bring all reagents and devices to room temperature to ensure accurate results.). 3 Wash Membrane Device. 4 Add Substrate reagent.

3

Bordetellas Logbook:]: June 2009

http://bordetellas.blogspot.com/2009_06_01_archive.html

Group Members: Eriko Wong; Nurul Natasha; Nurul Nyzah; Ong Wendy; Siti Shahimah. Thursday, June 25, 2009. This is the summarised steps in performing UPCM. 1) Mix urine sample well. 2) Pour 10ml of urine sample into centrifuge tube. 3) Centrifuge the tube at 2000rpm for 10 minutes. 5) Vortexed the remaining volume of urine. 6) Load the sample into Med-Fuchs Rosenthal Counting Chamber. 7) Examine under microscope (40x). Subscribe to: Posts (Atom).

4

Bordetellas Logbook:]: Protein precipitation

http://bordetellas.blogspot.com/2009/09/protein-precipitation.html

Group Members: Eriko Wong; Nurul Natasha; Nurul Nyzah; Ong Wendy; Siti Shahimah. Sunday, September 13, 2009. As for my MP, i have to analyze a drug in plasma sample. However, there are many impurites in plasma sample. In order to obtain as little interfering peaks when analyzing by HPLC, we have to remove the protein in the plasma. General theory of using an organic solvent to precipitate out proteins in plasma:. Organic solvent reduces water activity. Thus water cannot dissolve the hydrophilic proteins.

5

Bordetellas Logbook:]: November 2009

http://bordetellas.blogspot.com/2009_11_01_archive.html

Group Members: Eriko Wong; Nurul Natasha; Nurul Nyzah; Ong Wendy; Siti Shahimah. Thursday, November 12, 2009. Ways to protect the column from damaging. Sorry for the super late post! Since the column of the HPLC is very expensive, we can have to do things to maximize the length of its life:). Here are the things to do:. During shutdown, always flush the column with ultrapure water to remove all the salts present from the buffer. If this is not done, salts will precipitate and cause clogging of column.

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med-scientist-in-action.blogspot.com med-scientist-in-action.blogspot.com

MedScientist: Immunochemistry- G6PD Quantitative Screening Test

http://med-scientist-in-action.blogspot.com/2009/08/immunochemistry-g6pd-quantitative.html

Today's science fiction is tomorrow's science fact. Monday, August 24, 2009. Immunochemistry- G6PD Quantitative Screening Test. Its my turn to post once again, woo! Today I’m gonna share with you guys, the Glucose-6-phosphate dehydrogenase (G6PD) screening test (Quantitative). By the OSMMR-D-D assay, which is a new machine currently under evaluation by the Immunochemistry lab. (which is the lab I’m permanently attached to.). End of story, good bye! Joanna Yeo, woo! MedScientists of Grp 6. Hope you are we...

med-scientist-in-action.blogspot.com med-scientist-in-action.blogspot.com

MedScientist: June 2009

http://med-scientist-in-action.blogspot.com/2009_06_01_archive.html

Today's science fiction is tomorrow's science fact. Tuesday, June 30, 2009. My 1st week of SIP in 2 Departments! This posting is for this week). My first week of attachment was filled with so much fun and laughter. I was posted to 2 departments. For a start,. Contents of my post:. 1) Briefly describing the daily routine work in Histology Department. 2) Operation of the Tissue Processor. 3) Principles of the common stains. 1) Organs and other specimens are received. 8) Slides were stained. Http:/ www....

med-scientist-in-action.blogspot.com med-scientist-in-action.blogspot.com

MedScientist: Transformation of chemically ultra-competent E.coli with plasmid DNA

http://med-scientist-in-action.blogspot.com/2009/09/transformation-of-chemically-ultra.html

Today's science fiction is tomorrow's science fact. Monday, September 21, 2009. Transformation of chemically ultra-competent E.coli with plasmid DNA. Posted by Vo Thu Hong Anh [Jess] 0705364H. To generate a large amount of plasmid DNA containing a desired gene, with the aid of chemically ultra-competent E.coli. Chemically ultra-competent E.coli. Cells will also be heated at 42. Place the paper disc containing plasmid DNA (commercially made and shipped) into a 1.5mL-centrifuge tube. On ice to thaw. After ...

med-scientist-in-action.blogspot.com med-scientist-in-action.blogspot.com

MedScientist: August 2009

http://med-scientist-in-action.blogspot.com/2009_08_01_archive.html

Today's science fiction is tomorrow's science fact. Monday, August 24, 2009. Immunochemistry- G6PD Quantitative Screening Test. Its my turn to post once again, woo! Today I’m gonna share with you guys, the Glucose-6-phosphate dehydrogenase (G6PD) screening test (Quantitative). By the OSMMR-D-D assay, which is a new machine currently under evaluation by the Immunochemistry lab. (which is the lab I’m permanently attached to.). End of story, good bye! Joanna Yeo, woo! MedScientists of Grp 6. The test in my ...

med-scientist-in-action.blogspot.com med-scientist-in-action.blogspot.com

MedScientist: November 2009

http://med-scientist-in-action.blogspot.com/2009_11_01_archive.html

Today's science fiction is tomorrow's science fact. Saturday, November 7, 2009. Done by: Janice Yeh. This is my second last department. Mainly observation of the work flow only.wasn't allow to do as i was only posted there for a week. 1) The following photos are taken with permission of the department supervisor. 2) Photos below may be abit disgusting. BE PREPARED! 1)When specimens are receieved, they are soaked fully in formalin to remove the smell and for preservation. Once the stain is done, cutting o...

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wedonknow.blogspot.com (:: G6PD qualitative screening test

http://wedonknow.blogspot.com/2009/09/g6pd-qualitative-screening-test.html

Wedonknow.blogspot.com (:. Wednesday, September 30, 2009. G6PD qualitative screening test. G6PD is a key enzyme in the hexose monophosphate pathway (HMP) and is required for the formation of NADPH. NADPH is essential for maintaining the integrity of the erythrocyte membrane. Specimen collection and preparation. Process specimen collected in EDTA tube. Before analysis, check that the specimen is not clotted. Performing G6PD screening test by R&D diagnostic G6PD deficiency screening test kit. With the same...

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wedonknow.blogspot.com (:: Immunochemistry- G6PD quantification

http://wedonknow.blogspot.com/2009/10/immunochemistry-g6pd-quantification.html

Wedonknow.blogspot.com (:. Monday, October 26, 2009. Hey guys, sorry for the late posting! This will be the last time I'm sharing! This time I am going to talk about the quantitative determination of G6PD on an analyzer, which is currently undergoing evaulation. Why do this test? An awareness of G6PD deficiency had been raised many years back in Singapore. Hence, all newborns will be screened for this deficiency. And of course, the samples used for this test will be all cord blood samples. TG02 - Group 8.

med-scientist-in-action.blogspot.com med-scientist-in-action.blogspot.com

MedScientist: IMMUNOCHEMISTRY-RUBELLA IgG TEST

http://med-scientist-in-action.blogspot.com/2009/09/immunochemistry-rubella-igg-test.html

Today's science fiction is tomorrow's science fact. Wednesday, September 30, 2009. Its my turn again. i'm gonna share with you one of the test that is carried out by the Architect i2000SR immunoassay. The Rubella IgG test. It is measure colourmetrically as RLUs and this RLUs detected by optical system is directly proportional to concentration of rubella Ab present in serum. The serum left is kept for 1 year at -70 to -90 degrees celsius for traceability. =). Done by: Joanna Yeo. Admin No. 0702054H. This ...

med-scientist-in-action.blogspot.com med-scientist-in-action.blogspot.com

MedScientist: Bone Marrow Aspiration!

http://med-scientist-in-action.blogspot.com/2009/10/bone-marrow-aspiration.html

Today's science fiction is tomorrow's science fact. Tuesday, October 6, 2009. I will discuss with all of you about Bone Marrow Aspiration (BMA) and the respective tests done. First, what is BMA? It is the removal of a small amount of bone marrow, which is the soft tissue in liquid form inside the bones (place where blood cells formed; hollow part of most bones) for examination. Why do we need to do BMA? Where is BMA obtain from? How is BMA obtain? Traplok Bone Marrow Biopsy Needle (blue T-shape needle is...

med-scientist-in-action.blogspot.com med-scientist-in-action.blogspot.com

MedScientist: Slide-making

http://med-scientist-in-action.blogspot.com/2009/09/slide-making.html

Today's science fiction is tomorrow's science fact. Sunday, September 13, 2009. Greetings to everyone, :). I was posted to Cytogenetics Lab. In this lab, I was given the opportunity to culture my own cells and perform karyotyping. It was very fun but quite scary in a way. The scary part was during karyotyping for my own chromosomes as I did not know what would be the result. Initially I was worried about the outcome but overall, it was not too bad. J. Contents of my post. Mitotic Index is the number of m...

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Bordetellas Logbook:]

Group Members: Eriko Wong; Nurul Natasha; Nurul Nyzah; Ong Wendy; Siti Shahimah. Thursday, November 12, 2009. Ways to protect the column from damaging. Sorry for the super late post! Since the column of the HPLC is very expensive, we can have to do things to maximize the length of its life:). Here are the things to do:. During shutdown, always flush the column with ultrapure water to remove all the salts present from the buffer. If this is not done, salts will precipitate and cause clogging of column.

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