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Wedonknow.blogspot.com (:. Wednesday, November 4, 2009. Hello, my final blog posting will on the disposable Dengue test kit from HUMAN(Company name). Dengue is a flavivirus transmitted in the tropics and involves both humans abd Aedes mosquitos. The 4 serotypes of dengue (DEN-1, -2, -3 and -4) are similar, but are antigenically different. This implies that infection with one of the serotypes only provides cross-protective immunity for a few of months. Thursday, October 29, 2009. 7 pound the tissue using ...

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Wedonknow.blogspot.com (:. Wednesday, November 4, 2009. Hello, my final blog posting will on the disposable Dengue test kit from HUMAN(Company name). Dengue is a flavivirus transmitted in the tropics and involves both humans abd Aedes mosquitos. The 4 serotypes of dengue (DEN-1, -2, -3 and -4) are similar, but are antigenically different. This implies that infection with one of the serotypes only provides cross-protective immunity for a few of months. Thursday, October 29, 2009. 7 pound the tissue using ...

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wedonknow.blogspot.com (: | wedonknow.blogspot.com Reviews

https://wedonknow.blogspot.com

Wedonknow.blogspot.com (:. Wednesday, November 4, 2009. Hello, my final blog posting will on the disposable Dengue test kit from HUMAN(Company name). Dengue is a flavivirus transmitted in the tropics and involves both humans abd Aedes mosquitos. The 4 serotypes of dengue (DEN-1, -2, -3 and -4) are similar, but are antigenically different. This implies that infection with one of the serotypes only provides cross-protective immunity for a few of months. Thursday, October 29, 2009. 7 pound the tissue using ...

INTERNAL PAGES

wedonknow.blogspot.com

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wedonknow.blogspot.com (:: RNA extraction from tissues

http://wedonknow.blogspot.com/2009/10/rna-extraction-from-tissues.html

Wedonknow.blogspot.com (:. Thursday, October 29, 2009. RNA extraction from tissues. Procedures for RNA extraction from tissues:. 1 pre-weigh an empty cryovial. 2 take the tissue specimen out from the cryovial and place it on a 100mm petri dish. 3 use a scapel and sterican (like fork and knife) to cut the tissue into desired size. 4 transfer the tissue into the pre-weigh cryovial and weigh again (this will give the exact weigh of the tissue being process). 6 add 650µl of RLT buffer into the cryovial.

2

wedonknow.blogspot.com (:: CK-MB test

http://wedonknow.blogspot.com/2009/10/ck-mb-test.html

Wedonknow.blogspot.com (:. Sunday, October 4, 2009. Creatine kinase (CK) is a dimeric enzyme which occurs in four different fours: a mitochondrial isoenzyme and CK-MM( muscle type), CK-BB (brain type) and CKMB. The determination of CKMB is an important element in the diagnosis of myocardiac ischemia. CKMB is detectable in the blood about 3-8 hours after the onset of cardiac symptoms and can be detectable over a period of time which is dependant on the course of the condition. TG02 - Group 8. Like, any fa...

3

wedonknow.blogspot.com (:: Dengue Test

http://wedonknow.blogspot.com/2009/11/dengue-test.html

Wedonknow.blogspot.com (:. Wednesday, November 4, 2009. Hello, my final blog posting will on the disposable Dengue test kit from HUMAN(Company name). Dengue is a flavivirus transmitted in the tropics and involves both humans abd Aedes mosquitos. The 4 serotypes of dengue (DEN-1, -2, -3 and -4) are similar, but are antigenically different. This implies that infection with one of the serotypes only provides cross-protective immunity for a few of months. November 8, 2009 at 11:02 PM. TG02 - Group 8. March 1...

4

wedonknow.blogspot.com (:: August 2009

http://wedonknow.blogspot.com/2009_08_01_archive.html

Wedonknow.blogspot.com (:. Wednesday, August 26, 2009. Hello, i am Stanley and this is my second blog posting. Part of my experiment will include the use of MTT assay to determine both the growth curve of both normal lung cell (MRC-5) and large lung carcinoma cells (H460) which are seeded in a 96 well flat bottom plate. The advantages of using MTT will include high accuracy and the exclusion of using radioisotope. Below are steps for performing the MTT proliferation assay:. Preparation of MTT stock.

5

wedonknow.blogspot.com (:: October 2009

http://wedonknow.blogspot.com/2009_10_01_archive.html

Wedonknow.blogspot.com (:. Thursday, October 29, 2009. RNA extraction from tissues. Procedures for RNA extraction from tissues:. 1 pre-weigh an empty cryovial. 2 take the tissue specimen out from the cryovial and place it on a 100mm petri dish. 3 use a scapel and sterican (like fork and knife) to cut the tissue into desired size. 4 transfer the tissue into the pre-weigh cryovial and weigh again (this will give the exact weigh of the tissue being process). 6 add 650µl of RLT buffer into the cryovial.

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med-scientist-in-action.blogspot.com

MedScientist: Immunochemistry- G6PD Quantitative Screening Test

http://med-scientist-in-action.blogspot.com/2009/08/immunochemistry-g6pd-quantitative.html

Today's science fiction is tomorrow's science fact. Monday, August 24, 2009. Immunochemistry- G6PD Quantitative Screening Test. Its my turn to post once again, woo! Today I’m gonna share with you guys, the Glucose-6-phosphate dehydrogenase (G6PD) screening test (Quantitative). By the OSMMR-D-D assay, which is a new machine currently under evaluation by the Immunochemistry lab. (which is the lab I’m permanently attached to.). End of story, good bye! Joanna Yeo, woo! MedScientists of Grp 6. Hope you are we...

med-scientist-in-action.blogspot.com

MedScientist: June 2009

http://med-scientist-in-action.blogspot.com/2009_06_01_archive.html

Today's science fiction is tomorrow's science fact. Tuesday, June 30, 2009. My 1st week of SIP in 2 Departments! This posting is for this week). My first week of attachment was filled with so much fun and laughter. I was posted to 2 departments. For a start,. Contents of my post:. 1) Briefly describing the daily routine work in Histology Department. 2) Operation of the Tissue Processor. 3) Principles of the common stains. 1) Organs and other specimens are received. 8) Slides were stained. Http:/ www....

med-scientist-in-action.blogspot.com

MedScientist: Transformation of chemically ultra-competent E.coli with plasmid DNA

http://med-scientist-in-action.blogspot.com/2009/09/transformation-of-chemically-ultra.html

Today's science fiction is tomorrow's science fact. Monday, September 21, 2009. Transformation of chemically ultra-competent E.coli with plasmid DNA. Posted by Vo Thu Hong Anh [Jess] 0705364H. To generate a large amount of plasmid DNA containing a desired gene, with the aid of chemically ultra-competent E.coli. Chemically ultra-competent E.coli. Cells will also be heated at 42. Place the paper disc containing plasmid DNA (commercially made and shipped) into a 1.5mL-centrifuge tube. On ice to thaw. After ...

med-scientist-in-action.blogspot.com

MedScientist: August 2009

http://med-scientist-in-action.blogspot.com/2009_08_01_archive.html

Today's science fiction is tomorrow's science fact. Monday, August 24, 2009. Immunochemistry- G6PD Quantitative Screening Test. Its my turn to post once again, woo! Today I’m gonna share with you guys, the Glucose-6-phosphate dehydrogenase (G6PD) screening test (Quantitative). By the OSMMR-D-D assay, which is a new machine currently under evaluation by the Immunochemistry lab. (which is the lab I’m permanently attached to.). End of story, good bye! Joanna Yeo, woo! MedScientists of Grp 6. The test in my ...

med-scientist-in-action.blogspot.com

MedScientist: Bone Marrow Aspiration!

http://med-scientist-in-action.blogspot.com/2009/10/bone-marrow-aspiration.html

Today's science fiction is tomorrow's science fact. Tuesday, October 6, 2009. I will discuss with all of you about Bone Marrow Aspiration (BMA) and the respective tests done. First, what is BMA? It is the removal of a small amount of bone marrow, which is the soft tissue in liquid form inside the bones (place where blood cells formed; hollow part of most bones) for examination. Why do we need to do BMA? Where is BMA obtain from? How is BMA obtain? Traplok Bone Marrow Biopsy Needle (blue T-shape needle is...

bordetellas.blogspot.com

Bordetellas Logbook:]: July 2009

http://bordetellas.blogspot.com/2009_07_01_archive.html

Group Members: Eriko Wong; Nurul Natasha; Nurul Nyzah; Ong Wendy; Siti Shahimah. Tuesday, July 28, 2009. Hey Siti Shahimah is here again. :). Okay, so I have learnt about TDx/IMX. Basically, TDx/IMX are the name of analyzers that help to detect therapeutic dosage of drugs so as to ensure appropriate therapy for patients. TDx analyzes drugs such as cyclosporine, methotrexate and everolimus. Cyclosporine and everolimus are immunosuppresant drugs whereas methotrexate is an antineoplastic drug. 150 uL of pat...

bordetellas.blogspot.com

Bordetellas Logbook:]: September 2009

http://bordetellas.blogspot.com/2009_09_01_archive.html

Group Members: Eriko Wong; Nurul Natasha; Nurul Nyzah; Ong Wendy; Siti Shahimah. Saturday, September 26, 2009. For hematology section, tests such as full blood count (FBC), erythrocyte sedimentation rate ESR), peripheral blood films (PBF), and retic count are done. there are also malaria tests and dengue panel. Sunday, September 13, 2009. General theory of using an organic solvent to precipitate out proteins in plasma:. Organic solvent reduces water activity. This is done by:. 5 Vortex for 2 minutes.

bordetellas.blogspot.com

Bordetellas Logbook:]: blood collection

http://bordetellas.blogspot.com/2009/10/blood-collection.html

Group Members: Eriko Wong; Nurul Natasha; Nurul Nyzah; Ong Wendy; Siti Shahimah. Sunday, October 11, 2009. In this post, I am going to write about a tutorial given by one of our colleague. All of us have to attend this tutorials unless we are busy at our work stations. These tutorials are given at least once every 2 weeks. Topic for today: blood collection. Times to provide thorough mixing of the additives. Also, it is important to note that shaking of blood tubes may cause hemolysis of the blood. They c...

bordetellas.blogspot.com

Bordetellas Logbook:]: ways to protect the column from damaging

http://bordetellas.blogspot.com/2009/11/ways-to-protect-column-from-damaging.html

Group Members: Eriko Wong; Nurul Natasha; Nurul Nyzah; Ong Wendy; Siti Shahimah. Thursday, November 12, 2009. Ways to protect the column from damaging. Sorry for the super late post! Since the column of the HPLC is very expensive, we can have to do things to maximize the length of its life:). Here are the things to do:. During shutdown, always flush the column with ultrapure water to remove all the salts present from the buffer. If this is not done, salts will precipitate and cause clogging of column.

bordetellas.blogspot.com

Bordetellas Logbook:]: Immunofixation Electrophoresis

http://bordetellas.blogspot.com/2009/10/immunofixation-electrophoresis.html

Group Members: Eriko Wong; Nurul Natasha; Nurul Nyzah; Ong Wendy; Siti Shahimah. Friday, October 16, 2009. 1 Proteins in the samples (urine and serum) are separated by electrophoresis on agarose gel. 2 Immunofixation of electrophoresed proteins includes, the monospecific antisera diffuse into. Agarose gel and bind to the antigens (proteins), if present. Thus, resulting in the precipitation. 3 The unprecipitated antigens are removed from the gel by blotting and washing. Of the fractions is dark in colour.

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wedonknow.blogspot.com

wedonknow.blogspot.com (:

Wedonknow.blogspot.com (:. Wednesday, November 4, 2009. Hello, my final blog posting will on the disposable Dengue test kit from HUMAN(Company name). Dengue is a flavivirus transmitted in the tropics and involves both humans abd Aedes mosquitos. The 4 serotypes of dengue (DEN-1, -2, -3 and -4) are similar, but are antigenically different. This implies that infection with one of the serotypes only provides cross-protective immunity for a few of months. Thursday, October 29, 2009. 7 pound the tissue using ...

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