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RNA-seq Analysis
http://nextgenseek.com/category/rna-seq-analysis
Making Sense of Next-Gen Sequencing Data. You are here: Home. Archives for RNA-seq Analysis. Kallisto paper summary: Near-optimal RNA-seq quantification. May 31, 2015. Middot; 1 Comment. Middot; Tagged With: kallisto. Bayesian Approach to Single-Cell RNA-Seq Differential Expression Analysis. June 12, 2014. Middot; 1 Comment. Middot; Tagged With: DE in Single-Cell RNA-seq. July 25, 2013. Middot; Leave a Comment. Recently just started playing with TopHat, after a while back, for doing gene expression quant...
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How to run kallisto on NCBI SRA RNA-Seq data for differential expression using the mac terminal | genophoria
https://genophoria.wordpress.com/2015/05/13/how-to-run-kallisto-on-ncbi-sra-rna-seq-data-for-differential-expression-using-the-mac-terminal
Genomics euphoria: ramblings of a scientist on genetics, genomics and the meaning of life. How to run kallisto on NCBI SRA RNA-Seq data for differential expression using the mac terminal. On May 13, 2015. Very useful post on Kallisto. This post explains how to run the exceptionally fast RNA-seq k-mer aligner kallisto. From the Pachter lab on data you download from NCBI’s Short Read Archive. Kallisto is really fast and non-memory intensive, without sacrificing accuracy (at least, according to their paper.
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Letters from the trenches of war on cancer (Part I) | genophoria
https://genophoria.wordpress.com/2014/07/15/letters-from-the-trenches-of-war-on-cancer-part-i
Genomics euphoria: ramblings of a scientist on genetics, genomics and the meaning of life. Letters from the trenches of war on cancer (Part I). On July 15, 2014. It is not news to anyone that I study the biology and regulation of RNA (see the two previous posts on this very blog: here. And Noam who published their work back in 2008. And with the help of my genius friend Hamed. We developed, benchmarked and validated a computational method named TEISER. Given the success of miRNA regulation and my previou...
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RNA rises | genophoria
https://genophoria.wordpress.com/2014/03/23/the-rise-of-rna
Genomics euphoria: ramblings of a scientist on genetics, genomics and the meaning of life. On March 23, 2014. Roushkin et al. 2013, Nature. Last year the first ever large scale binding information for a compendium of RNA-binding proteins (RBPs) was published ( Ray et al, 2013, Nature. The computational methods are also gaining, from SeqFold ( Ouyang et al, 2013, Genome res. To our TEISER ( Goodarzi et al, 2012, Nature. Did I mention these are exciting times? Which I encourage you to read. Interesting...
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Sailfish
http://nextgenseek.com/tag/sailfish
Making Sense of Next-Gen Sequencing Data. You are here: Home. Kallisto paper summary: Near-optimal RNA-seq quantification. May 31, 2015. Middot; 1 Comment. Middot; Tagged With: kallisto. Kallisto, a new ultra fast RNA-seq quantitation method. May 6, 2015. Middot; 2 Comments. Middot; Tagged With: kallisto. Transcript quantitation from RNA-seq. RNA-Skim: new RNA-seq quantitation method that is faster than Sailfish. June 19, 2014. Middot; 2 Comments. This is a quick post: summary/thoughts* on the new paper ...
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Salmon
http://nextgenseek.com/category/rna-seq/salmon
Making Sense of Next-Gen Sequencing Data. You are here: Home. Paper Summary: Fast and accurate single-cell RNA-seq analysis using equivalence class counts. January 17, 2016. Middot; Leave a Comment. Filed Under: equivalent class counts. Middot; Tagged With: equivalent class counts for single-cell RNA-seq analysis. Kallisto paper summary: Near-optimal RNA-seq quantification. May 31, 2015. Middot; 1 Comment. Middot; Tagged With: kallisto. Follow me on Twitter. How Does NextSeq 500 Compare With MiSeq and Hi...
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Seq Papers
http://nextgenseek.com/seq-papers
Making Sense of Next-Gen Sequencing Data. You are here: Home. One of the goals of this blog is to frequently write blog posts on interesting papers on sequencing, be it a new sequencing technology/assay or a new computational method to analyze sequencing data. Although have not really managed to do that to my satisfaction, here is an attempt to summarize what is done so far. Hope this will help increase more posts on interesting papers. Seq Papers in 2016. Seq Papers in 2015. Seq Papers in 2014. Sailfish...
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Interesting papers from the GTEx (Genotype-Tissue Expression) Project
http://nextgenseek.com/2015/05/three-papers-from-the-gtex-genotype-tissue-expression-project
Making Sense of Next-Gen Sequencing Data. You are here: Home. Interesting papers from the GTEx (Genotype-Tissue Expression) Project. Interesting papers from the GTEx (Genotype-Tissue Expression) Project. May 8, 2015. Middot; Leave a Comment. It has been a good week for RNA-seq enthusiasts. First, Kallisto. A new way to quantify transcript expression, happened at BOG15. And this weeks’ Science magazine has three interesting papers on the GTEx (Genotype-Tissue Expression) Project. The human transcriptome a...
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Functional impacts of RNA modifications | genophoria
https://genophoria.wordpress.com/2015/08/30/functional-impacts-of-rna-modifications
Genomics euphoria: ramblings of a scientist on genetics, genomics and the meaning of life. Functional impacts of RNA modifications. On August 30, 2015. My good friend and all-around awesome scientist Claudio has been working on this very cool idea the fruits of which is now available to all. I had the pleasure of being on his team and I’m personally fascinated by the problem. The broad question that Claudio is tackling is “what to RNA modifications do in the cell? And I invite everyone to read this paper.
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