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Medical Juniors' "LogBook"

Sunday, November 8, 2009. Very sorry for the late post. Cytology laboratory has 2 different departments; Gynaecology and Non-Gynaecology department. Non-Gynae deals with all kinds of liquid specimens that come from different part of the body, for example; knee aspirate, sputum, urine, FNA fluid from the breast, thyroid or parotid, and pleural fluid. Thus, as intern, i wasnt allow to handle these potential biohazardous materials except for my own urine, and sputum. Specimens that comes in vials (ThinPrep ...

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Medical Juniors' "LogBook" | doctor-in-the-making.blogspot.com Reviews
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Sunday, November 8, 2009. Very sorry for the late post. Cytology laboratory has 2 different departments; Gynaecology and Non-Gynaecology department. Non-Gynae deals with all kinds of liquid specimens that come from different part of the body, for example; knee aspirate, sputum, urine, FNA fluid from the breast, thyroid or parotid, and pleural fluid. Thus, as intern, i wasnt allow to handle these potential biohazardous materials except for my own urine, and sputum. Specimens that comes in vials (ThinPrep ...
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1 medical juniors' logbook
2 cytology lab
3 nadiah bte sukma
4 hi again
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8 staining
9 results
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Medical Juniors' "LogBook" | doctor-in-the-making.blogspot.com Reviews

https://doctor-in-the-making.blogspot.com

Sunday, November 8, 2009. Very sorry for the late post. Cytology laboratory has 2 different departments; Gynaecology and Non-Gynaecology department. Non-Gynae deals with all kinds of liquid specimens that come from different part of the body, for example; knee aspirate, sputum, urine, FNA fluid from the breast, thyroid or parotid, and pleural fluid. Thus, as intern, i wasnt allow to handle these potential biohazardous materials except for my own urine, and sputum. Specimens that comes in vials (ThinPrep ...

INTERNAL PAGES

doctor-in-the-making.blogspot.com doctor-in-the-making.blogspot.com
1

Medical Juniors' "LogBook": July 2009

http://doctor-in-the-making.blogspot.com/2009_07_01_archive.html

Thursday, July 23, 2009. Week 5: Histopathology Lab. It is now my turn to share the experiences ive gained within this 5 week of very-tiring-yet-fun SIP. First, i have to explain the different parts of the working area. According to our schedule we'll be posted to 4 months of Histopathology lab and a month of Cytology. In Histo lab, there are;. Reception/ Specimen sorting room. Staining (H&E and Special Stains). Sorting slides Verification of patient's form Signed out to the respectives pathologists.

2

Medical Juniors' "LogBook": November 2009

http://doctor-in-the-making.blogspot.com/2009_11_01_archive.html

Sunday, November 8, 2009. Very sorry for the late post. Cytology laboratory has 2 different departments; Gynaecology and Non-Gynaecology department. Non-Gynae deals with all kinds of liquid specimens that come from different part of the body, for example; knee aspirate, sputum, urine, FNA fluid from the breast, thyroid or parotid, and pleural fluid. Thus, as intern, i wasnt allow to handle these potential biohazardous materials except for my own urine, and sputum. Specimens that comes in vials (ThinPrep ...

3

Medical Juniors' "LogBook": June 2009

http://doctor-in-the-making.blogspot.com/2009_06_01_archive.html

Monday, June 29, 2009. Week 1: Microbiology Dept. Name: Siti Hawa Bte Azali. Admin No.: 0704087F. During the 1st week of my attachment , i was assigned to work in the Microbiology Department where specimens are tested for presence of any pathogenic organisms and where identification of causative agents were carried out. This section is important as it aids in the final confirmation of clinical diagnosis. This test is done on a patient's serum( in a plain tube) by testing it against 11 different reagents ...

4

Medical Juniors' "LogBook": August 2009

http://doctor-in-the-making.blogspot.com/2009_08_01_archive.html

Sunday, August 30, 2009. Medical Microbiology - Virology Serology Laboratory Techniques. Low Wei Qi Kenneth TG02. This time im going to share another serology technique in detecting viruses total antibody for Influenza A, Influenza B, Herpes Simplex and many others. The test is known as Complement Fixation Test ( CFT). This test uses the properties of complement to lyse cells in the presence of antigen-antibody complexes. Sooo here is the STEP BY STEP:. 50 ul of specimen is added to the first well. The p...

5

Medical Juniors' "LogBook": October 2009

http://doctor-in-the-making.blogspot.com/2009_10_01_archive.html

Sunday, October 25, 2009. Tng Wess Lee, 0702570C. Confirmation of Mband (Identifying Artefacts in SPE). M-band, is a protein which is either immunoglobin or immunoglobin light chains. It will form a distinct band often at the gamma region. M-band is due to excessive production of immunoglobins due to diseases such as multiple myeloma, Monoclonal Gammopathy of Undetermined Significance, chronic lymphocytic leukemia, etc. We therefore run immunofixation (IFE) to identify the immunoglobin class of the M-band.

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med-scientist-in-action.blogspot.com med-scientist-in-action.blogspot.com

MedScientist: Immunochemistry- G6PD Quantitative Screening Test

http://med-scientist-in-action.blogspot.com/2009/08/immunochemistry-g6pd-quantitative.html

Today's science fiction is tomorrow's science fact. Monday, August 24, 2009. Immunochemistry- G6PD Quantitative Screening Test. Its my turn to post once again, woo! Today I’m gonna share with you guys, the Glucose-6-phosphate dehydrogenase (G6PD) screening test (Quantitative). By the OSMMR-D-D assay, which is a new machine currently under evaluation by the Immunochemistry lab. (which is the lab I’m permanently attached to.). End of story, good bye! Joanna Yeo, woo! MedScientists of Grp 6. Hope you are we...

med-scientist-in-action.blogspot.com med-scientist-in-action.blogspot.com

MedScientist: June 2009

http://med-scientist-in-action.blogspot.com/2009_06_01_archive.html

Today's science fiction is tomorrow's science fact. Tuesday, June 30, 2009. My 1st week of SIP in 2 Departments! This posting is for this week). My first week of attachment was filled with so much fun and laughter. I was posted to 2 departments. For a start,. Contents of my post:. 1) Briefly describing the daily routine work in Histology Department. 2) Operation of the Tissue Processor. 3) Principles of the common stains. 1) Organs and other specimens are received. 8) Slides were stained. Http:/ www....

med-scientist-in-action.blogspot.com med-scientist-in-action.blogspot.com

MedScientist: Transformation of chemically ultra-competent E.coli with plasmid DNA

http://med-scientist-in-action.blogspot.com/2009/09/transformation-of-chemically-ultra.html

Today's science fiction is tomorrow's science fact. Monday, September 21, 2009. Transformation of chemically ultra-competent E.coli with plasmid DNA. Posted by Vo Thu Hong Anh [Jess] 0705364H. To generate a large amount of plasmid DNA containing a desired gene, with the aid of chemically ultra-competent E.coli. Chemically ultra-competent E.coli. Cells will also be heated at 42. Place the paper disc containing plasmid DNA (commercially made and shipped) into a 1.5mL-centrifuge tube. On ice to thaw. After ...

med-scientist-in-action.blogspot.com med-scientist-in-action.blogspot.com

MedScientist: August 2009

http://med-scientist-in-action.blogspot.com/2009_08_01_archive.html

Today's science fiction is tomorrow's science fact. Monday, August 24, 2009. Immunochemistry- G6PD Quantitative Screening Test. Its my turn to post once again, woo! Today I’m gonna share with you guys, the Glucose-6-phosphate dehydrogenase (G6PD) screening test (Quantitative). By the OSMMR-D-D assay, which is a new machine currently under evaluation by the Immunochemistry lab. (which is the lab I’m permanently attached to.). End of story, good bye! Joanna Yeo, woo! MedScientists of Grp 6. The test in my ...

bordetellas.blogspot.com bordetellas.blogspot.com

Bordetellas Logbook:]: July 2009

http://bordetellas.blogspot.com/2009_07_01_archive.html

Group Members: Eriko Wong; Nurul Natasha; Nurul Nyzah; Ong Wendy; Siti Shahimah. Tuesday, July 28, 2009. Hey Siti Shahimah is here again. :). Okay, so I have learnt about TDx/IMX. Basically, TDx/IMX are the name of analyzers that help to detect therapeutic dosage of drugs so as to ensure appropriate therapy for patients. TDx analyzes drugs such as cyclosporine, methotrexate and everolimus. Cyclosporine and everolimus are immunosuppresant drugs whereas methotrexate is an antineoplastic drug. 150 uL of pat...

bordetellas.blogspot.com bordetellas.blogspot.com

Bordetellas Logbook:]: September 2009

http://bordetellas.blogspot.com/2009_09_01_archive.html

Group Members: Eriko Wong; Nurul Natasha; Nurul Nyzah; Ong Wendy; Siti Shahimah. Saturday, September 26, 2009. For hematology section, tests such as full blood count (FBC), erythrocyte sedimentation rate ESR), peripheral blood films (PBF), and retic count are done. there are also malaria tests and dengue panel. Sunday, September 13, 2009. General theory of using an organic solvent to precipitate out proteins in plasma:. Organic solvent reduces water activity. This is done by:. 5 Vortex for 2 minutes.

bordetellas.blogspot.com bordetellas.blogspot.com

Bordetellas Logbook:]: blood collection

http://bordetellas.blogspot.com/2009/10/blood-collection.html

Group Members: Eriko Wong; Nurul Natasha; Nurul Nyzah; Ong Wendy; Siti Shahimah. Sunday, October 11, 2009. In this post, I am going to write about a tutorial given by one of our colleague. All of us have to attend this tutorials unless we are busy at our work stations. These tutorials are given at least once every 2 weeks. Topic for today: blood collection. Times to provide thorough mixing of the additives. Also, it is important to note that shaking of blood tubes may cause hemolysis of the blood. They c...

bordetellas.blogspot.com bordetellas.blogspot.com

Bordetellas Logbook:]: ways to protect the column from damaging

http://bordetellas.blogspot.com/2009/11/ways-to-protect-column-from-damaging.html

Group Members: Eriko Wong; Nurul Natasha; Nurul Nyzah; Ong Wendy; Siti Shahimah. Thursday, November 12, 2009. Ways to protect the column from damaging. Sorry for the super late post! Since the column of the HPLC is very expensive, we can have to do things to maximize the length of its life:). Here are the things to do:. During shutdown, always flush the column with ultrapure water to remove all the salts present from the buffer. If this is not done, salts will precipitate and cause clogging of column.

bordetellas.blogspot.com bordetellas.blogspot.com

Bordetellas Logbook:]: Immunofixation Electrophoresis

http://bordetellas.blogspot.com/2009/10/immunofixation-electrophoresis.html

Group Members: Eriko Wong; Nurul Natasha; Nurul Nyzah; Ong Wendy; Siti Shahimah. Friday, October 16, 2009. 1 Proteins in the samples (urine and serum) are separated by electrophoresis on agarose gel. 2 Immunofixation of electrophoresed proteins includes, the monospecific antisera diffuse into. Agarose gel and bind to the antigens (proteins), if present. Thus, resulting in the precipitation. 3 The unprecipitated antigens are removed from the gel by blotting and washing. Of the fractions is dark in colour.

med-scientist-in-action.blogspot.com med-scientist-in-action.blogspot.com

MedScientist: Bone Marrow Aspiration!

http://med-scientist-in-action.blogspot.com/2009/10/bone-marrow-aspiration.html

Today's science fiction is tomorrow's science fact. Tuesday, October 6, 2009. I will discuss with all of you about Bone Marrow Aspiration (BMA) and the respective tests done. First, what is BMA? It is the removal of a small amount of bone marrow, which is the soft tissue in liquid form inside the bones (place where blood cells formed; hollow part of most bones) for examination. Why do we need to do BMA? Where is BMA obtain from? How is BMA obtain? Traplok Bone Marrow Biopsy Needle (blue T-shape needle is...

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Medical Juniors' "LogBook"

Sunday, November 8, 2009. Very sorry for the late post. Cytology laboratory has 2 different departments; Gynaecology and Non-Gynaecology department. Non-Gynae deals with all kinds of liquid specimens that come from different part of the body, for example; knee aspirate, sputum, urine, FNA fluid from the breast, thyroid or parotid, and pleural fluid. Thus, as intern, i wasnt allow to handle these potential biohazardous materials except for my own urine, and sputum. Specimens that comes in vials (ThinPrep ...

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