e-crisp.org
E-CRISP Design
http://www.e-crisp.org/E-CRISP/index.html
Indicate which reference genome E-CRISP should find targets in? Indices are pre-built for each organisms individual gene sequences. Separated list of official gene symbols or Accession IDs. For e.g. PFKP;PFKFB3;PRKCQ;CAMK1D (for Human). ENSDARG00000090752;ENSDARG00000063570;ENSDARG00000074914;ENSDARG00000032801 (for Zebrafish). FBgn0000442;FBgn0250906;FBgn0020618;FBgn0044323 (for Fly). One or more Fasta formatted sequence. For e.g:. Transcript IDs (ENST.) can be entered in subsection 4. Choose between di...
e-crisp.org
E-CRISP-Evaluation
http://www.e-crisp.org/E-CRISP/reannotate_crispr.html
Which reference genome should the designs target? Indices are pre-built for each organism's individual gene sequences. Enter one or more. FASTA format sequences to evaluate e.g:. The file should contain. FASTA format sequences to evaluate e.g:. Should the CRISPR designs should be checked for any secondary off-target effects. This means if they will cut in any exogenous, foreign introduced sequences. Select individual off-targets from the list or paste a set of fasta sequences into the text area. Populus ...
e-crisp.org
E-CRISP Help
http://www.e-crisp.org/E-CRISP/aboutpage.html
Go to this page to design your custom CRISPR guide RNAs. Design of CRISPR constructs. Florian Heigwer, Marco Breinig, Tianzuo Zhan, Michael Boutros. Programming: Grainne Kerr, Oliver Dreier, Johanna Kratzer, Pauline Burkhardt, Alexander Mattausch, Pelz Oliver. Ideas: Marco Breinig, Tianzuo Zhan, Jan Winter, Dirk Brüggemann. Heigwer, F. , Kerr, G. and Boutros, M. E-CRISP: fast CRISPR target site identification. Nat Methods 11, 122-123 (2014). In this section, the user can specify the experimental purpose ...
e-crisp.org
E-CRISP Links
http://www.e-crisp.org/E-CRISP/links.html
Design of CRISPR constructs. E-TALEN is a web service to design TALENs for introducing knock-out mutations, for endogenous tagging and targeted excision repair. The tool can be used to design TALENs against a single target but also for up to 50 target genes in parallel. E-TALEN guides the user through end-to-end de-novo design process for specific sequences or genomic loci. In addition, it can also be used to evaluate existing TALEN designs. Visit E-TALEN: www.e-talen.org/E-TALEN. For suggestions please ...
