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E-CRISP Design

Indicate which reference genome E-CRISP should find targets in? Indices are pre-built for each organisms individual gene sequences. Separated list of official gene symbols or Accession IDs. For e.g. PFKP;PFKFB3;PRKCQ;CAMK1D (for Human). ENSDARG00000090752;ENSDARG00000063570;ENSDARG00000074914;ENSDARG00000032801 (for Zebrafish). FBgn0000442;FBgn0250906;FBgn0020618;FBgn0044323 (for Fly). One or more Fasta formatted sequence. For e.g:. Transcript IDs (ENST.) can be entered in subsection 4. Choose between di...

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E-CRISP Design | e-crisp.org Reviews
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Indicate which reference genome E-CRISP should find targets in? Indices are pre-built for each organisms individual gene sequences. Separated list of official gene symbols or Accession IDs. For e.g. PFKP;PFKFB3;PRKCQ;CAMK1D (for Human). ENSDARG00000090752;ENSDARG00000063570;ENSDARG00000074914;ENSDARG00000032801 (for Zebrafish). FBgn0000442;FBgn0250906;FBgn0020618;FBgn0044323 (for Fly). One or more Fasta formatted sequence. For e.g:. Transcript IDs (ENST.) can be entered in subsection 4. Choose between di...
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6 e crisp
7 design
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E-CRISP Design | e-crisp.org Reviews

https://e-crisp.org

Indicate which reference genome E-CRISP should find targets in? Indices are pre-built for each organisms individual gene sequences. Separated list of official gene symbols or Accession IDs. For e.g. PFKP;PFKFB3;PRKCQ;CAMK1D (for Human). ENSDARG00000090752;ENSDARG00000063570;ENSDARG00000074914;ENSDARG00000032801 (for Zebrafish). FBgn0000442;FBgn0250906;FBgn0020618;FBgn0044323 (for Fly). One or more Fasta formatted sequence. For e.g:. Transcript IDs (ENST.) can be entered in subsection 4. Choose between di...

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e-crisp.org e-crisp.org
1

E-CRISP Design

http://www.e-crisp.org/E-CRISP/index.html

Indicate which reference genome E-CRISP should find targets in? Indices are pre-built for each organisms individual gene sequences. Separated list of official gene symbols or Accession IDs. For e.g. PFKP;PFKFB3;PRKCQ;CAMK1D (for Human). ENSDARG00000090752;ENSDARG00000063570;ENSDARG00000074914;ENSDARG00000032801 (for Zebrafish). FBgn0000442;FBgn0250906;FBgn0020618;FBgn0044323 (for Fly). One or more Fasta formatted sequence. For e.g:. Transcript IDs (ENST.) can be entered in subsection 4. Choose between di...

2

E-CRISP-Evaluation

http://www.e-crisp.org/E-CRISP/reannotate_crispr.html

Which reference genome should the designs target? Indices are pre-built for each organism's individual gene sequences. Enter one or more. FASTA format sequences to evaluate e.g:. The file should contain. FASTA format sequences to evaluate e.g:. Should the CRISPR designs should be checked for any secondary off-target effects. This means if they will cut in any exogenous, foreign introduced sequences. Select individual off-targets from the list or paste a set of fasta sequences into the text area. Populus ...

3

E-CRISP Help

http://www.e-crisp.org/E-CRISP/aboutpage.html

Go to this page to design your custom CRISPR guide RNAs. Design of CRISPR constructs. Florian Heigwer, Marco Breinig, Tianzuo Zhan, Michael Boutros. Programming: Grainne Kerr, Oliver Dreier, Johanna Kratzer, Pauline Burkhardt, Alexander Mattausch, Pelz Oliver. Ideas: Marco Breinig, Tianzuo Zhan, Jan Winter, Dirk Brüggemann. Heigwer, F. , Kerr, G. and Boutros, M. E-CRISP: fast CRISPR target site identification. Nat Methods 11, 122-123 (2014). In this section, the user can specify the experimental purpose ...

4

E-CRISP Links

http://www.e-crisp.org/E-CRISP/links.html

Design of CRISPR constructs. E-TALEN is a web service to design TALENs for introducing knock-out mutations, for endogenous tagging and targeted excision repair. The tool can be used to design TALENs against a single target but also for up to 50 target genes in parallel. E-TALEN guides the user through end-to-end de-novo design process for specific sequences or genomic loci. In addition, it can also be used to evaluate existing TALEN designs. Visit E-TALEN: www.e-talen.org/E-TALEN. For suggestions please ...

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konscience.de konscience.de

KNS029 Knusper, Knusper, CRISPR! Wer editiert meine DNA? | KonScience

http://www.konscience.de/2015/06/kns029-knusper-knusper-crispr-wer-editiert-meine-dna

Der Wissenschaftspodcast aus Konstanz. Impressum, Datenschutz & Disclaimer. KNS029 Knusper, Knusper, CRISPR! Wer editiert meine DNA? Hier ist Musik drinne! Bakterieller Virenscanner, Züchtungsrevoluzzer und Genomeditor uvm. Software is eating the world? CRISPR will eat the world! Wenn Dir diese Episode unseres kleinen Podcast gefallen hat (oder unsere archivierten. Bewerte uns bitte bei iTunes. Henry @ KNS029 Knusper, Knusper, CRISPR! Wer editiert meine DNA? Katrin @ KNS029 Knusper, Knusper, CRISPR!

blog-biosyn.com blog-biosyn.com

Web resources for the CRISPR Cas System! – Bio-Synthesis, Inc.

https://blog-biosyn.com/2014/01/15/web-resources-for-the-crispr-cas-system

Custom Peptide Synthesis, Custom Antibody , Custom DNA, Custom RNA ,Custom BNA COMMITED TO BIOMIC RESEARCH since 1984. Web resources for the CRISPR Cas System! Web resources for the CRISPR Cas System! By Klaus D. Linse. January 15, 2014. Bull; ( 1. Http:/ www.oxfcrispr.org. Oxford Fly CRISPR Resources. Http:/ www.crisprflydesign.org/. Offers Cas9 transgenic stocks. Http:/ www.flyrnai.org/crispr/. A web tool to identify CRISPRs for fly study. Http:/ flycrispr.molbio.wisc.edu/. Crass: The CRISPR Assembler.

crisprflydesign.org crisprflydesign.org

Links – CRISPR fly design

http://www.crisprflydesign.org/links

CRISPR fly design Drosophila genome engineering. T7 endo I assay. ECRISPR target finder (Boutros lab). FlyCRISPR target finder (O’Connor-Giles lab). DRSC target finder (Drosophila RNAi Screening Center). Bloomington CRISPR fly stocks. FlyCRISPR Google discussion group. German Cancer Research Center (DKFZ). Division of Signaling and Functional Genomics. Im Neuenheimer Feld 580. F.port@dkfz.de. X6d;.boutros@dkfz.de. Sbullock-at-mrc-lmb.cam.ac.uk. Responsible for website content and design: Fillip Port.

e-crisp-test.dkfz.de e-crisp-test.dkfz.de

E-CRISP Links

http://e-crisp-test.dkfz.de/E-CRISP/links.html

This page is old and discontinoued. Plase use www.e-crisp.org for research. E-TALEN is a web service to design TALENs for introducing knock-out mutations, for endogenous tagging and targeted excision repair. The tool can be used to design TALENs against a single target but also for up to 50 target genes in parallel. E-TALEN guides the user through end-to-end de-novo design process for specific sequences or genomic loci. In addition, it can also be used to evaluate existing TALEN designs.

protospacer.com protospacer.com

Protospacer - resources - Protospacer

http://www.protospacer.com/resources.html

Protospacer Workbench: CRISPR-Cas9 design. Http:/ www.genome-engineering.org/crispr/. A portal with excellent content on CRISPR. Https:/ www.addgene.org/CRISPR/guide/. A good guide to CRISPR, addgene also supplies links to a lot of other useful information/resources. How to Design Your gRNA for CRISPR Genome Editing. Http:/ www.nature.com/nbt/journal/v30/n9/full/nbt.2357.html. Comment on Jinek et al. 2012, A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. PubMed. A programma...

igtrcn.org igtrcn.org

CRISPR/Cas Web Resources : IGTRCN

http://igtrcn.org/knowledgebase/crisprcas-web-resources

Insect Genetic Technologies Service Providers. 2015 Technical Workshop – Album. Web Resources for design and planning of CRISPR/Cas9 studies. From Bassett and Liu (2014) CRISPR/Cas9 and Genome Editing in Drosophila. J. Genetics and Genomics 41, 7-19 doi.10.1016/j.gg.2013.12.004. Http:/ www.oxfcrispr.org/. Oxford Fly CRISPR Resources. Bassett et al., 2013. Http:/ www.crisprflydesign.org/. Offers Cas9 transgenic stocks. DRSC CRISPR finder (Perrimon Lab). Http:/ www.flyrnai.org/crispr/. Ren et al., 2013.

flycrispr.molbio.wisc.edu flycrispr.molbio.wisc.edu

Links & References | flyCRISPR

http://www.flycrispr.molbio.wisc.edu/links

Skip to main content. Drosophila CRISPR target finders:. FlyCRISPR Optimal Target Finder. Zhang lab CRISPR website. Joung lab CRISPR website. Goldstein lab CRISPR website. Bassett, A.R., and Liu, J.L. (2014). CRISPR/Cas9 and Genome Editing in Drosophila. J Genet Genomics. Bassett, A.R., Tibbit, C., Ponting, C.P., and Liu, J.L. (2014). Mutagenesis and homologous recombination in Drosophila cell lines using CRISPR/Cas9. Biology open. Gratz, S.J., Wildonger, J., Harrison, M.M., and O'Con...Kondo, S., an...

gepg.hms.harvard.edu gepg.hms.harvard.edu

GEPG Background

http://gepg.hms.harvard.edu/GEPG-background.php

The Genome Engineering Production Group. At Harvard Medical School. Background: CRISPRs and TALENs. Gene knockout by TALE or CRISPR nucleases. CRISPR Genome Engineering Resources (MIT):. Http:/ www.genome-engineering.org/crispr/. DRSC CRISPR Tool (Drosophila RNAi Screening Center at Harvard Medical School):. Http:/ www.flyrnai.org/crispr2/. Http:/ www.e-crisp.org/E-CRISP/. Http:/ www.e-talen.org/E-TALEN/. SIFTED: prediction of TALE DNA binding specificities including potential off-targets (BWH/Bulyk lab).

ccv.med.harvard.edu ccv.med.harvard.edu

Center for Causal Consequences of Variation

http://ccv.med.harvard.edu/resources.htm

The CCV Center has ended. As of 10/28/2015 this website will no longer be updated. Center for Causal Consequences of Variation. Review of Methods and Tools. Our Commitment to Diversity. How CCV Supports NIH Goals. Our Four Specific Aims. Review of Methods and Tools. Our Commitment to Diversity. Human and mouse exome CRISPR sites (2014, three Cas9 proteins). Human exome CRISPR sites (2013). Church lab CRISPR/Cas materials. Joung lab CRISPR/Cas materials. Joung lab Genome Engineering Newsgroup.

zgenebio.com.tw zgenebio.com.tw

CRISPR / Cas9 原理與應用 - 力鈞生物科技有限公司  ZGENEBIO BIOTECH INC.

http://www.zgenebio.com.tw/crispr--cas9-2140729702332872503329992.html

21147;鈞生物科技有限公司 ZGENEBIO BIOTECH INC. 38364;於我們. 23526;驗天地. TALEN 載體構築服務. CRISPR 載體構築服務. CRISPR / Cas9 原理與應用. CRISPR 植物載體構築服務. CRISPR / TALEN 基因編輯技術說明會線上報名. TALEN / CRISPR Zebrafish Gene KO Services. 31361;變篩選服務與產品. CRISPR/TALEN 線上訂購. CRISPR/TALEN target site design. 36681;錄組定序. Small RNA 定序. LncRNA 定序服務. 34920;達諎定序. 22522;因體定序. 21934;細胞全基因組定序. RAD-Seq 簡化基因組測序. 20154;類基因體定序. NGS Library prep Kit for Illumina. 24314;庫專用試劑. DNA / CirDNA / RNA KIT. ZGeneBio Plasma or Serum Circulating Nucleic Acid Extraction.

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E-CRISP Start

Go to this page to design your custom CRISPR guide RNAs. Design of CRISPR constructs. E-CRISP is a software tool to design and evaluate target sites for use with the Clustered Regularly Interspaced Short Palindromic Repeat ( CRISPR. Heigwer, F. , Kerr, G. and Boutros, M. , E-CRISP: fast CRISPR target site identification. Nat. Methods 11, 122-123 (2014). link. Major Database upgrade, featuring more organisms and updated genome releases. More information can be found at HELP. 3 Additional organisms have be...

e-crisp.org e-crisp.org

E-CRISP Design

Indicate which reference genome E-CRISP should find targets in? Indices are pre-built for each organisms individual gene sequences. Separated list of official gene symbols or Accession IDs. For e.g. PFKP;PFKFB3;PRKCQ;CAMK1D (for Human). ENSDARG00000090752;ENSDARG00000063570;ENSDARG00000074914;ENSDARG00000032801 (for Zebrafish). FBgn0000442;FBgn0250906;FBgn0020618;FBgn0044323 (for Fly). One or more Fasta formatted sequence. For e.g:. Transcript IDs (ENST.) can be entered in subsection 4. Choose between di...

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