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Rolf Lutz
Schil●●●●●tr. 4
Rue●●●eim , 76761
DE
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Rolf Lutz
Schil●●●●●tr. 4
Rue●●●eim , 76761
DE
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EXPRESSYS - References
http://www.expressys.com/main_references.html
The following includes selected links and publications describing applications of the pZ Expression System in various fields of basic and applied research. A complete reference list can be found at e.g. Google Scholar. Quantitative control and analysis of gene activities. Brewster RC et al. (2014). The Transcription FactorTitration Effect Dictates Level of Gene Expression. Cell, doi:10.1016/j.cell.2014.02.022 ( link. Golding I and Cox EC (2004). RNA dynamics in live. Independent control of gene activities.
EXPRESSYS - Order Information
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For further information, please, contact:. Tel 49 6221 54 6721. Fax 49 6221 54 6718. 11 These Conditions shall apply to every sale made or agreed to be made by Expressys. No order given by the Buyer shall constitute an agreement for sale unless accepted by Expressys on these Conditions. Any Conditions submitted proposed or stipulated by the Buyer in whatever form, whether written or oral, are expressly waived and excluded. 33 Expressys' liability under condition 3.2 shall be limited to replacing the ...
EXPRESSYS - Tools & Reagents
http://www.expressys.com/main_tools.html
The following components of the pZ Expression System are available:. 11 Vectors for Cloning and Expression. Data sheet (.pdf). Overview of available vectors. 12 Vectors for chromosomal Integration. DeoR, supE44, Delta(lacZYA-argFV169), Phi80 lacZDeltaM15, hsdR17(rK- mK ), recA1, endA1, gyrA96, thi-1, relA1. DeoR, supE44, Delta(lacZYA-argFV169), Phi80 lacZDeltaM15, hsdR17(rK- mK ), recA1, endA1, gyrA96, thi-1, relA1. LacY1, leuB6, mcrB , supE44, thi-1, thr-1, tonA21. Cloning and Expression Vectors:. Bull;...
EXPRESSYS - pZ Vectors
http://www.expressys.com/main_vectors.html
1 Architecture of the pZ vector system. The pZ vector family comprises three main modules which are in general separated by the unique (see Notes for exceptions) cleavage sites XhoI, XbaI, AvrII, SpeI, SacI and AatII . Gene, or the luciferase. Gene The latter two are intended to be repalced by the customers gene of interest (GOI). Chromosome carry the attachment site of phage Lambda (attP) in addition. Bull; The GOI (gene of interest) inserted in the pZ vectors is. Bull; EcoRI is not unique in vectors c...
EXPRESSYS - pZ Host Strains
http://www.expressys.com/main_strains.html
Zi strains carry at their attB. Locus two copies of the laci. Gene encoding the Lac Repressor. Z1 strains carry at their attB. Locus two copies of Lac Repressor and Tet Repressor encoding genes ( laci. Respectively) driven by the constitutive promoters P. The genes encoding the regulatory proteins are accompanied by a gene conferring resistance to Spectinomycin (Sp. Transfer of the regulatory module to other strains can be accomplished via general transduction, e.g. by phage P1. Due to its low transforma...
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Team:Cambridge - 2008.igem.org
http://2008.igem.org/Team:Cambridge
From 2008.igem.org. Since the emergence of Synthetic Biology. Our project sets the foundation for future research in engineered multi-cellularity by pursuing electrical and peptide signalling, and cellular self-differentiation through spontaneous spatial patterning. Using the amino acid glutamate as our 'neurotransmitter', we have successfully demonstrated a voltage response in bacterial cells. This is a first step in the direction of engineering multicellular behaviour.
IGEM:Cambridge/2008 - OpenWetWare
http://openwetware.org/wiki/IGEM:Cambridge/2008
This year, the Cambridge iGEM team is working towards creating an integrated Bacterial Recombinant Artificial Intelligence Network (iBRAIN). Our concept is to model eukaryotic neural behaviour using populations of bacteria. We are looking at two main aspects of this concept: self-organisation using Turing pattern formation, and synaptic signal transduction using voltage output glutamate detection (read more.). Poster and Presentation Outline. Add a Lab Notebook. This page has been accessed 14,876 times.
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expressyrself77 | Just another WordPress.com site
Just another WordPress.com site. Haha Life.kicks me in the ballz everytime XD. A moment of weakness, all barriers down, the moment all feelings have been bottled up and is about to spill. I haven’t updated this blog in a long time, I started out with the enthusiasm of a child, but now left with the energy of a frail person. I’m going to take this opportunity to rant as twitter is no longer my sanctuary. Man that was really dark XD. I’m so damn tireddd…off to bed! You all know it :/. MY BEST FRIEND :D.
EXPRESSYS - Tools for Gene Expression
EXPRESSYS offers tools and reagents for gene expression in prokaryotic hosts based on the pZ expression system developed by. Dr Rolf Lutz and Prof. Hermann Bujard (. Lutz and Bujard, 1997. The system consists of the following components:. For cloning and expression. A versatile vector system of modular structure offers freely interchangeable functional units:. 149; 5 tightly and independently controllable promoters. 149; 5 different selection markers. 149; 4 origins of replication. 149; 2 cloning sites.
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