Gene trapping is a high-throughput approach that is used to introduce insertional mutations across the genome in mouse embryonic stem (ES) cells. In addition to generating standard loss-of-function alleles, newer gene trap vectors offer a variety of post-insertional modification strategies for the generation of other experimental alleles. IGTC cell lines in database:. All news and events.
http://www.genetrap.org/
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IGTC, International Gene Trap Consortium | genetrap.org Reviews
https://genetrap.org
Gene trapping is a high-throughput approach that is used to introduce insertional mutations across the genome in mouse embryonic stem (ES) cells. In addition to generating standard loss-of-function alleles, newer gene trap vectors offer a variety of post-insertional modification strategies for the generation of other experimental alleles. IGTC cell lines in database:. All news and events.
genetrap.org
IGTC, International Gene Trap Consortium
https://www.genetrap.org/info/software.html
IGTC, International Gene Trap Consortium
https://www.genetrap.org/info/links.html
Centre for Modelling Human Disease. Embryonic Stem Cell Database. University of Manitoba, Canada). Exchangeable Gene Trap Clones. Kumamoto University, Japan). German Gene Trap Consortium. Sanger Institute Gene Trap Resource. Soriano Lab Gene Trap Database. Fred Hutchinson Cancer Research Center, Seattle, USA). National Center for Biotechnology Information. Mutant Mouse Regional Resource Centers. Ensembl Mouse Genome Server. Genome Survey Sequence Database. Entrez cross database search.
IGTC, International Gene Trap Consortium
https://www.genetrap.org/dataaccess/browse.html
IGTC, International Gene Trap Consortium
https://www.genetrap.org/tutorials/locate.html
Tutorials: Locating A Cell Line. Locating a gene trap cell line in a gene/locus of interest. This tutorial demonstrates how to locate a gene trap cell line in a gene/locus of interest. For information on the gene trapping process, please see our overview tutorial. Browse Biological Pathways or Gene Ontology categories. Browse Ensembl or UCSC Genome Browsers. To begin a search, select Data Access. In the menu bar at the top of the page. Scroll down to select the type of search you wish to perform. For fur...
IGTC, International Gene Trap Consortium
https://www.genetrap.org/info/faq.html
What is a gene trap? Gene trapping creates random insertional mutations in the genome that are immediately accessible to molecular characterization. Gene trap vectors contain a promoterless reporter gene and/or selectable marker which is activated upon insertion within an endogenous gene, hence, "gene trapping". For more information, see the Gene Trap Tutorial. How much does a gene trap cell line cost? What is the best way to search the IGTC for a match to my gene? If you are looking for specific identif...
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From Disease Genes to Protein Pathways :: DiGtoP
http://www.digtop.de/tools/collaborations.php
National Genome Research Network Website. Helmholtz Alliance for Mental Health in an Ageing Society Website. EUCOMM - European Conditional Mouse Mutagenesis Program Website. EuTRACC - European Transcriptome, Regulome and Cellular Commitment Consortium Website. EuMMCR - European Mouse Mutant Cell Repository Website. EMMA - The European Mouse Mutant Archive Website. IKMC - International Knockout Mouse Consortium Website. IGTC - International Gene Trap Consortium Website.
Targeting
http://ko.cwru.edu/services/targeting.shtml
What You Will Provide Part I. A map of the targeting strategy showing the selectable markers, arm lengths, and deletion length. The map should clearly indicate the linearization site. Arrange a meeting with us to discuss the targeting strategy. What We Will Do Part I. We will provide you with ES cell DNA. What You Will Provide Part II. An autoradiogram of a genomic Southern of ES cell genomic DNA cut with your diagnostic enzyme and probed with your external probe. What We Will Do Part II. You will cut th...
CMHD - ENU Mutagenesis & Physiology
http://www.cmhd.ca/enu_mutagenesis/index.html
TCP Research and Support -. TCP Research and Facility Operations. THE TORONTO CENTRE FOR PHENOGENOMICS. CENTRE FOR MODELING HUMAN DISEASE. TCP News and Events. Mutagenesis and Physiology Overview. Search Mutants by Phenotype. Search Mutants by Heritability. Physiology Assays and Protocols. View our full list of databases. 2004 Toronto Centre for Phenogenomics.
CMHD - Gene Trap
http://www.cmhd.ca/genetrap/index.html
TCP Research and Support -. TCP Research and Facility Operations. THE TORONTO CENTRE FOR PHENOGENOMICS. CENTRE FOR MODELING HUMAN DISEASE. TCP News and Events. Welcome to the Centre for Modeling Human Disease (CMHD) Gene Trap Resource – the PolyA Trap Resource of the International Gene Trap Consortium (IGTC). In which researchers are able to submit search criteria. Researchers will receive automated e-mail notification of matching gene trap clones as they are entered into the library and database. Canada...
CMHD - Gene Trap - Personnel
http://www.cmhd.ca/genetrap/personnel.html
TCP Research and Support -. TCP Research and Facility Operations. THE TORONTO CENTRE FOR PHENOGENOMICS. CENTRE FOR MODELING HUMAN DISEASE. TCP News and Events. CMHD Gene Trap Facility Members. William L. Stanford, Ph.D. – Director. Audrey I. Shukalyuk, Ph.D. – Postdoctoral Fellow. Laury Nutter, Ph.D. – NorCOMM Project Manager. Christine To, M.Sc. Programmer. Tammy Reid, M.Sc. – Head Technician. Qing Lan, M.D. – Tissue Culture Technician. Helen Zheng, M.Sc. – Molecular Biology Technician.
CMHD - Search Mutants by Phenotype
http://cmhd.mshri.on.ca/enu_mutagenesis/mice_reports/mice_reports_phenotype.asp
TCP Research and Support -. TCP Research and Facility Operations. THE TORONTO CENTRE FOR PHENOGENOMICS. CENTRE FOR MODELING HUMAN DISEASE. TCP News and Events. Search Mutants By Phenotype:. Muscular, Neurological and Behavior. Hematology and Blood Smear. Bone Density and Tissue Composition. 2004 Toronto Centre for Phenogenomics.
CMHD - Gene Trap - Primer
http://cmhd.mshri.on.ca/genetrap/primer.htm
TCP Research and Support -. TCP Research and Facility Operations. THE TORONTO CENTRE FOR PHENOGENOMICS. CENTRE FOR MODELING HUMAN DISEASE. TCP News and Events. The gene trap vector is introduced into ES cells by electroporation or retroviral infection and clones containing gene trap insertions are selected by antibiotic resistance. Antibiotic resistant clones are then picked into 96-well master plates, which are later replicated to generate frozen stocks for long-term storage of clones, cell lysates ...
CMHD - Gene Trap - Clone Request
http://cmhd.mshri.on.ca/genetrap/clone_request_info.html
TCP Research and Support -. TCP Research and Facility Operations. THE TORONTO CENTRE FOR PHENOGENOMICS. CENTRE FOR MODELING HUMAN DISEASE. TCP News and Events. Important Information to Consider Before Ordering Clones. Before ordering your clones, we strongly recommend that you review the RACE trace file and blast the gene trap sequence tag against the USCS genome browser. And/or NCBI mouse genome. To make the best choice of the clones. 2004 Toronto Centre for Phenogenomics.
CMHD - Sitemap
http://www.cmhd.ca/sitemap.html
TCP Research and Support -. TCP Research and Facility Operations. THE CENTRE FOR PHENOGENOMICS. CENTRE FOR MODELING HUMAN DISEASE. TCP News and Events. ENU Mutagenesis and Physiology. Search Mutants by Phenotype. Search Mutants by Heritability. Physiology and Mutagenesis Overview. Physiology Assays and Protocols. TCP News and Events. 2004 The Centre for Phenogenomics.
CMHD - Gene Trap - Collaborators
http://cmhd.mshri.on.ca/genetrap/collaborators.htm
TCP Research and Support -. TCP Research and Facility Operations. THE TORONTO CENTRE FOR PHENOGENOMICS. CENTRE FOR MODELING HUMAN DISEASE. TCP News and Events. 2004 Toronto Centre for Phenogenomics.
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Trapper Home Page
Cold Spring Harbor Laboratory. Enter ET or GT line:. Welcome to the Arabidopsis Genetrap Website at Cold Spring Harbor Lab. This website describes a collection of transposon insertions and their flanking sequences from the Martienssen lab. A major goal of the NSF Arabidopsis 2010 project is to characterize the roles and functions of unknown and hypothetical genes. The TRAPPER collection currently contains putative insertions in over 1500 of these undescribed genes. Here. Is a listing of these genes.
Trapper Home Page
Cold Spring Harbor Laboratory. Enter ET or GT line:. Welcome to the Arabidopsis Genetrap Website at Cold Spring Harbor Lab. This website describes a collection of transposon insertions and their flanking sequences from the Martienssen lab. A major goal of the NSF Arabidopsis 2010 project is to characterize the roles and functions of unknown and hypothetical genes. The TRAPPER collection currently contains putative insertions in over 1500 of these undescribed genes. Here. Is a listing of these genes.
GGTC German Gene Trap Consortium
2910 new clones released. Clones are generated with enhanced gene trap vectors. 8247 new gene trap sequence tags released. Release of the new GGTC web interface. New release of GGTC gene trap library:. More than 35000 gene trap sequence tags analyzed by genomic PCR. The German Genetrap Consortium (GGTC). Generates a reference library of gene trap sequence tags (GTST) from insertional mutations generated in mouse embryonic stem (ES) cells. Funded by the Federal Ministry of Education and Research ( BMBF.
genetrap.helmholtz-muenchen.de
GGTC German Gene Trap Consortium
2910 new clones released. Clones are generated with enhanced gene trap vectors. 8247 new gene trap sequence tags released. Release of the new GGTC web interface. New release of GGTC gene trap library:. More than 35000 gene trap sequence tags analyzed by genomic PCR. The German Genetrap Consortium (GGTC). Generates a reference library of gene trap sequence tags (GTST) from insertional mutations generated in mouse embryonic stem (ES) cells. Funded by the Federal Ministry of Education and Research ( BMBF.
IGTC, International Gene Trap Consortium
Gene trapping is a high-throughput approach that is used to introduce insertional mutations across the genome in mouse embryonic stem (ES) cells. In addition to generating standard loss-of-function alleles, newer gene trap vectors offer a variety of post-insertional modification strategies for the generation of other experimental alleles. IGTC cell lines in database:. All news and events.
.: TIGEM - IRBM GeneTRAP Project :.
Several collections of mutated mouse embryonic stem cell lines are available. The objective of the joint IRBM-TIGEM TRAP-FLIP project is to demonstrate the feasibility of constructing a library of ES cell clones in which each expressed gene is tagged with cassette exchange sites. Our system is based on a novel types of gene trap vectors containing two heterospecific FRT sites, and on exchange vectors designed to exchange the trapped cassette with any gene of interest. Search by Clone ID(s).
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