tol2kit.blogspot.com
Tol2kit: Questions on the Tol2kit, 3/6/10-4/3/12
http://tol2kit.blogspot.com/2010/04/questions-on-tol2kit-3610-onwards.html
A multisite Gateway-based construction kit for Tol2 expression constructs. Welcome to the Tol2kit blog. This is a forum for discussing the Tol2kit. The Lawson lab's related system. Is a set of constructs that can be used with Invitrogen's multisite Gateway recombination-based cloning system to build expression constructs in a Tol2 transposon backbone (derived from constructs from the Kawakami lab. Tuesday, April 6, 2010. Questions on the Tol2kit, 3/6/10-4/3/12. Thanks for your time. Thanks for your time!
zdm6.imib.es
ZDM6 - Keynote and invited speakers
http://www.zdm6.imib.es/zdm6/keynoteSpeakers.jsf
Esta web utiliza cookies. Si continúas navegando consideramos que aceptas su uso. Más información. Keynote and invited speakers. Keynote and invited speakers. Keynote and invited speakers. University of Wisconsin-Madison, USA). Dana-Farber Cancer Institute, USA). University of Washington, USA). University of California at San Diego, USA). Leonard I. Zon. Harvard Medical School, USA). María L. Cayuela. University Hospital Virgen Arrixaca, Spain). The University of Auckland, New Zealand).
tol2kit.blogspot.com
Tol2kit: Questions on the Tol2kit, 4/26/08-6/8/09
http://tol2kit.blogspot.com/2008/04/questions-on-tol2kit-42607-onwards.html
A multisite Gateway-based construction kit for Tol2 expression constructs. Welcome to the Tol2kit blog. This is a forum for discussing the Tol2kit. The Lawson lab's related system. Is a set of constructs that can be used with Invitrogen's multisite Gateway recombination-based cloning system to build expression constructs in a Tol2 transposon backbone (derived from constructs from the Kawakami lab. Saturday, April 26, 2008. Questions on the Tol2kit, 4/26/08-6/8/09. May 6, 2008 at 9:17 AM. In terms of entr...
tol2kit.blogspot.com
Tol2kit: February 2014
http://tol2kit.blogspot.com/2014_02_01_archive.html
A multisite Gateway-based construction kit for Tol2 expression constructs. Welcome to the Tol2kit blog. This is a forum for discussing the Tol2kit. The Lawson lab's related system. Is a set of constructs that can be used with Invitrogen's multisite Gateway recombination-based cloning system to build expression constructs in a Tol2 transposon backbone (derived from constructs from the Kawakami lab. Saturday, February 22, 2014. Temporary Tol2kit wiki site. Thank you for your patience. The constructs in the...
tol2kit.genetics.utah.edu
Basic Gateway principles - Tol2kit
http://tol2kit.genetics.utah.edu/index.php/Basic_Gateway_principles
NB: The Tol2kit uses three-insert multisite Gateway cloning (att4-att1-att2-att3) from Invitrogen. It is not. Easily compatible with the newer "Gateway Pro" system, for which the outermost sites are att1-att2. Principles of Gateway recombination. Here's a diagram of a Gateway BP reaction, showing an example of how a "middle" clone (pME) is constructed using PCR with gene-specific primers to add att. Sites, then recombination with a donor vector. How we use this in the Tol2kit. Given the different clones ...
tol2kit.genetics.utah.edu
List of entry and destination vectors - Tol2kit
http://tol2kit.genetics.utah.edu/index.php/List_of_entry_and_destination_vectors
List of entry and destination vectors. Here is a list of the constructs in the Tol2kit. Click on any construct name (second column) to see more information. 12/18/07: Clone pages now include annotated sequence in Genbank format. Are insert sequences for entry clones, and 5' and 3' ends for destination clones, along with a description of how to assemble predicted sequences for expression clones. Constructs in the Tol2kit v1.2 (Nov 2007). 5' entry clones, attL4-R1 (kan resistant). SV40 late polyA signal.
tol2kit.genetics.utah.edu
Tol2Kit:General disclaimer - Tol2kit
http://tol2kit.genetics.utah.edu/index.php/Tol2Kit:General_disclaimer
There is currently no text in this page, you can search for this page title. In other pages or edit this page. Retrieved from " http:/ tol2kit.genetics.utah.edu/index.php/Tol2Kit:General disclaimer. Log in / create account.
tol2kit.genetics.utah.edu
Protocols - Tol2kit
http://tol2kit.genetics.utah.edu/index.php/Protocols
Here are optimized methods and tips from the Chien lab, adopted after trial and error, which work consistently in our hands. See the Invitrogen multisite Gateway manual. For all of the basic information necessary to understand and perform Gateway recombination reactions. See also the Gateway tips. On the Lawson lab website. Please report problems or questions on the Tol2kit blog. What you will need. PCR Amplification of DNA. Purification of PCR products. Factors Affecting Reaction Efficiency. Provides ne...
tol2kit.genetics.utah.edu
Att site sequences - Tol2kit
http://tol2kit.genetics.utah.edu/index.php/Att_site_sequences
12-09-07 Minor edits to fix a couple of sequences and clarify names (especially the attBXX primer sequences). Att site shared sequences. Here is a set of "shared" sequences- the core stretch that is shared between attB/P/L/R for att1-4. These are useful for stitching together entry and destination sequences to calculate the sequence of a final expression clone. Att1 shared TTTGTACAAAAAAG att2 shared CTTTCTTGTACAAAGT att3 shared CAACTTTATTATACA att4 shared CAACTTTGTATAGAAAAGTTG. List of att sites.