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Gene-Quantification.info - The reference in qPCR - Academic & Industrial Information Platform

Academic & industrial information platform for qPCR - The page describes and summarises all technical aspects involved in quantitative gene expression analysis using real-time qPCR & qRT-PCR.

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Gene-Quantification.info - The reference in qPCR - Academic & Industrial Information Platform | qpcrupdate.eu Reviews
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Academic & industrial information platform for qPCR - The page describes and summarises all technical aspects involved in quantitative gene expression analysis using real-time qPCR & qRT-PCR.
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4 application
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Gene-Quantification.info - The reference in qPCR - Academic & Industrial Information Platform | qpcrupdate.eu Reviews

https://qpcrupdate.eu

Academic & industrial information platform for qPCR - The page describes and summarises all technical aspects involved in quantitative gene expression analysis using real-time qPCR & qRT-PCR.

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1

Posterboard A on Gene-Quantification.info

http://www.qpcrupdate.eu/poster-a.html

Poster board A: Array verification. Most posters are in Adobe Acrobat Format ( PDF ). Using real-time PCR for cDNA Array verification. Birgit Kindermann, Hannelore Daniel, Michael W. Pfaffl and Frank Döring (2002). Einsatz der cDNA-Array-Technologie zur Identifizierung Zink-sensitiver Gene in-vitro. Dechema Statusseminar „Chiptechnologie: Transkriptom – Proteom – Metabolom“, S.79. Birgit Kindermann, Hannelore Daniel, Michael W. Pfaffl and Frank Döring (2002). Array Meeting: COST B20: Mammary development,...

2

Array and real-time PCR - BioInformatics

http://www.qpcrupdate.eu/array.html

Using real-time PCR for cDNA Array verification. Accuracy and calibration of commercial oligonucleotide and custom cDNA microarrays. Yuen T, Wurmbach E, Pfeffer RL, Ebersole BJ, Sealfon SC. Department of Neurology, Mount Sinai School of Medicine, New York, NY 10029, USA. Nucleic Acids Res. 2002 30(10): e48. Wurmbach E, Yuen T, Sealfon SC. Methods. 2003 31(4): 306-316. Department of Neurology, Mount Sinai School of Medicine, Box 1137, 1 Gustave L. Levy Place, New York, NY 10029, USA. Memorec Biotech GmbH,...

3

Normalisation of real-time RT-PCR reactions

http://www.qpcrupdate.eu/hkg.html

Reference Genes / Housekeeping Genes (1). In real-time PCR - page 2. In real-time PCR - page 3. In real-time PCR - page 4. Data normalisation in real-time RT-PCR is a further major step in gene quantification analysis ( Bustin 2002. Any variation in the normalizer will obscure real changes and produce artifactual changes. And cDNA sample loading variation ( Stahlberg 2003. Pfaffl and Hageleit 2001). Or Ribogreen RNA Quantification Kit. Alternatively the rRNA content has been proposed as an optimal an...

4

Gene Quantification & competitive RT-PCR

http://www.qpcrupdate.eu/competitive.html

Competitive RT-PCR vs. real-time RT-PCR. RT-PCR and real-time RT-PCR (all). Literature: classical block RT-PCR & competitive RT-PCR. Quantitative reverse transcription-polymerase chain reaction (RT-PCR): a. Comparison of primer-dropping, competitive, and real-time RT-PCRs. Flow Cytometric Quantification of Competitive Reverse Transcription-PCR Products. Quantification of Insulin-like Growth Factor-1 (IGF-1) mRNA: Development and validation of an. Internally standardised competitive RT-PCR. Calculated bas...

5

Gene Quantification & block RT-PCR

http://www.qpcrupdate.eu/block.html

Polymerase Chain Reaction (PCR). Reverse Transcription - Polymerase Chain Reaction (RT-PCR). The Nobel Prize in Chemistry 1993. Polymerase Chain Reaction -. The PCR method – a copying machine for DNA molecules. Http:/ nobelprize.org/nobel prizes/chemistry/laureates/1993/illpres/pcr.html. Roche Applied Science - PCR Application Manual 3rd Edition. PCR and RT-PCR are techniques so common in today's laboratories that it is easy to forget its. Any given cycle that falls within the reaction's exponential range.

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Gene-Quantification.info - The reference in qPCR - Academic & Industrial Information Platform

BioScience Events - Dr. Michael W. Pfaffl editor@gene.quantification.info. Main focus of the GENE QUANTIFICATION web page. Is, to describe and summarize all technical aspects. Involved in quantitative gene expression analysis using real-time (kinetic) PCR & RT-PCR. Eg detection chemistry, assay specificity, sensitivity, reproducibility, irobustness,. Intra- and inter-assay variations, kinetic PCR efficiency calculation, quantification strategies,. Main focus of the PHYSIOLOGY web page. Description of qua...

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Gene-Quantification.info - The reference in qPCR - Academic & Industrial Information Platform

BioScience Events - Dr. Michael W. Pfaffl editor@gene.quantification.info. Main focus of the GENE QUANTIFICATION web page. Is, to describe and summarize all technical aspects. Involved in quantitative gene expression analysis using real-time (kinetic) PCR & RT-PCR. Eg detection chemistry, assay specificity, sensitivity, reproducibility, irobustness,. Intra- and inter-assay variations, kinetic PCR efficiency calculation, quantification strategies,. Main focus of the PHYSIOLOGY web page. Description of qua...

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Gene-Quantification.info - The reference in qPCR - Academic & Industrial Information Platform

BioScience Events - Dr. Michael W. Pfaffl editor@gene.quantification.info. Main focus of the GENE QUANTIFICATION web page. Is, to describe and summarize all technical aspects. Involved in quantitative gene expression analysis using real-time (kinetic) PCR & RT-PCR. Eg detection chemistry, assay specificity, sensitivity, reproducibility, irobustness,. Intra- and inter-assay variations, kinetic PCR efficiency calculation, quantification strategies,. Main focus of the PHYSIOLOGY web page. Description of qua...

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BioScience Events - Dr. Michael W. Pfaffl editor@gene.quantification.info. Main focus of the GENE QUANTIFICATION web page. Is, to describe and summarize all technical aspects. Involved in quantitative gene expression analysis using real-time (kinetic) PCR & RT-PCR. Eg detection chemistry, assay specificity, sensitivity, reproducibility, irobustness,. Intra- and inter-assay variations, kinetic PCR efficiency calculation, quantification strategies,. Main focus of the PHYSIOLOGY web page. Description of qua...

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Gene-Quantification.info - The reference in qPCR - Academic & Industrial Information Platform

BioScience Events - Dr. Michael W. Pfaffl editor@gene.quantification.info. Main focus of the GENE QUANTIFICATION web page. Is, to describe and summarize all technical aspects. Involved in quantitative gene expression analysis using real-time (kinetic) PCR & RT-PCR. Eg detection chemistry, assay specificity, sensitivity, reproducibility, irobustness,. Intra- and inter-assay variations, kinetic PCR efficiency calculation, quantification strategies,. Main focus of the PHYSIOLOGY web page. Description of qua...

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