Academic & industrial information platform for qPCR - The page describes and summarises all technical aspects involved in quantitative gene expression analysis using real-time qPCR & qRT-PCR.
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Gene-Quantification.info - The reference in qPCR - Academic & Industrial Information Platform | qpcrupdate.org Reviews
https://qpcrupdate.org
Academic & industrial information platform for qPCR - The page describes and summarises all technical aspects involved in quantitative gene expression analysis using real-time qPCR & qRT-PCR.
qpcrupdate.org
Gene Quantification & real-time PCR dyes and chemistries
http://www.qpcrupdate.org/chemistry.html
Dyes and Fluorescence detection chemistry in qPCR. Short summary of detection formats. HRM - High Resolution Melt Dyes. Amplifluor Quantitative PCR Detection System. LUX (Light Upon eXtension) primers. The iCycler iQ Detection System. For multiplex real-time PCR Assays. A novel real-time quantitative PCR method using attached universal template probe. SYBR Green based Quantitative PCR. Flexible – SYBR Green JumpStart Taq ReadyMixes for SYBR based QPCR are formulated with MgCl2 or packaged with a se...
Gene Quantification & optimisation real-time / kinetic PCR/ RT-PCR reactions
http://www.qpcrupdate.org/optimization.html
Real-time PCR optimisation (1). Real-time PCR optimisation (2). Real-time PCR optimisation (3). Real-time PCR optimisation (4). Real-time PCR optimisation (5). Eleven golden rules of quantitative RT-PCR. The MIQE press review. The reproducibility of biomedical research: Sleepers awake! Stephen A. Bustin,. Biomolecular Detection and Quantification, Volume 2, December 2014, Pages 35–42. Current problems in quantitative real-time RT-PCR. T Nolan, RE. Hands and SA Bustin;. Total or mRNA,. Is exacerbated by s...
Gene Quantification & real-time PCR / kinetic RT-PCR
http://www.qpcrupdate.org/real-time.html
Real-time PCR and RT-PCR (1). Real-time PCR and RT-PCR (2). Real-time PCR and RT-PCR (3). QPCR - a technique enabling fast, quantitative and reliable results. Some of the limitations of end-point detection in (RT-) PCR have been assuaged in real-time PCR systems. Each of these systems employs either one of several general types of fluorescent probes for detection. Several different basic types of fluorescent probes are used for real-time PCR applications. QPCR, dPCR, NGS – A journey. Which are the longes...
Available real-time RT-PCR cycler and kinetic PCR machines
http://www.qpcrupdate.org/platform.html
Available real-time PCR cyclers (1). Available real-time PCR cyclers (2). Available real-time PCR cyclers (3). Available real-time PCR cyclers (4). A hopefully complete list of the commercially available real-time PCR systems are overviewed on this page and/or summarized below:. 7500 FAST real-time PCR system. Life Technologies™ - Applied Biosystems: ViiA. Life Technologies™ - Applied Biosystems: QuantStudio. 8482; real-time PCR system. Roche: LightCycler 2.0. Idaho Technology: RapidCycler 2. As the list...
BioInformatics & relative quantification using real timePCR
http://www.qpcrupdate.org/relative.html
Relative Quantification or Comparative Quantification in qPCR. Calculating the relative expression ratio. Relative quantification models WITHOUT efficiency correction. Relative quantification models WITH efficiency correction - using averaged mean PCR efficiency. Relative quantification models WITH efficiency correction - using single-run PCR efficiency. Go to efficiency correction page. Chapter 3: Relative Quantification. By Michael W. Pfaffl. In: Real-time PCR. (Editor: T. Dorak), p 63-82. Chini V, Fok...
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www.qPCR.asia & www.real-time-PCR.asia & www.Gene-Quantification.asia
WwwqPCR.asia and www.real-time-PCR.asia and www.Gene-Quantification.asia - Gene-Quantification.asia - The reference in qPCR - Academic and Industrial Information Platform - qpcrupdate.asia.
Gene-Quantification.info - The reference in qPCR - Academic & Industrial Information Platform
BioScience Events - Dr. Michael W. Pfaffl editor@gene.quantification.info. Main focus of the GENE QUANTIFICATION web page. Is, to describe and summarize all technical aspects. Involved in quantitative gene expression analysis using real-time (kinetic) PCR & RT-PCR. Eg detection chemistry, assay specificity, sensitivity, reproducibility, irobustness,. Intra- and inter-assay variations, kinetic PCR efficiency calculation, quantification strategies,. Main focus of the PHYSIOLOGY web page. Description of qua...
Gene-Quantification.info - The reference in qPCR - Academic & Industrial Information Platform
BioScience Events - Dr. Michael W. Pfaffl editor@gene.quantification.info. Main focus of the GENE QUANTIFICATION web page. Is, to describe and summarize all technical aspects. Involved in quantitative gene expression analysis using real-time (kinetic) PCR & RT-PCR. Eg detection chemistry, assay specificity, sensitivity, reproducibility, irobustness,. Intra- and inter-assay variations, kinetic PCR efficiency calculation, quantification strategies,. Main focus of the PHYSIOLOGY web page. Description of qua...
Gene-Quantification.info - The reference in qPCR - Academic & Industrial Information Platform
BioScience Events - Dr. Michael W. Pfaffl editor@gene.quantification.info. Main focus of the GENE QUANTIFICATION web page. Is, to describe and summarize all technical aspects. Involved in quantitative gene expression analysis using real-time (kinetic) PCR & RT-PCR. Eg detection chemistry, assay specificity, sensitivity, reproducibility, irobustness,. Intra- and inter-assay variations, kinetic PCR efficiency calculation, quantification strategies,. Main focus of the PHYSIOLOGY web page. Description of qua...
Gene-Quantification.info - The reference in qPCR - Academic & Industrial Information Platform
BioScience Events - Dr. Michael W. Pfaffl editor@gene.quantification.info. Main focus of the GENE QUANTIFICATION web page. Is, to describe and summarize all technical aspects. Involved in quantitative gene expression analysis using real-time (kinetic) PCR & RT-PCR. Eg detection chemistry, assay specificity, sensitivity, reproducibility, irobustness,. Intra- and inter-assay variations, kinetic PCR efficiency calculation, quantification strategies,. Main focus of the PHYSIOLOGY web page. Description of qua...
Gene-Quantification.info - The reference in qPCR - Academic & Industrial Information Platform
BioScience Events - Dr. Michael W. Pfaffl editor@gene.quantification.info. Main focus of the GENE QUANTIFICATION web page. Is, to describe and summarize all technical aspects. Involved in quantitative gene expression analysis using real-time (kinetic) PCR & RT-PCR. Eg detection chemistry, assay specificity, sensitivity, reproducibility, irobustness,. Intra- and inter-assay variations, kinetic PCR efficiency calculation, quantification strategies,. Main focus of the PHYSIOLOGY web page. Description of qua...
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