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SIP AY09/10

Tuesday, November 10, 2009. Sorry for the very late posting. This would be the final post of the whole 20 weeks! HahaI would like to talk about Microtomy as it the the last area that i'm posted to. The principle of microtomy is to sectioned tissue specimens into thin sections so that they can viewed and diagnose under microscope. Below is a picture of the rotary microtome used for microtomy. Alcohol Floatation bath 1. Microscopic glass slides 1. Disposable microtome blade 1. Warm floatation bath 1. In ou...

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SIP AY09/10 | sesamechicken90.blogspot.com Reviews
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Tuesday, November 10, 2009. Sorry for the very late posting. This would be the final post of the whole 20 weeks! HahaI would like to talk about Microtomy as it the the last area that i'm posted to. The principle of microtomy is to sectioned tissue specimens into thin sections so that they can viewed and diagnose under microscope. Below is a picture of the rotary microtome used for microtomy. Alcohol Floatation bath 1. Microscopic glass slides 1. Disposable microtome blade 1. Warm floatation bath 1. In ou...
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SIP AY09/10 | sesamechicken90.blogspot.com Reviews

https://sesamechicken90.blogspot.com

Tuesday, November 10, 2009. Sorry for the very late posting. This would be the final post of the whole 20 weeks! HahaI would like to talk about Microtomy as it the the last area that i'm posted to. The principle of microtomy is to sectioned tissue specimens into thin sections so that they can viewed and diagnose under microscope. Below is a picture of the rotary microtome used for microtomy. Alcohol Floatation bath 1. Microscopic glass slides 1. Disposable microtome blade 1. Warm floatation bath 1. In ou...

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sesamechicken90.blogspot.com sesamechicken90.blogspot.com
1

SIP AY09/10: August 2009

http://sesamechicken90.blogspot.com/2009_08_01_archive.html

Saturday, August 29, 2009. Normal urothelial and cervical cells are present. Below is a picture of normal urine papanicolaou stain. However, if patient has any abnormalities such as infections, the papanicolaou stains will be different. Below are pictures on various types of infections. Patient might also be having urothelial carcinoma. Below is the picture of this example. Scums need to be removed every morning to ensure the quality of the slides are maintained. Friday, August 21, 2009. 5) Wash in water.

2

SIP AY09/10: October 2009

http://sesamechicken90.blogspot.com/2009_10_01_archive.html

Saturday, October 31, 2009. Hi people, this is my last post for SIP. Hope you guys are doing fine for your project. :) I have been posted to the trimming room for this month and will be talking about processing for this post. Principle: It is to remove the water contain inside the tissues so that wax can infiltrate as wax is not miscible with water. (Ethanol is used in this case). Principle: It is to replace the clearing agent with embedding medium (Paraffin wax is used). Friday, October 23, 2009. 4 Herp...

3

SIP AY09/10: September 2009

http://sesamechicken90.blogspot.com/2009_09_01_archive.html

Sunday, September 27, 2009. Hi everyone, this is my third post. In this post, i am going to talk about processing of the gynaecology specimens in cytology lab. Basically, the gynae specimens come in two forms, Thin Prep vials or conventional smears. The thin prep vials contain preservcyt solution which is basically a methanol-based medium. The purpose is to preserve the cells to allow proper evaluation under microscope. 1)Specimen is collected with a spatula or a cytobrush. 3)The stain used is papanicola...

4

SIP AY09/10: November 2009

http://sesamechicken90.blogspot.com/2009_11_01_archive.html

Tuesday, November 10, 2009. Sorry for the very late posting. This would be the final post of the whole 20 weeks! HahaI would like to talk about Microtomy as it the the last area that i'm posted to. The principle of microtomy is to sectioned tissue specimens into thin sections so that they can viewed and diagnose under microscope. Below is a picture of the rotary microtome used for microtomy. Alcohol Floatation bath 1. Microscopic glass slides 1. Disposable microtome blade 1. Warm floatation bath 1. In ou...

5

SIP AY09/10: HbA1c test

http://sesamechicken90.blogspot.com/2009/10/hey-subject-title-clinical-chemistry.html

Sunday, October 11, 2009. Haemoglobin A1c (HbA1c) Test using Bio-Rad D-10. Note: I will just be focusing on the main procedure of running the samples. I will talk about the Calibration next week. 2D-10- Haemoglobin A1cRecorder Pack. A) Elution Buffer 1. B) Elution Buffer 2. G) Whole Blood Primer. 3Sample Vial Adapter, 10 x 1.5mL. 4Lymphochek Diabetes Bi-level Control, 6 x 0.5 mL. 5Pipettes, 5mL, 0.5mL, 1mL, 7mL. 1Start up the machine if it is in “Sleep” mode. 3Check the amount of blood present. 7 The ove...

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med-scientist-in-action.blogspot.com med-scientist-in-action.blogspot.com

MedScientist: Immunochemistry- G6PD Quantitative Screening Test

http://med-scientist-in-action.blogspot.com/2009/08/immunochemistry-g6pd-quantitative.html

Today's science fiction is tomorrow's science fact. Monday, August 24, 2009. Immunochemistry- G6PD Quantitative Screening Test. Its my turn to post once again, woo! Today I’m gonna share with you guys, the Glucose-6-phosphate dehydrogenase (G6PD) screening test (Quantitative). By the OSMMR-D-D assay, which is a new machine currently under evaluation by the Immunochemistry lab. (which is the lab I’m permanently attached to.). End of story, good bye! Joanna Yeo, woo! MedScientists of Grp 6. Hope you are we...

med-scientist-in-action.blogspot.com med-scientist-in-action.blogspot.com

MedScientist: June 2009

http://med-scientist-in-action.blogspot.com/2009_06_01_archive.html

Today's science fiction is tomorrow's science fact. Tuesday, June 30, 2009. My 1st week of SIP in 2 Departments! This posting is for this week). My first week of attachment was filled with so much fun and laughter. I was posted to 2 departments. For a start,. Contents of my post:. 1) Briefly describing the daily routine work in Histology Department. 2) Operation of the Tissue Processor. 3) Principles of the common stains. 1) Organs and other specimens are received. 8) Slides were stained. Http:/ www....

med-scientist-in-action.blogspot.com med-scientist-in-action.blogspot.com

MedScientist: Transformation of chemically ultra-competent E.coli with plasmid DNA

http://med-scientist-in-action.blogspot.com/2009/09/transformation-of-chemically-ultra.html

Today's science fiction is tomorrow's science fact. Monday, September 21, 2009. Transformation of chemically ultra-competent E.coli with plasmid DNA. Posted by Vo Thu Hong Anh [Jess] 0705364H. To generate a large amount of plasmid DNA containing a desired gene, with the aid of chemically ultra-competent E.coli. Chemically ultra-competent E.coli. Cells will also be heated at 42. Place the paper disc containing plasmid DNA (commercially made and shipped) into a 1.5mL-centrifuge tube. On ice to thaw. After ...

med-scientist-in-action.blogspot.com med-scientist-in-action.blogspot.com

MedScientist: August 2009

http://med-scientist-in-action.blogspot.com/2009_08_01_archive.html

Today's science fiction is tomorrow's science fact. Monday, August 24, 2009. Immunochemistry- G6PD Quantitative Screening Test. Its my turn to post once again, woo! Today I’m gonna share with you guys, the Glucose-6-phosphate dehydrogenase (G6PD) screening test (Quantitative). By the OSMMR-D-D assay, which is a new machine currently under evaluation by the Immunochemistry lab. (which is the lab I’m permanently attached to.). End of story, good bye! Joanna Yeo, woo! MedScientists of Grp 6. The test in my ...

med-scientist-in-action.blogspot.com med-scientist-in-action.blogspot.com

MedScientist: Bone Marrow Aspiration!

http://med-scientist-in-action.blogspot.com/2009/10/bone-marrow-aspiration.html

Today's science fiction is tomorrow's science fact. Tuesday, October 6, 2009. I will discuss with all of you about Bone Marrow Aspiration (BMA) and the respective tests done. First, what is BMA? It is the removal of a small amount of bone marrow, which is the soft tissue in liquid form inside the bones (place where blood cells formed; hollow part of most bones) for examination. Why do we need to do BMA? Where is BMA obtain from? How is BMA obtain? Traplok Bone Marrow Biopsy Needle (blue T-shape needle is...

bordetellas.blogspot.com bordetellas.blogspot.com

Bordetellas Logbook:]: July 2009

http://bordetellas.blogspot.com/2009_07_01_archive.html

Group Members: Eriko Wong; Nurul Natasha; Nurul Nyzah; Ong Wendy; Siti Shahimah. Tuesday, July 28, 2009. Hey Siti Shahimah is here again. :). Okay, so I have learnt about TDx/IMX. Basically, TDx/IMX are the name of analyzers that help to detect therapeutic dosage of drugs so as to ensure appropriate therapy for patients. TDx analyzes drugs such as cyclosporine, methotrexate and everolimus. Cyclosporine and everolimus are immunosuppresant drugs whereas methotrexate is an antineoplastic drug. 150 uL of pat...

bordetellas.blogspot.com bordetellas.blogspot.com

Bordetellas Logbook:]: September 2009

http://bordetellas.blogspot.com/2009_09_01_archive.html

Group Members: Eriko Wong; Nurul Natasha; Nurul Nyzah; Ong Wendy; Siti Shahimah. Saturday, September 26, 2009. For hematology section, tests such as full blood count (FBC), erythrocyte sedimentation rate ESR), peripheral blood films (PBF), and retic count are done. there are also malaria tests and dengue panel. Sunday, September 13, 2009. General theory of using an organic solvent to precipitate out proteins in plasma:. Organic solvent reduces water activity. This is done by:. 5 Vortex for 2 minutes.

bordetellas.blogspot.com bordetellas.blogspot.com

Bordetellas Logbook:]: blood collection

http://bordetellas.blogspot.com/2009/10/blood-collection.html

Group Members: Eriko Wong; Nurul Natasha; Nurul Nyzah; Ong Wendy; Siti Shahimah. Sunday, October 11, 2009. In this post, I am going to write about a tutorial given by one of our colleague. All of us have to attend this tutorials unless we are busy at our work stations. These tutorials are given at least once every 2 weeks. Topic for today: blood collection. Times to provide thorough mixing of the additives. Also, it is important to note that shaking of blood tubes may cause hemolysis of the blood. They c...

bordetellas.blogspot.com bordetellas.blogspot.com

Bordetellas Logbook:]: ways to protect the column from damaging

http://bordetellas.blogspot.com/2009/11/ways-to-protect-column-from-damaging.html

Group Members: Eriko Wong; Nurul Natasha; Nurul Nyzah; Ong Wendy; Siti Shahimah. Thursday, November 12, 2009. Ways to protect the column from damaging. Sorry for the super late post! Since the column of the HPLC is very expensive, we can have to do things to maximize the length of its life:). Here are the things to do:. During shutdown, always flush the column with ultrapure water to remove all the salts present from the buffer. If this is not done, salts will precipitate and cause clogging of column.

bordetellas.blogspot.com bordetellas.blogspot.com

Bordetellas Logbook:]: Immunofixation Electrophoresis

http://bordetellas.blogspot.com/2009/10/immunofixation-electrophoresis.html

Group Members: Eriko Wong; Nurul Natasha; Nurul Nyzah; Ong Wendy; Siti Shahimah. Friday, October 16, 2009. 1 Proteins in the samples (urine and serum) are separated by electrophoresis on agarose gel. 2 Immunofixation of electrophoresed proteins includes, the monospecific antisera diffuse into. Agarose gel and bind to the antigens (proteins), if present. Thus, resulting in the precipitation. 3 The unprecipitated antigens are removed from the gel by blotting and washing. Of the fractions is dark in colour.

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SIP AY09/10

Tuesday, November 10, 2009. Sorry for the very late posting. This would be the final post of the whole 20 weeks! HahaI would like to talk about Microtomy as it the the last area that i'm posted to. The principle of microtomy is to sectioned tissue specimens into thin sections so that they can viewed and diagnose under microscope. Below is a picture of the rotary microtome used for microtomy. Alcohol Floatation bath 1. Microscopic glass slides 1. Disposable microtome blade 1. Warm floatation bath 1. In ou...

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