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Serving the trypanosome research. The top left panel shows original drawings by Surgeon-Captain David Bruce, of the British Army Medical Service, who identified Trypanosoma brucei as the cause of the 'tsetse fly disease' in African cattle in 1895. The human disease is called Sleeping Sickness. Infection leads rapidly to coma and death. The left panel shows a color-enhanced scanning electron micrograph of Trypanosoma brucei among blood cells. Michael Duszenko, University of Tübingen, Germany. Clear your c...

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TrypsRU Home | tryps.rockefeller.edu Reviews
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Serving the trypanosome research. The top left panel shows original drawings by Surgeon-Captain David Bruce, of the British Army Medical Service, who identified Trypanosoma brucei as the cause of the 'tsetse fly disease' in African cattle in 1895. The human disease is called Sleeping Sickness. Infection leads rapidly to coma and death. The left panel shows a color-enhanced scanning electron micrograph of Trypanosoma brucei among blood cells. Michael Duszenko, University of Tübingen, Germany. Clear your c...
<META>
KEYWORDS
1 TRYPANOSOMIASIS
2 AFRICAN SLEEPING SICKNESS
3 NAGANA
4 TSETSE
5 GLOSSINA
6 CHAGAS DISEASE
7 TRYPANOSOME
8 KINETOPLASTIDA
9 PROTOZOA
10 TRYPANOSOMA BRUCEI
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trypsrockefeller edu,weather forecast,what are trypanosomes,why study trypanosomes,african sleeping sickness,antigenic variation,research resources,general protocols,cell lines,culture methods,introduction,detailed review,media composition,genome,analyses
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TrypsRU Home | tryps.rockefeller.edu Reviews

https://tryps.rockefeller.edu

Serving the trypanosome research. The top left panel shows original drawings by Surgeon-Captain David Bruce, of the British Army Medical Service, who identified Trypanosoma brucei as the cause of the 'tsetse fly disease' in African cattle in 1895. The human disease is called Sleeping Sickness. Infection leads rapidly to coma and death. The left panel shows a color-enhanced scanning electron micrograph of Trypanosoma brucei among blood cells. Michael Duszenko, University of Tübingen, Germany. Clear your c...

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tryps.rockefeller.edu tryps.rockefeller.edu
1

Cre-lox Systems

http://tryps.rockefeller.edu/trypsru2_cre-lox.html

Cre-lox (floxed) systems for gene deletion ( click here. For non-floxed and earlier floxed tagging vectors). The baseline reference for this system is CRE recombinase-based positive-negative selection systems for genetic manipulation in Trypanosoma brucei. 2008) Scahill MD, Pastar I and Cross GAM. Molecular and Biochemical Parasitology 157:73-82. DOI. It is suggested that you read at least parts of this paper, especially the discussion! 2013) Kim, H-S, Li Z, Boothroyd, C and Cross, GAM, Molecular and Boc...

2

publications pre-1980

http://tryps.rockefeller.edu/trypsru2_publications3.html

Publications prior to 1980 (most recent first). 1979: Cross-reacting determinants in the C-terminal region of trypanosome variant surface antigens. Cross GAM. Nature 277:310-2. 1979: Immunochemical aspects of antigenic variation in trypanosomes. The Third Fleming Lecture. Cross GAM. J Gen Microbiol 113:1-11. 1979: Selective cleavage of variant surface glycoproteins from Trypanosoma brucei. Johnson JG and Cross GAM. Biochem J 178:689-97. 1978: Antigenic variation in trypanosomes. Taylor DW and Cross GAM&#...

3

Sleeping Sickness: about the disease

http://tryps.rockefeller.edu/trypsru2_sleeping_sickness.html

Sleeping sickness (human African trypanosomiasis). This page is a work in progress. If you will be travelling to Africa and need more information about sleeping sickness and how to avoid and treat it, you can find this at the Centers for Disease Control and Prevention. Or the World Health Organization. Click on the left panel to return to our home page. Good News (July 2010) on Human African trypanosomiasis from WHO:. Number of new cases drops to lowest level in 50 years.

4

media compsitions tabulated

http://tryps.rockefeller.edu/trypsru2_culture_media_compositions.html

Culture media composition notes. Back to culture introduction. This summary of compositions has been prepare to facilitate comparison between some of the (sometimes quite bizarre) culture media developed for Trypanosoma brucei. Although it has been quite painstaking to prepare this, it could conceivably contain some errors. Our JRH custom Cunningham's formulation has removed some very probably unnecessary components. The manufacturer may also have changed some salts from H. Is it a primitive buffer?

5

TrypsRU Home

http://tryps.rockefeller.edu/default.html

Serving the trypanosome research. The top left panel shows original drawings by Surgeon-Captain David Bruce, of the British Army Medical Service, who identified Trypanosoma brucei as the cause of the 'tsetse fly disease' in African cattle in 1895. The human disease is called Sleeping Sickness. Infection leads rapidly to coma and death. The left panel shows a color-enhanced scanning electron micrograph of Trypanosoma brucei among blood cells. Michael Duszenko, University of Tübingen, Germany. Clear your c...

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wicksteadlab.co.uk wicksteadlab.co.uk

Wickstead Lab

http://wicksteadlab.co.uk/links.shtml

Some useful trypanosome links. This list is in no way supposed to be exhaustive, just some of the online resources that we go to most often. My apologies if you have been left out! Includes more trypanosome expression and tagging constructs. Lots of resources, protocols and information on growing trypanosomes, etc. More constructs for trypanosomes. P2T7 derivatives and TrypanoFAN links. David Horn also maintains a great list of links to other trypanosome research labs. The T brucei sequencing project.

wicksteadlab.co.uk wicksteadlab.co.uk

Wickstead Lab

http://wicksteadlab.co.uk/vectors.shtml

Below are maps and sequences for some of the plasmids for stable transformation of Trypanosoma brucei. Available from the Wickstead lab or collaborators. I've finally found an Open Source plasmid editor program that is, in my opinion, a reasonable substitute for GCK. M. Wayne Davis's ApE: A Plasmid Editor. Still presently included on this site are annotated Gene Construction Kit. I've written a small perl script that will take annotated ApE files and make a pretty SVG files from them (avaiable here.

catarinagadelha.com catarinagadelha.com

Catarina Gadelha webpages

http://www.catarinagadelha.com/links.shtml

Some useful online resources. Plenty of protocols, meeting information, resources etc. List of links to other trypanosome research labs, and more. Trypanosome vectors, tools, and other stuff. The WHO Special Programme for Research and Training in Tropical Diseases. Kinetoplastid genome resources that include integration of functional data. Part of the EuPathDB. Curated pathogen genome resourses from the Wellcome Trust Sanger Centre. At the Wellcome Trust Sanger Centre. At the Wellcome Trust Sanger Centre.

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tryps.rockefeller.edu tryps.rockefeller.edu

TrypsRU Home

Serving the trypanosome research. The top left panel shows original drawings by Surgeon-Captain David Bruce, of the British Army Medical Service, who identified Trypanosoma brucei as the cause of the 'tsetse fly disease' in African cattle in 1895. The human disease is called Sleeping Sickness. Infection leads rapidly to coma and death. The left panel shows a color-enhanced scanning electron micrograph of Trypanosoma brucei among blood cells. Michael Duszenko, University of Tübingen, Germany. Clear your c...

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The Trypshop

Wednesday, 12 August 2015. The Trypshop Show 13 "80's Psych Revival Part1" 12th August 2015. A show focusing on the 80's Psychedelic revivals that occured in the UK and USA. Includes a healthy selection from the Paisley Underground bands. Hosted as always by Mark Vidler. Tracks played on The Trypshop Show 13:. The Soft Boys - Underwater Moonlight (1980). Nick Nicely - 49 Cigars (1981). Mood Six - Just Like A Dream (1981). A Splash Of Colour). The Fleshtones - Shadow Line (1981). Days Of Wine And Roses).

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The Trypshop

The Trypshop show #13 80's Psych Revival Part1 (first aired 12th August 2015). Right click/save target as). Tunes played on Trypshop show #13:. The Soft Boys - Underwater Moonlight (1980). Nick Nicely - 49 Cigars (1981). Mood Six - Just Like A Dream (1981). A Splash Of Colour). The Fleshtones - Shadow Line (1981). Plasticland - Color Appreciation (1981). The Television Personalities - I Know Where Syd Barrett Lives (1981). The Times - Red With Purple Flashes (1981). Here Are The Chesterfield Kings).