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The Horton Lab

Wednesday, April 10, 2013. Transgenic mice - research update. As mentioned earlier, we have been working on identifying gene targets for soluble FGFR3 cleaved sICD fragment using ChIP-sequencing. We have made considerable progress in optimizing the protocol for this experiment and have also initiated our first ChIP-sequencing experiment. We are beginning to prepare for additional ChIP-sequencing experiments based on the results from our preliminary investigation. Jyoti Narayana, PhD. Jyoti Narayana, PhD.

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Wednesday, April 10, 2013. Transgenic mice - research update. As mentioned earlier, we have been working on identifying gene targets for soluble FGFR3 cleaved sICD fragment using ChIP-sequencing. We have made considerable progress in optimizing the protocol for this experiment and have also initiated our first ChIP-sequencing experiment. We are beginning to prepare for additional ChIP-sequencing experiments based on the results from our preliminary investigation. Jyoti Narayana, PhD. Jyoti Narayana, PhD.

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The Horton Lab | whortonlab.blogspot.com Reviews

https://whortonlab.blogspot.com

Wednesday, April 10, 2013. Transgenic mice - research update. As mentioned earlier, we have been working on identifying gene targets for soluble FGFR3 cleaved sICD fragment using ChIP-sequencing. We have made considerable progress in optimizing the protocol for this experiment and have also initiated our first ChIP-sequencing experiment. We are beginning to prepare for additional ChIP-sequencing experiments based on the results from our preliminary investigation. Jyoti Narayana, PhD. Jyoti Narayana, PhD.

INTERNAL PAGES

whortonlab.blogspot.com

1

The Horton Lab: Regulating FGFR3 degradation

http://www.whortonlab.blogspot.com/2012/09/regulating-fgfr3-degradation.html

Thursday, September 27, 2012. Down regulation of receptor signaling often involves internalization of the receptor followed by its degradation. Can take place through one or more pathways as shown in the figure below. Jyoti Narayana, PhD. Subscribe to: Post Comments (Atom). Horton Lab - Home. Jyoti Narayana, PhD. View my complete profile. Simple template. Powered by Blogger.

2

The Horton Lab: SELEX to identify gene targets

http://www.whortonlab.blogspot.com/2012/06/selex-to-identify-gene-targets.html

Friday, June 22, 2012. SELEX to identify gene targets. We have been looking at ways to identify genes that are regulated by FGFR3, specifically in the growth plate. To do so, we are working on a modified version of a technique called as SELEX ( S. Roulet, Emmanuelle, Stéphane Busso, Anamaria A Camargo, Andrew J G Simpson, Nicolas Mermod, and Philipp Bucher. “High-throughput SELEX SAGE Method for Quantitative Modeling of Transcription-factor Binding Sites.” Nature Biotechnology. Jyoti Narayana, PhD.

3

The Horton Lab: June 2012

http://www.whortonlab.blogspot.com/2012_06_01_archive.html

Friday, June 22, 2012. SELEX to identify gene targets. We have been looking at ways to identify genes that are regulated by FGFR3, specifically in the growth plate. To do so, we are working on a modified version of a technique called as SELEX ( S. Roulet, Emmanuelle, Stéphane Busso, Anamaria A Camargo, Andrew J G Simpson, Nicolas Mermod, and Philipp Bucher. “High-throughput SELEX SAGE Method for Quantitative Modeling of Transcription-factor Binding Sites.” Nature Biotechnology. Jyoti Narayana, PhD.

4

The Horton Lab: Expanding our current hypothesis

http://www.whortonlab.blogspot.com/2012/07/expanding-our-current-hypothesis.html

Monday, July 16, 2012. Expanding our current hypothesis. That brings us to our next question. Does sICD interact with other proteins? We are in the process of addressing some of these ideas. We will take a look at the techniques and experiments used to answer these questions. Jyoti Narayana, PhD. Subscribe to: Post Comments (Atom). Horton Lab - Home. Expanding our current hypothesis. Jyoti Narayana, PhD. View my complete profile. Simple template. Powered by Blogger.

5

The Horton Lab: Use of micromass cultures to study how sICD effects cell fate.

http://www.whortonlab.blogspot.com/2012/05/use-of-micromass-cultures-to-study-how.html

Monday, May 7, 2012. Use of micromass cultures to study how sICD effects cell fate. Bone, cartilage, and fat muscles are all formed from a common pool of embryonic mesoderm cells. The type of tissue (bone, fat or cartilage) that develops depends on specific stimulation that is produced through cellular signals. Signals involved in bone formation (osteogenesis), cartilage (chondrogenesis) and fat muscle (adipogenesis) are not well known. Shown in the diagram below, we plan to use. Jyoti Narayana, PhD.

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whortonlab2.blogspot.com

The Horton Lab: June 2013

http://whortonlab2.blogspot.com/2013_06_01_archive.html

Thursday, June 6, 2013. Subscribe to: Posts (Atom). View my complete profile. Simple template. Powered by Blogger.

whortonlab2.blogspot.com

The Horton Lab: Developing biomarkers to accurately assess bone growth

http://whortonlab2.blogspot.com/2013/04/developing-biomarkers-to-accurately.html

Wednesday, April 10, 2013. Developing biomarkers to accurately assess bone growth. As apparent from recent scientific publications and as discussed in blogs elsewhere in Growing Stronger, the prospects for growth stimulating therapy in achondroplasia are advancing at a fast pace. Clinical trials for the BioMarin analog of CNP are underway and other treatment strategies are being investigated in mouse models of achondroplasia, so-called preclinical studies. So the longstanding dream of normalizing...

whortonlab2.blogspot.com

The Horton Lab: April 2013

http://whortonlab2.blogspot.com/2013_04_01_archive.html

Wednesday, April 10, 2013. Developing biomarkers to accurately assess bone growth. As apparent from recent scientific publications and as discussed in blogs elsewhere in Growing Stronger, the prospects for growth stimulating therapy in achondroplasia are advancing at a fast pace. Clinical trials for the BioMarin analog of CNP are underway and other treatment strategies are being investigated in mouse models of achondroplasia, so-called preclinical studies. So the longstanding dream of normalizing...

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The Horton Lab

Wednesday, April 10, 2013. Transgenic mice - research update. As mentioned earlier, we have been working on identifying gene targets for soluble FGFR3 cleaved sICD fragment using ChIP-sequencing. We have made considerable progress in optimizing the protocol for this experiment and have also initiated our first ChIP-sequencing experiment. We are beginning to prepare for additional ChIP-sequencing experiments based on the results from our preliminary investigation. Jyoti Narayana, PhD. Jyoti Narayana, PhD.

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Thursday, June 6, 2013. Wednesday, April 10, 2013. Developing biomarkers to accurately assess bone growth. As apparent from recent scientific publications and as discussed in blogs elsewhere in Growing Stronger, the prospects for growth stimulating therapy in achondroplasia are advancing at a fast pace. Clinical trials for the BioMarin analog of CNP are underway and other treatment strategies are being investigated in mouse models of achondroplasia, so-called preclinical studies. So the longstand...

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