whortonlab.blogspot.com
The Horton Lab: March 2012
http://whortonlab.blogspot.com/2012_03_01_archive.html
Tuesday, March 27, 2012. Transgenic mice for FGFR3 signaling in vivo – Part II. Jyoti Narayana, PhD. Subscribe to: Posts (Atom). Horton Lab - Home. Transgenic mice for FGFR3 signaling in vivo – Part. Jyoti Narayana, PhD. View my complete profile. Simple template. Powered by Blogger.
whortonlab.blogspot.com
The Horton Lab: Regulating FGFR3 degradation
http://whortonlab.blogspot.com/2012/09/regulating-fgfr3-degradation.html
Thursday, September 27, 2012. Down regulation of receptor signaling often involves internalization of the receptor followed by its degradation. Can take place through one or more pathways as shown in the figure below. Jyoti Narayana, PhD. Subscribe to: Post Comments (Atom). Horton Lab - Home. Jyoti Narayana, PhD. View my complete profile. Simple template. Powered by Blogger.
whortonlab.blogspot.com
The Horton Lab: SELEX to identify gene targets
http://whortonlab.blogspot.com/2012/06/selex-to-identify-gene-targets.html
Friday, June 22, 2012. SELEX to identify gene targets. We have been looking at ways to identify genes that are regulated by FGFR3, specifically in the growth plate. To do so, we are working on a modified version of a technique called as SELEX ( S. Roulet, Emmanuelle, Stéphane Busso, Anamaria A Camargo, Andrew J G Simpson, Nicolas Mermod, and Philipp Bucher. “High-throughput SELEX SAGE Method for Quantitative Modeling of Transcription-factor Binding Sites.” Nature Biotechnology. Jyoti Narayana, PhD.
whortonlab.blogspot.com
The Horton Lab: Use of micromass cultures to study how sICD effects cell fate.
http://whortonlab.blogspot.com/2012/05/use-of-micromass-cultures-to-study-how.html
Monday, May 7, 2012. Use of micromass cultures to study how sICD effects cell fate. Bone, cartilage, and fat muscles are all formed from a common pool of embryonic mesoderm cells. The type of tissue (bone, fat or cartilage) that develops depends on specific stimulation that is produced through cellular signals. Signals involved in bone formation (osteogenesis), cartilage (chondrogenesis) and fat muscle (adipogenesis) are not well known. Shown in the diagram below, we plan to use. Jyoti Narayana, PhD.
whortonlab.blogspot.com
The Horton Lab: April 2013
http://whortonlab.blogspot.com/2013_04_01_archive.html
Wednesday, April 10, 2013. Transgenic mice - research update. As mentioned earlier, we have been working on identifying gene targets for soluble FGFR3 cleaved sICD fragment using ChIP-sequencing. We have made considerable progress in optimizing the protocol for this experiment and have also initiated our first ChIP-sequencing experiment. We are beginning to prepare for additional ChIP-sequencing experiments based on the results from our preliminary investigation. Jyoti Narayana, PhD. Horton Lab - Home.
whortonlab.blogspot.com
The Horton Lab: May 2012
http://whortonlab.blogspot.com/2012_05_01_archive.html
Monday, May 7, 2012. Use of micromass cultures to study how sICD effects cell fate. Bone, cartilage, and fat muscles are all formed from a common pool of embryonic mesoderm cells. The type of tissue (bone, fat or cartilage) that develops depends on specific stimulation that is produced through cellular signals. Signals involved in bone formation (osteogenesis), cartilage (chondrogenesis) and fat muscle (adipogenesis) are not well known. Shown in the diagram below, we plan to use. Jyoti Narayana, PhD.
whortonlab.blogspot.com
The Horton Lab: Expanding our current hypothesis
http://whortonlab.blogspot.com/2012/07/expanding-our-current-hypothesis.html
Monday, July 16, 2012. Expanding our current hypothesis. That brings us to our next question. Does sICD interact with other proteins? We are in the process of addressing some of these ideas. We will take a look at the techniques and experiments used to answer these questions. Jyoti Narayana, PhD. Subscribe to: Post Comments (Atom). Horton Lab - Home. Expanding our current hypothesis. Jyoti Narayana, PhD. View my complete profile. Simple template. Powered by Blogger.
whortonlab.blogspot.com
The Horton Lab: June 2012
http://whortonlab.blogspot.com/2012_06_01_archive.html
Friday, June 22, 2012. SELEX to identify gene targets. We have been looking at ways to identify genes that are regulated by FGFR3, specifically in the growth plate. To do so, we are working on a modified version of a technique called as SELEX ( S. Roulet, Emmanuelle, Stéphane Busso, Anamaria A Camargo, Andrew J G Simpson, Nicolas Mermod, and Philipp Bucher. “High-throughput SELEX SAGE Method for Quantitative Modeling of Transcription-factor Binding Sites.” Nature Biotechnology. Jyoti Narayana, PhD.
whortonlab.blogspot.com
The Horton Lab: January 2013
http://whortonlab.blogspot.com/2013_01_01_archive.html
Wednesday, January 30, 2013. We continue to work on ChIP-sequencing. Considering this will be the first time we will be performing a genome wide search for FGFR3 target genes, we have to do a lot of troubleshooting and run preliminary experiments to confirm our experimental conditions. We have made considerable progress and will be performing initial ChIP-sequencing experiments. Jyoti Narayana, PhD. Subscribe to: Posts (Atom). Horton Lab - Home. Jyoti Narayana, PhD. View my complete profile.
