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OpenOptogenetics | The blog

March 17, 2014. Two animal models were employed: a transgenic model that expresses ChR2 under control of the Thy1.2 promoter in auditory neurons and an adeno-associated virus-mediated model that expressed the ChR2 variant CatCh (AAV2/6-hSyn-CatCh-YFP). Optogenetic stimulation of spiral ganglion neurons (SGN) evoked auditory brainstem responses both in normal hearing mice and in mouse models of deafness, suggesting the general feasability of cochlear optogenetics for restoring auditory activity. The autho...

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March 17, 2014. Two animal models were employed: a transgenic model that expresses ChR2 under control of the Thy1.2 promoter in auditory neurons and an adeno-associated virus-mediated model that expressed the ChR2 variant CatCh (AAV2/6-hSyn-CatCh-YFP). Optogenetic stimulation of spiral ganglion neurons (SGN) evoked auditory brainstem responses both in normal hearing mice and in mouse models of deafness, suggesting the general feasability of cochlear optogenetics for restoring auditory activity. The autho...

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OpenOptogenetics | The blog | openoptogeneticsblog.org Reviews

https://openoptogeneticsblog.org

March 17, 2014. Two animal models were employed: a transgenic model that expresses ChR2 under control of the Thy1.2 promoter in auditory neurons and an adeno-associated virus-mediated model that expressed the ChR2 variant CatCh (AAV2/6-hSyn-CatCh-YFP). Optogenetic stimulation of spiral ganglion neurons (SGN) evoked auditory brainstem responses both in normal hearing mice and in mouse models of deafness, suggesting the general feasability of cochlear optogenetics for restoring auditory activity. The autho...

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greenfluorescentblog | A blog on fluorescent microscopy in biological research | Page 2

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A blog on fluorescent microscopy in biological research. Newer posts →. ASCB15 – part 3. January 4, 2016. I ended part 2 Monday night. It was an exciting day with many excellent talks, but the best talk (mine, of course! Was due the next day. Continue reading →. ASCB15 – part 2. December 31, 2015. I ended Part 1. After the morning session on pushing the boundaries of imaging. After this session, I attended my first discussion table . Continue reading →. December 17, 2015. Continue reading →. I have sever...

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Tracking membranes by imaging – mCLING and surface glycans | greenfluorescentblog

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A blog on fluorescent microscopy in biological research. Visualizing translation: insert TRICK pun here. Tracking membranes by imaging – mCLING and surface glycans. April 14, 2015. Living cells exhibit many types of membranes which participate in most biological precesses, one way or another. Imaging membranes is usually acheived by two types of reagents: chemical dyes or fluorescent proteins that are targeted to the membrane itself or inside an organelle. Tetramethylrosamine, and Mitotracker. Bind the p...

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A blog on fluorescent microscopy in biological research. Calculate pixel size on images. Choose the right fluorescent protein ( part 1. Calculate numerical aperture and resolution. FISH for RNA ( basics. Basics of spot analysis. Click to email (Opens in new window). Click to print (Opens in new window). Click to share on Google (Opens in new window). Share on Facebook (Opens in new window). Click to share on Twitter (Opens in new window). Click to share on LinkedIn (Opens in new window). Fill in your det...

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Corrigendum | greenfluorescentblog

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A blog on fluorescent microscopy in biological research. Tracking membranes by imaging – mCLING and surface glycans. Using Thresholds to Measure and Quantify Cells in Image J →. August 4, 2015. The paper then goes through several rounds of changes, following reviewers’ comments, editor’s comments, final proof-reading etc…. The accepted manuscript (MS) should be “perfect”. But mistakes can happen. Mistakes did. Happen for three of my recent papers. For my Cell paper. Then, we published a paper in PLOS One.

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March 17, 2014. Two animal models were employed: a transgenic model that expresses ChR2 under control of the Thy1.2 promoter in auditory neurons and an adeno-associated virus-mediated model that expressed the ChR2 variant CatCh (AAV2/6-hSyn-CatCh-YFP). Optogenetic stimulation of spiral ganglion neurons (SGN) evoked auditory brainstem responses both in normal hearing mice and in mouse models of deafness, suggesting the general feasability of cochlear optogenetics for restoring auditory activity. The autho...

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